The KLF (Krüppel-like Factor) family comprises at least 17 members, which regulate gene expression by binding to DNA through three C2H2-type zinc finger domains. KLF proteins localize in the cell nucleus and are involved in regulating various physiological and pathological processes such as cell proliferation, differentiation, growth, apoptosis, and response. Family members like KLF4 and KLF2 play significant roles in vascular biology, epidermal development, and stem cell maintenance. The diverse functionality of KLF genes corresponds to their specific expression patterns, with different members exhibiting varying expression levels in different cell types and tissues. Dysregulation of KLF family member expression or gene mutations is associated with various diseases including cancer, cardiovascular diseases, and metabolic disorders.