AIMP2-DX2 is a splice variant of Aminoacyl-tRNA Synthetase Interacting Multifunctional Protein 2 (AIMP2) that lacks a portion of its functional domain, thereby affecting its normal role in protein synthesis. This protein has specific expression and localization in the cytoplasm, playing a key role in biological processes such as cell proliferation and apoptosis, especially by modulating these processes through the impact on signaling pathways like p53 and NF-κB. In certain types of cancer, the expression of AIMP2-DX2 is abnormal, suggesting it may play a promotive role in tumor initiation and progression. Understanding the expression patterns and any potential genetic variations of AIMP2-DX2 is crucial for elucidating its role in diseases and may become a target for future therapeutic strategies.