MLKL (Mixed Lineage Kinase Domain-Like Protein) is a key executor protein in the necroptotic cell death pathway, inducing cell death by forming pores in the cell membrane. Comprising an N-terminal four-helical bundle domain and a C-terminal kinase-like domain, MLKL is primarily located in the cytoplasm but can translocate to the cell membrane upon activation. It plays a significant role in regulating inflammatory responses and the immune system. The specific expression and mutations of the MLKL gene are associated with the development of various diseases, including autoimmune diseases and certain cancers.