Home Products Cited in Publications Worldwide A Method for Converting HPLC Peak Area from Online Reaction Monitoring to Concentration Using Nonlinear Regression
Deem, Madeleine C.; Hein, Jason E.
DOI:10.1021/acs.joc.2c02737 PMID:36625157
Online HPLC reaction progress monitoring provides detailed data-rich profiles; however, extracting kinetic information requires UV-visible response factors to determine concentrations from peak areas. If the reaction's overall mass balance is known and some anal. trend for all relevant species can be recorded, it is possible to estimate the absolute response factors of all species using a system of linear equations. We delineate a method using the Microsoft Solver plug-in to convert time course profiles to reagent concentrations without anal. standards