GPR35 is a member of the G protein-coupled receptor family, characterized by the typical seven transmembrane domains. It is expressed in various cells and tissues, including the intestines, immune cells, heart, and central nervous system. GPR35 is involved in regulating inflammatory responses, pain perception, cardiovascular functions, and energy metabolism. Through the activation of Gαi/o proteins and the β-arrestin pathway, GPR35 participates in multiple signaling pathways. The receptor is sensitive to various endogenous and exogenous ligands, such as kynurenic acid. Its gene expression exhibits tissue specificity and is associated with the risk and progression of cardiovascular diseases, inflammation, and neurodegenerative disorders. Mutations or changes in expression may impact the pathogenesis of related diseases.