c-Myc is a member of the Myc family, which includes c-Myc, N-Myc, and L-Myc. It is an important transcription factor that regulates the expression of multiple genes by binding to E-box DNA sequences. It contains a transcription activation domain, a DNA-binding domain, and a leucine zipper domain. c-Myc is primarily located in the cell nucleus, where it regulates cell proliferation, growth, differentiation, and apoptosis. It is expressed in various cells and tissues, and its overexpression or gene mutations are closely associated with the occurrence of various cancers. The activity of c-Myc is regulated by a wide range of upstream signaling pathways such as Ras/MAPK and PI3K/Akt. The specific expression and functional abnormalities of related genes are key factors in malignant cell transformation, making c-Myc a potential target for cancer therapy.