The Terminal Nucleotidyltransferase (TENT) family, also known as TENT1 to TENT5 or TUT1 to TUT5, is a group of enzymes involved in RNA metabolism. They possess various structural domains, including nucleotidyltransferase domains and RNA-binding domains, primarily located in the cell nucleus. The TENT family participates in the 3' end processing of RNA, including the maturation of mRNA, miRNA, and piRNA, regulating gene expression by influencing RNA stability and function. The expression of the TENT family is tissue-specific, and mutations or dysregulation are associated with various diseases, including cancer, neurodegenerative diseases, and reproductive developmental abnormalities.