Choline kinase is a key enzyme involved in the synthesis of phosphatidylcholine, playing a crucial role in cell membrane formation and cell signaling. It catalyzes the reaction between choline and ATP to produce phosphocholine, which then participates in the production of phosphatidylcholine. The choline kinase family includes several isoforms, primarily expressed in the liver, neural cells, and muscle cells. The localization of these enzymes in different cells and tissues is closely related to their functions, regulating cell proliferation, differentiation, and signal transduction. The specificity of gene expression and mutations can affect the activity of choline kinase, potentially linking it to the development and progression of various diseases, such as cancer and neurodegenerative disorders.