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MTT (thiazolyl blue tetrazolium bromide), a yellow dye, is used in measurement of cell proliferation. Succinate dehydrogenase in living cell mitochondria can reduce exogenous MTT to water-insoluble blue-purple crystalline formamidine.
Synonyms: MTT;Thiazolyl Blue Tetrazolium bromide;NSC 60102
4.5
*For Research Use Only !
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Batch number can be found on the product's label following the word 'Batch'.
Search for reports by entering the product batch number.
Batch number can be found on the product's label following the word 'Batch'.
Search for reports by entering the product batch number.
Batch number can be found on the product's label following the word 'Batch'.
MTT (thiazolyl blue tetrazolium bromide), a yellow dye, is used in measurement of cell proliferation. Succinate dehydrogenase in living cell mitochondria can reduce exogenous MTT to water-insoluble blue-purple crystalline formamidine.
Synonyms: MTT;Thiazolyl Blue Tetrazolium bromide;NSC 60102
4.5
*For Research Use Only !
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Search for reports by entering the product batch number.
Batch number can be found on the product's label following the word 'Batch'.
Search for reports by entering the product batch number.
Batch number can be found on the product's label following the word 'Batch'.
Search for reports by entering the product batch number.
Batch number can be found on the product's label following the word 'Batch'.
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Light-Controlled Anticancer Activity and Cellular Uptake of a Photoswitchable Cisplatin Analogue
Marta Stolarek ; Kamil Kaminski ; Marta Kaczor-Kamińska , et al. JMC,2024,67(21):19103-19120.
Abstract: A photoactive analogue of cisplatin was synthesized with two arylazopyrazole ligands, able to undergo trans−cis/cis−trans photoisomerizations. The cis photoisomer showed a dark half-life of 9 days. The cytotoxicities of both photoisomers of the complex were determined in several cancer and normal cell lines and compared to that of cisplatin. The trans photoisomer of the complex was much more cytotoxic than both the cis photoisomer and cisplatin, and was more toxic for cancer (4T1) than for normal (NMuMG) murine breast cells. 4T1 cell death occurred through necrosis. Photoisomerization of the trans and cis photoisomers internalized by the 4T1 cells increased and decreased their viability, respectively. The cellular uptake of the trans photoisomer was stronger than that of both the cis photoisomer and cisplatin. Both photoisomers interacted with DNA faster than cisplatin. The trans photoisomer was bound stronger by bovine serum albumin and induced a greater decrease in cellular glutathione levels than the cis photoisomer.
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Purchased from AmBeed: 298-93-1 ; 15663-27-1
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Ameerah A. Radhi ; Wedad K. Ali ; Fitua Al-Saedi Al Mustansiriyah Journal of Pharmaceutical Sciences,2023,23(1):58-67.
Abstract: Tamoxifen Citrate (TC) is the standard endocrine therapy for estrogen receptor (ER) positive breast cancer. TC is a selective estrogen receptor modulator (SERM) whose estrogenic properties in uterus have been linked to increased side effects like blood clots, endometrial polyps and cancer. Therefore, significant amount of research has been carried out to develop tamoxifen loaded nano-formulations with a preferential accumulation in tumor tissue rather than healthy tissues. Synthetic high-density lipoproteins (sHDL) are novel nanocarriers with inherent active-targeting ability towards tumor cells through the ligand–receptor interaction between apolipoprotein A-I (Apo A-I) and scavenger receptor class B type I (SR-BI) overexpressed in various malignant cells. The current study was carried out to investigate whether encapsulation of TC in sHDL could improve the cytotoxic effect of TC against malignant cells. For this purpose, the cytotoxicity of TC-sHDL was evaluated in MCF-7 cell line in vitro. MTT assay demonstrated the increased cytotoxicity of TC-sHDL against cancer cells as compared with the cytotoxic effect of the free drug.
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Keywords: Tamoxifen Citrate ; breast cancer ; in vitro cytotoxicity
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Purchased from AmBeed: 298-93-1
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CAS No. : | 298-93-1 |
Formula : | C18H16BrN5S |
M.W : | 414.32 |
SMILES Code : | CC1=C(C)N=C(N2[N+](C3=CC=CC=C3)=NC(C4=CC=CC=C4)=N2)S1.[Br-] |
Synonyms : |
MTT;Thiazolyl Blue Tetrazolium bromide;NSC 60102
|
MDL No. : | MFCD00011964 |
InChI Key : | AZKSAVLVSZKNRD-UHFFFAOYSA-M |
Pubchem ID : | 64965 |
GHS Pictogram: | ![]() ![]() |
Signal Word: | Warning |
Hazard Statements: | H315-H319-H335-H341 |
Precautionary Statements: | P201-P202-P261-P302+P352-P305+P351+P338-P308+P313 |
Num. heavy atoms | 25 |
Num. arom. heavy atoms | 22 |
Fraction Csp3 | 0.11 |
Num. rotatable bonds | 3 |
Num. H-bond acceptors | 3.0 |
Num. H-bond donors | 0.0 |
Molar Refractivity | 104.99 |
TPSA ? Topological Polar Surface Area: Calculated from | 75.72 Ų |
Log Po/w (iLOGP)? iLOGP: in-house physics-based method implemented from | -2.39 |
Log Po/w (XLOGP3)? XLOGP3: Atomistic and knowledge-based method calculated by | 7.5 |
Log Po/w (WLOGP)? WLOGP: Atomistic method implemented from | 0.29 |
Log Po/w (MLOGP)? MLOGP: Topological method implemented from | 3.92 |
Log Po/w (SILICOS-IT)? SILICOS-IT: Hybrid fragmental/topological method calculated by | 3.7 |
Consensus Log Po/w? Consensus Log Po/w: Average of all five predictions | 2.6 |
Log S (ESOL):? ESOL: Topological method implemented from | -7.59 |
Solubility | 0.0000107 mg/ml ; 0.0000000259 mol/l |
Class? Solubility class: Log S scale | Poorly soluble |
Log S (Ali)? Ali: Topological method implemented from | -8.92 |
Solubility | 0.000000493 mg/ml ; 0.0000000012 mol/l |
Class? Solubility class: Log S scale | Poorly soluble |
Log S (SILICOS-IT)? SILICOS-IT: Fragmental method calculated by | -6.04 |
Solubility | 0.000381 mg/ml ; 0.00000092 mol/l |
Class? Solubility class: Log S scale | Poorly soluble |
GI absorption? Gatrointestinal absorption: according to the white of the BOILED-Egg | High |
BBB permeant? BBB permeation: according to the yolk of the BOILED-Egg | No |
P-gp substrate? P-glycoprotein substrate: SVM model built on 1033 molecules (training set) | Yes |
CYP1A2 inhibitor? Cytochrome P450 1A2 inhibitor: SVM model built on 9145 molecules (training set) | Yes |
CYP2C19 inhibitor? Cytochrome P450 2C19 inhibitor: SVM model built on 9272 molecules (training set) | Yes |
CYP2C9 inhibitor? Cytochrome P450 2C9 inhibitor: SVM model built on 5940 molecules (training set) | No |
CYP2D6 inhibitor? Cytochrome P450 2D6 inhibitor: SVM model built on 3664 molecules (training set) | No |
CYP3A4 inhibitor? Cytochrome P450 3A4 inhibitor: SVM model built on 7518 molecules (training set) | No |
Log Kp (skin permeation)? Skin permeation: QSPR model implemented from | -3.5 cm/s |
Lipinski? Lipinski (Pfizer) filter: implemented from | 0.0 |
Ghose? Ghose filter: implemented from | None |
Veber? Veber (GSK) filter: implemented from | 0.0 |
Egan? Egan (Pharmacia) filter: implemented from | 0.0 |
Muegge? Muegge (Bayer) filter: implemented from | 1.0 |
Bioavailability Score? Abbott Bioavailability Score: Probability of F > 10% in rat | 0.55 |
PAINS? Pan Assay Interference Structures: implemented from | 0.0 alert |
Brenk? Structural Alert: implemented from | 1.0 alert: heavy_metal |
Leadlikeness? Leadlikeness: implemented from | No; 1 violation:MW<2.0 |
Synthetic accessibility? Synthetic accessibility score: from 1 (very easy) to 10 (very difficult) | 3.54 |
Tags: 298-93-1 synthesis path| 298-93-1 SDS| 298-93-1 COA| 298-93-1 purity| 298-93-1 application| 298-93-1 NMR| 298-93-1 COA| 298-93-1 structure
A149017 [34176-31-3]
5-Methyl-4-phenylthiazol-2-amine hydrobromide
Similarity: 0.55
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P222 | Do not allow contact with air. |
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P232 | Protect from moisture. |
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P270 | Do not eat, drink or smoke when using this product. |
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P272 | Contaminated work clothing should not be allowed out of the workplace. |
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P283 | Wear fire/flame resistant/retardant clothing. |
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P391 | Collect spillage. Hazardous to the aquatic environment |
P301 + P310 | IF SWALLOWED: Immediately call a POISON CENTER or doctor/physician. |
P301 + P312 | IF SWALLOWED: call a POISON CENTER or doctor/physician IF you feel unwell. |
P301 + P330 + P331 | IF SWALLOWED: Rinse mouth. Do NOT induce vomiting. |
P302 + P334 | IF ON SKIN: Immerse in cool water/wrap in wet bandages. |
P302 + P350 | IF ON SKIN: Gently wash with plenty of soap and water. |
P303 + P361 + P353 | IF ON SKIN (or hair): Remove/Take off Immediately all contaminated clothing. Rinse SKIN with water/shower. |
P304 + P312 | IF INHALED: Call a POISON CENTER or doctor/physician if you feel unwell. |
P304 + P340 | IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing. |
P304 + P341 | IF INHALED: If breathing is difficult, remove victim to fresh air and keep at rest in a position comfortable for breathing. |
P305 + P351 + P338 | IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. |
P306 + P360 | IF ON CLOTHING: Rinse Immediately contaminated CLOTHING and SKIN with plenty of water before removing clothes. |
P307 + P311 | IF exposed: call a POISON CENTER or doctor/physician. |
P308 + P313 | IF exposed or concerned: Get medical advice/attention. |
P309 + P311 | IF exposed or if you feel unwell: call a POISON CENTER or doctor/physician. |
P332 + P313 | IF SKIN irritation occurs: Get medical advice/attention. |
P333 + P313 | IF SKIN irritation or rash occurs: Get medical advice/attention. |
P335 + P334 | Brush off loose particles from skin. Immerse in cool water/wrap in wet bandages. |
P337 + P313 | IF eye irritation persists: Get medical advice/attention. |
P342 + P311 | IF experiencing respiratory symptoms: call a POISON CENTER or doctor/physician. |
P370 + P376 | In case of fire: Stop leak if safe to Do so. |
P370 + P378 | In case of fire: |
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P370 + P380 + P375 | In case of fire: Evacuate area. Fight fire remotely due to the risk of explosion. |
P371 + P380 + P375 | In case of major fire and large quantities: Evacuate area. Fight fire remotely due to the risk of explosion. |
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P413 | |
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H231 | May react explosively even in the absence of air at elevated pressure and/or temperature |
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H242 | Heating may cause a fire |
H250 | Catches fire spontaneously if exposed to air |
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H252 | Self-heating in large quantities; may catch fire |
H260 | In contact with water releases flammable gases which may ignite spontaneously |
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H311 | Toxic in contact with skin |
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H314 | Causes severe skin burns and eye damage |
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H412 | Harmful to aquatic life with long-lasting effects |
H413 | May cause long-lasting harmful effects to aquatic life |
H420 | Harms public health and the environment by destroying ozone in the upper atmosphere |
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