The Src family of kinases includes Src, Fyn, Yes, and others, which possess SH3, SH2 domains, and tyrosine kinase catalytic domains. They are widely expressed in various cells and tissues, particularly regulating cell adhesion, proliferation, differentiation, and survival near the cell membrane. The Src family regulates cellular behavior by participating in multiple signaling pathways such as Ras/MAPK, PI3K/Akt, and cytoskeletal reorganization. The specific expression patterns and activity regulation of related genes are associated with the development of cancer, immune responses, osteoporosis, and other diseases. Mutations or overactivation of Src family members have been observed in various tumors, making them potential therapeutic targets.