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Type HazMat fee for 500 gram (Estimated)
Excepted Quantity USD 0.00
Limited Quantity USD 15-60
Inaccessible (Haz class 6.1), Domestic USD 80+
Inaccessible (Haz class 6.1), International USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic USD 100+
Accessible (Haz class 3, 4, 5 or 8), International USD 200+
Chemical Structure| 1895922-69-6 Chemical Structure| 1895922-69-6
Chemical Structure| 1895922-69-6

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Aminooxy-PEG1-propargyl is a 1-unit PEG derivative with an aminooxy group and a propargyl group. It is widely used in bioconjugation, drug delivery, and peptide synthesis.

Synonyms: Aminooxy-PEG1-propargyl

4.5 *For Research Use Only !

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Product Citations

Product Citations

Wolfer, Jamison D ; Minkoff, Benjamin B ; Burch, Heather L ; Sussman, Michael R ;

Abstract: Protein footprinting is a useful method for studying protein higher order structure and conformational changes induced by interactions with various ligands via addition of covalent modifications onto the protein. Compared to other methods that provide single amino acid-level structural resolution, such as cryo-EM, X-ray diffraction, and NMR, mass spectrometry (MS)-based methods can be advantageous as they require lower protein amounts and purity. As with other MS-based proteomic methods, such as post-translational modification analysis, enrichment techniques have proven necessary for both optimal sensitivity and sequence coverage when analyzing highly complex proteomes. Currently used reagents for footprinting via covalent labeling, such as hydroxyl radicals and carbodiimide-based methods, do not yet have a suitable enrichment method, limiting their applicability to whole proteome analysis. Here, we report a method for enrichable covalent labeling built upon the GEE/EDC system commonly used to covalently label aspartic acid and glutamic acid residues. Novel labeling reagents containing alkynyl functionality can be "clicked" to any azido-containing molecule with copper-catalyzed azide−alkyne cycloaddition (CuAAC), allowing for enrichment or further labeling. Multiple azide- and alkyne-containing GEE-like molecules were tested, and the most efficient method was determined to be the EDC-facilitated coupling of glycine propargyl amide (GPA) to proteins. The pipeline we report includes clicking via CuAAC to a commercially available biotin-azide containing a photocleavable linker, followed by enrichment using a streptavidin resin and subsequent cleavage under ultraviolet light. The enrichment process was optimized through the screening of clickable amines, coupling reagents, and enrichment scaffolds and methods with pure model proteins and has also been applied to complex mixtures of proteins isolated from the model plant, Arabidopsis thaliana, suggesting that our method may ultimately be used to measure protein conformation on a proteomic scale.

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Product Details of Aminooxy-PEG1-propargyl

CAS No. :1895922-69-6
Formula : C5H9NO2
M.W : 115.13
SMILES Code : C(CON)OCC#C
Synonyms :
Aminooxy-PEG1-propargyl
MDL No. :MFCD29764324
InChI Key :HIPBEDYVWCVJGB-UHFFFAOYSA-N
Pubchem ID :119058269

Safety of Aminooxy-PEG1-propargyl

GHS Pictogram:
Signal Word:Danger
Hazard Statements:H302-H312-H332-H335-H314
Precautionary Statements:P260-P264-P270-P271-P280-P301+P330+P331-P302+P352-P303+P361+P353-P304+P340-P305+P351+P338-P310-P363-P403+P233-P405-P501
Class:8
UN#:2735
Packing Group:
 

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