The PAM complex is crucial for protein translocation across membranes and insertion into the endoplasmic reticulum membrane. Comprising multiple subunits, including the Sec61 channel, TRAP complex, and Ost complex, it resides at the ER membrane. The PAM complex assists in the proper folding and insertion of newly synthesized proteins into the ER membrane, playing a role in protein processing and quality control mechanisms. Specific expression and mutations of certain genes can affect the functionality of the PAM complex, leading to aberrations in protein processing and signaling pathways, and are associated with various diseases.