LpxC (Lipid A Synthase C) is a crucial enzyme within the metal-dependent fatty acid synthase family, primarily involved in the bacterial lipopolysaccharide biosynthesis pathway. It is a zinc-containing metalloenzyme with multiple structural domains to ensure catalytic efficiency. LpxC is predominantly localized in the bacterial cytoplasm and promotes the initial synthesis of lipopolysaccharides by catalyzing the deacetylation of UDP-3-O-((R)-3-hydroxymyristoyl) glucosamine. The enzyme’s activity and expression are tightly regulated to adapt to environmental changes. In some bacteria, genetic mutations may affect LpxC functionality, leading to increased antibiotic resistance.