Secretases are a group of proteolytic enzymes responsible for cleaving transmembrane proteins. They mainly include β-secretase (BACE1) and the γ-secretase complex. β-secretase is primarily expressed in the Golgi apparatus and endoplasmic reticulum, while the γ-secretase complex is expressed on the cell membrane. Secretases are involved in the cleavage of Amyloid Precursor Protein (APP), generating β-amyloid, which is closely related to the pathogenesis of Alzheimer's disease. Additionally, they participate in the Notch signaling pathway and cell fate determination. The expression of secretase genes is tissue-specific, and mutations or abnormal activity are associated with various neurodegenerative diseases and cancers.