The choline transporter (CHT) is a critical neurotransmitter transporter responsible for transporting choline from the extracellular space back into the presynaptic cell, to be reused in the acetylation reaction for synthesizing acetylcholine anew. CHT belongs to a single-member family, composed of a single polypeptide chain, and is primarily located on the presynaptic membrane of cholinergic neurons. This process is essential for maintaining the function of the cholinergic nervous system and is involved in a variety of physiological responses, including muscle control, memory formation, and attention regulation. The expression of the CHT gene is tissue-specific, and its activity is regulated by neural activity and signaling pathways. Gene mutations or dysregulation of expression are associated with a range of neurological disorders, such as neurodegenerative diseases and Attention Deficit Hyperactivity Disorder (ADHD).