Asparagine synthetase (ASNS) is an enzyme responsible for catalyzing the formation of asparagine from aspartate and glutamine, utilizing ammonia, to synthesize asparagine. It consists of multiple functional domains and is primarily located in the cytoplasm. ASNS plays a crucial role in regulating amino acid metabolism and protein synthesis, especially under conditions of limited amino acid availability. In different tissues and cells, the expression of ASNS is specific, and mutations or dysregulation in its expression are associated with various diseases, including cancer, immune deficiencies, and metabolic disorders. The activity of ASNS is regulated by cellular nutrient status and amino acid availability, affecting the cell's adaptability and survival.