The ADP-ribosyltransferase (ADPRT) family is a group of enzymes responsible for catalyzing the transfer of ADP-ribose from NAD+ to substrate proteins. Family members include ARTD (or PARP) and ARTC, which possess different domains such as catalytic and ribose-binding domains, and are primarily located in the nucleus and cytoplasm. These enzymes are involved in biological processes such as DNA repair, cell signaling, and gene expression regulation, affecting protein function and cellular responses through ADP-ribosylation. The expression of ADP-ribosyltransferase family members is tissue-specific, and mutations or dysregulation in their genes are associated with various diseases, including cancer, neurodegenerative diseases, and inflammation.