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Type HazMat fee for 500 gram (Estimated)
Excepted Quantity USD 0.00
Limited Quantity USD 15-60
Inaccessible (Haz class 6.1), Domestic USD 80+
Inaccessible (Haz class 6.1), International USD 150+
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Accessible (Haz class 3, 4, 5 or 8), International USD 200+
Chemical Structure| 64091-91-4 Chemical Structure| 64091-91-4

Structure of NNK
CAS No.: 64091-91-4

Chemical Structure| 64091-91-4

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NNK is a nitrosation derivative of nicotine that activates ERK1/2 and PKCα, and activates Bcl2 phosphorylation at Ser70, and c-Myc at Thr58 and Ser62. NNK can induce the proliferation and survival of human lung cancer cells and is used to construct a mouse model of lung cancer.

4.5 *For Research Use Only !

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Product Citations

Product Citations

Boyao Zhang ; George-Eugen Maftei ; Bartosz Bartmanski ; Michael Zimmermann ;

Abstract: Organic carcinogens, in particular DNA-reactive compounds, contribute to the irreversible initiation step of tumorigenesis through introduction of genomic instability. Although carcinogen bioactivation and detoxification by human enzymes has been extensively studied, carcinogen biotransformation by human-associated bacteria, the microbiota, has not yet been systematically investigated. We tested the biotransformation of 68 mutagenic carcinogens by 34 bacterial species representative for the upper and lower human gastrointestinal tract and found that the majority (41) of the tested carcinogens undergo bacterial biotransformation. To assess the functional consequences of microbial carcinogen metabolism, we developed a pipeline to couple gut bacterial carcinogen biotransformation assays with Ames mutagenicity testing and liver biotransformation experiments. This revealed a bidirectional crosstalk between gut microbiota and host carcinogen metabolism, which we validated in gnotobiotic mouse models. Overall, the systematic assessment of gut microbiota carcinogen biotransformation and its interplay with host metabolism highlights the gut microbiome as an important modulator of exposome-induced tumorigenesis.

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Product Details of NNK

CAS No. :64091-91-4
Formula : C10H13N3O2
M.W : 207.23
SMILES Code : O=C(C1=CC=CN=C1)CCCN(N=O)C
MDL No. :MFCD00274580
InChI Key :FLAQQSHRLBFIEZ-UHFFFAOYSA-N
Pubchem ID :47289

Safety of NNK

GHS Pictogram:
Signal Word:Danger
Hazard Statements:H301-H317-H351
Precautionary Statements:P280-P301+P310
Class:6.1
UN#:2811
Packing Group:

Isoform Comparison

Biological Activity

Description
NNK, a nitrosated derivative of nicotine, concurrently induces phosphorylation of Bcl2 uniquely at Ser70 and c-Myc at Thr58 and Ser62 via activation of both ERK1/2 and PKCα[1].

Protocol

Bio Calculators
Preparing Stock Solutions 1mg 5mg 10mg

1 mM

5 mM

10 mM

4.83mL

0.97mL

0.48mL

24.13mL

4.83mL

2.41mL

48.26mL

9.65mL

4.83mL

Dissolving Methods
The prepared working fluid is recommended to be prepared now and used up as soon as possible in a short period of time. The percentage shown in front of the following solvent refers to the volume ratio of the solvent in the final solution; If precipitation or precipitation occurs in the preparation process, it can be assisted by heating and/or ultrasound.
Protocol 1

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