PFKFB3 is a member of the phosphofructokinase-2/fructose-2,6-bisphosphatase family, responsible for the synthesis of fructose-2,6-bisphosphate, a key regulator of glycolysis. It is primarily expressed in cancer cells, myocardial, and brain tissues, regulating glucose metabolism to support the demands of rapidly growing cells. PFKFB3 activity is regulated by various signaling pathways, such as the insulin signaling pathway. Specific changes in expression or gene mutations can lead to metabolic disorders, associated with cancer progression, cardiovascular diseases, and other health issues.