LC3 (Microtubule-associated proteins 1A/1B light chain 3) is an autophagy-related protein and part of the ATG8 family, consisting of three forms: LC3A, LC3B, and LC3C. LC3 conjugates covalently with phosphatidylethanolamine (PE) to form LC3-II, localizing to autophagosome membranes. This conversion is a critical step in the autophagy process, involving intracellular degradation and recycling. LC3 expression is regulated by cellular nutritional status and stress levels, and specific mutations can affect the autophagy process, associated with various diseases including neurodegenerative disorders.