The Cdc25 (Cell Division Cycle 25) family consists of key cell cycle-regulating phosphatases, including Cdc25A, Cdc25B, and Cdc25C. These proteins are primarily located in the nucleus and function to dephosphorylate and activate cyclin-dependent kinases (CDKs), thereby driving cell cycle progression. Cdc25 operates by regulating transitions from G1 to S phase and G2 to M phase, serving as critical nodes in cell cycle control. Their gene expression is precisely regulated throughout the cell cycle, with mutations or abnormal expressions linked to the development of various cancers, particularly in processes controlling cell division and proliferation.