Ceramidases are important enzymes widely distributed in cellular membranes and endomembrane systems. Their primary function is to hydrolyze the amide bond of ceramides, generating fatty acids and sphingosine. These products play critical roles in cellular signal transduction, influencing activities like apoptosis, cell proliferation, and the cell cycle. Ceramidases regulate cellular sphingolipid levels and participate in multiple signaling pathways including inflammation, intracellular transport, and apoptosis regulation.