Home Products Cited in Publications Worldwide Direct Modulators of K-Ras-Membrane Interactions
ACS Chem. Biol.,2023,18(9):2082-2093.
Morstein, Johannes; Shrestha, Rebika; Van, Que N.; Lopez, Cesar A.; Arora, Neha; Tonelli, Marco; Liang, Hong; Chen, De; Zhou, Yong; Hancock, John F.; Stephen, Andrew G.; Turbyville, Thomas J.; Shokat, Kevan M.
DOI:10.1021/acschembio.3c00413 PMID:37579045
Protein-membrane interactions (PMIs) are ubiquitous in cellular signaling. Initial steps of signal transduction cascades often rely on transient and dynamic interactions with the inner plasma membrane leaflet to populate and regulate signaling hotspots. Methods to target and modulate these interactions could yield attractive tool compounds and drug candidates. Here, the conjugation of a medium-chain lipid tail to the covalent K-Ras(G12C) binder MRTX849 at a solvent-exposed site enables such direct modulation of PMIs. The conjugated lipid tail interacts with the tethered membrane and changes the relative membrane orientation and conformation of K-Ras(G12C), as shown by mol. dynamics (MD) simulation-supported NMR studies. In cells, this PMI modulation restricts the lateral mobility of K-Ras(G12C) and disrupts nanoclusters. The described strategy could be broadly applicable to selectively modulate transient PMIs.