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Structure of 24423-98-1
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The BI-3802 was designed by Boehringer Ingelheim and could be obtained free of charge through the Boehringer Ingelheim open innovation portal opnMe.com, associated with its negative control.
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CAS No. : | 24423-98-1 |
Formula : | C3H5ClO2 |
M.W : | 108.52 |
SMILES Code : | O=C(CO)CCl |
MDL No. : | MFCD19232085 |
GHS Pictogram: |
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Signal Word: | Danger |
Hazard Statements: | H302-H314 |
Precautionary Statements: | P264-P270-P271-P280-P303+P361+P353-P304+P340-P305+P351+P338-P310-P330-P331-P363-P403+P233-P501 |
Class: | 8 |
UN#: | 3261 |
Packing Group: | Ⅱ |
* All experimental methods are cited from the reference, please refer to the original source for details. We do not guarantee the accuracy of the content in the reference.
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
With Serratia-derived glycerol dehydrogenase; NADH; at 30℃; for 2h;pH 6.5;Aqueous phophate buffer; Enzymatic reaction; | (Example 9) Method for synthesizing (S)-3-chloro-1,2-propanediol A Serratia-derived glycerol dehydrogenase solution was obtained from a culture broth of Serratia marcescens IFO12648 strains by a purification operation comprising ammonium sulfate fractionation and ion-exchange chromatography. 1 ml of a 0.1M phosphate buffer (PH 6.5) containing 10 mul of the thus-obtained glycerol dehydrogenase solution, 1.5 mg of 1-chloro-3-hydroxyacetone, and 10 mg of NADH was stirred at 30C for 2 hours.. After the reaction was completed, the reaction solution was saturated with ammonium sulfate, and extracted with an equivalent volume of ethyl acetate.. The amounts of the substrate and product in the extract were measured by gas chromatography (GC) analysis to determine the conversion rate (%).. Also, the optical purity (%ee) of the product was measured by high-performance liquid chromatography (HPLC) analysis after the primary hydroxyl group of the product was tosylated with tosyl chloride.. As a result, (S)-3-chloro-1,2-propanediol was produced with a conversion rate of 100% and an optical purity of 100%ee.. | |
With potassium dihydrogenphosphate; D-glucose; In water; at 30℃; for 24h;pH 5.5;Aqueous phophate buffer; Microbiological reaction; | (Example 1) A liquid medium (pH 7.0) containing 4% of glucose, 0.3% of yeast extract, 1.3% of KH2PO4, 0.7% of (NH4)2HPO4, 0.01% of NaCl, 0.08% of MgSO4·7H2O, 0.006% of ZnSO4·7H2O, 0.009% of FeSO4·7H2O, 0.0005% of CuSO4·5H2O, and 0.001% of MnSO4·4-5H2O was prepared, and 5 ml of the liquid medium was injected into each of large test tubes and then sterilized with steam at 120C for 20 minutes. Then, one platinum loop of the microorganisms of each of the genera shown in Table 1 and 2 was inoculated into the liquid medium, and cultured with shaking at 30C for 2 to 3 days. Cells were collected from each of the culture broths by centrifugation, washed with water and then suspended in 1 ml of a 0.1M phosphate buffer (pH 5.5). Then, 0.5 ml of the cell suspension was mixed with 0.5 ml of a 0.1M KH2PO4 aqueous solution containing 5 mg of 1-chloro-3-hydroxyacetone and 20 mg of glucose, and the resultant mixture was placed in a test tube with a stopper and shaken at 30C for 24 hours. After completion of reaction, a saturated amount of ammonium sulfate was added to the reaction solution, and extraction was performed with an equivalent volume of ethyl acetate. | |
With potassium dihydrogenphosphate; D-glucose; In water; at 30℃; for 24h;pH 5.5;Aqueous phophate buffer; Microbiological reaction; | (Example 1) A liquid medium (pH 7.0) containing 4% of glucose, 0.3% of yeast extract, 1.3% of KH2PO4, 0.7% of (NH4)2HPO4, 0.01% of NaCl, 0.08% of MgSO4·7H2O, 0.006% of ZnSO4·7H2O, 0.009% of FeSO4·7H2O, 0.0005% of CuSO4·5H2O, and 0.001% of MnSO4·4-5H2O was prepared, and 5 ml of the liquid medium was injected into each of large test tubes and then sterilized with steam at 120C for 20 minutes. Then, one platinum loop of the microorganisms of each of the genera shown in Table 1 and 2 was inoculated into the liquid medium, and cultured with shaking at 30C for 2 to 3 days. Cells were collected from each of the culture broths by centrifugation, washed with water and then suspended in 1 ml of a 0.1M phosphate buffer (pH 5.5). Then, 0.5 ml of the cell suspension was mixed with 0.5 ml of a 0.1M KH2PO4 aqueous solution containing 5 mg of 1-chloro-3-hydroxyacetone and 20 mg of glucose, and the resultant mixture was placed in a test tube with a stopper and shaken at 30C for 24 hours. After completion of reaction, a saturated amount of ammonium sulfate was added to the reaction solution, and extraction was performed with an equivalent volume of ethyl acetate. |
With potassium dihydrogenphosphate; D-glucose; In water; at 30℃; for 24h;pH 5.5;Aqueous phophate buffer; Microbiological reaction; | (Example 1) A liquid medium (pH 7.0) containing 4% of glucose, 0.3% of yeast extract, 1.3% of KH2PO4, 0.7% of (NH4)2HPO4, 0.01% of NaCl, 0.08% of MgSO4·7H2O, 0.006% of ZnSO4·7H2O, 0.009% of FeSO4·7H2O, 0.0005% of CuSO4·5H2O, and 0.001% of MnSO4·4-5H2O was prepared, and 5 ml of the liquid medium was injected into each of large test tubes and then sterilized with steam at 120C for 20 minutes. Then, one platinum loop of the microorganisms of each of the genera shown in Table 1 and 2 was inoculated into the liquid medium, and cultured with shaking at 30C for 2 to 3 days. Cells were collected from each of the culture broths by centrifugation, washed with water and then suspended in 1 ml of a 0.1M phosphate buffer (pH 5.5). Then, 0.5 ml of the cell suspension was mixed with 0.5 ml of a 0.1M KH2PO4 aqueous solution containing 5 mg of 1-chloro-3-hydroxyacetone and 20 mg of glucose, and the resultant mixture was placed in a test tube with a stopper and shaken at 30C for 24 hours. After completion of reaction, a saturated amount of ammonium sulfate was added to the reaction solution, and extraction was performed with an equivalent volume of ethyl acetate. | |
With D-glucose; NAD; In water;pH 7.0;Microbiological reaction; | (Example 12) Method for synthesizing (S)-3-chloro-1,2-propanediol Recombinant Escherichia coli HB101 (PNTSGG1) Accession No. FERM P-18449 were inoculated into 50 ml of sterilized 2*YT medium (16 g of tryptone, 10 g of yeast extract, 5 g of sodium chloride, and 1L of water, PH 7.0 before sterillization) in a 500 ml Sakaguchi flask, and cultured with shaking at 37C for 18 hours.. Then, 10 mg of 1-chloro-3-hydroxyacetone, 0.6 mg of NAD+, and 25 mg of glucose were added to 1 ml of the resultant culture broth, and the mixture was then stirred at 30C for 2 hours.. After the reaction was completed, the conversion rate and optical purity of the reaction product (S)-3-chloro-1,2-propanediol were analyzed by the same method as in Example 9.. As a result, the conversion rate was 97.1%, and the optical purity was 100%ee. | |
With Cellulomonas-derived glycerol dehydrogenase; NADH;Enzymatic reaction; | (Example 10) Method for synthesizing (S)-3-chloro-1,2-propanediol 1 ml of a 0.1M phosphate buffer (PH 6.5) containing 0.05 mg of Cellulomonas-derived glycerol dehydrogenase (produced by SIGMA), 1.5 mg of 1-chloro-3-hydroxyacetone, and 10 mg of NADH was stirred at 30C for 2 hours.. After the reaction was completed, the conversion rate and optical purity of the reaction product (S)-3-chloro-1,2-propanediol were analyzed by the same method as in Example 9.. As a result, the conversion rate was 100%, and the optical purity was 99.7%ee. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
With potassium dihydrogenphosphate; D-glucose; In water; at 30℃; for 24h;pH 5.5;Aqueous phophate buffer; Microbiological reaction; | (Example 1) A liquid medium (pH 7.0) containing 4% of glucose, 0.3% of yeast extract, 1.3% of KH2PO4, 0.7% of (NH4)2HPO4, 0.01% of NaCl, 0.08% of MgSO4·7H2O, 0.006% of ZnSO4·7H2O, 0.009% of FeSO4·7H2O, 0.0005% of CuSO4·5H2O, and 0.001% of MnSO4·4-5H2O was prepared, and 5 ml of the liquid medium was injected into each of large test tubes and then sterilized with steam at 120C for 20 minutes. Then, one platinum loop of the microorganisms of each of the genera shown in Table 1 and 2 was inoculated into the liquid medium, and cultured with shaking at 30C for 2 to 3 days. Cells were collected from each of the culture broths by centrifugation, washed with water and then suspended in 1 ml of a 0.1M phosphate buffer (pH 5.5). Then, 0.5 ml of the cell suspension was mixed with 0.5 ml of a 0.1M KH2PO4 aqueous solution containing 5 mg of 1-chloro-3-hydroxyacetone and 20 mg of glucose, and the resultant mixture was placed in a test tube with a stopper and shaken at 30C for 24 hours. After completion of reaction, a saturated amount of ammonium sulfate was added to the reaction solution, and extraction was performed with an equivalent volume of ethyl acetate. | |
With potassium dihydrogenphosphate; D-glucose; In water; at 30℃; for 24h;pH 5.5;Aqueous phophate buffer; Microbiological reaction; | (Example 1) A liquid medium (pH 7.0) containing 4% of glucose, 0.3% of yeast extract, 1.3% of KH2PO4, 0.7% of (NH4)2HPO4, 0.01% of NaCl, 0.08% of MgSO4·7H2O, 0.006% of ZnSO4·7H2O, 0.009% of FeSO4·7H2O, 0.0005% of CuSO4·5H2O, and 0.001% of MnSO4·4-5H2O was prepared, and 5 ml of the liquid medium was injected into each of large test tubes and then sterilized with steam at 120C for 20 minutes. Then, one platinum loop of the microorganisms of each of the genera shown in Table 1 and 2 was inoculated into the liquid medium, and cultured with shaking at 30C for 2 to 3 days. Cells were collected from each of the culture broths by centrifugation, washed with water and then suspended in 1 ml of a 0.1M phosphate buffer (pH 5.5). Then, 0.5 ml of the cell suspension was mixed with 0.5 ml of a 0.1M KH2PO4 aqueous solution containing 5 mg of 1-chloro-3-hydroxyacetone and 20 mg of glucose, and the resultant mixture was placed in a test tube with a stopper and shaken at 30C for 24 hours. After completion of reaction, a saturated amount of ammonium sulfate was added to the reaction solution, and extraction was performed with an equivalent volume of ethyl acetate. | |
With potassium dihydrogenphosphate; D-glucose; In water; at 30℃; for 24h;pH 5.5;Aqueous phophate buffer; Microbiological reaction; | (Example 1) A liquid medium (pH 7.0) containing 4% of glucose, 0.3% of yeast extract, 1.3% of KH2PO4, 0.7% of (NH4)2HPO4, 0.01% of NaCl, 0.08% of MgSO4·7H2O, 0.006% of ZnSO4·7H2O, 0.009% of FeSO4·7H2O, 0.0005% of CuSO4·5H2O, and 0.001% of MnSO4·4-5H2O was prepared, and 5 ml of the liquid medium was injected into each of large test tubes and then sterilized with steam at 120C for 20 minutes. Then, one platinum loop of the microorganisms of each of the genera shown in Table 1 and 2 was inoculated into the liquid medium, and cultured with shaking at 30C for 2 to 3 days. Cells were collected from each of the culture broths by centrifugation, washed with water and then suspended in 1 ml of a 0.1M phosphate buffer (pH 5.5). Then, 0.5 ml of the cell suspension was mixed with 0.5 ml of a 0.1M KH2PO4 aqueous solution containing 5 mg of 1-chloro-3-hydroxyacetone and 20 mg of glucose, and the resultant mixture was placed in a test tube with a stopper and shaken at 30C for 24 hours. After completion of reaction, a saturated amount of ammonium sulfate was added to the reaction solution, and extraction was performed with an equivalent volume of ethyl acetate. |
With potassium dihydrogenphosphate; D-glucose; In water; at 30℃; for 24h;pH 5.5;Aqueous phophate buffer; Microbiological reaction; | (Example 1) A liquid medium (pH 7.0) containing 4% of glucose, 0.3% of yeast extract, 1.3% of KH2PO4, 0.7% of (NH4)2HPO4, 0.01% of NaCl, 0.08% of MgSO4·7H2O, 0.006% of ZnSO4·7H2O, 0.009% of FeSO4·7H2O, 0.0005% of CuSO4·5H2O, and 0.001% of MnSO4·4-5H2O was prepared, and 5 ml of the liquid medium was injected into each of large test tubes and then sterilized with steam at 120C for 20 minutes. Then, one platinum loop of the microorganisms of each of the genera shown in Table 1 and 2 was inoculated into the liquid medium, and cultured with shaking at 30C for 2 to 3 days. Cells were collected from each of the culture broths by centrifugation, washed with water and then suspended in 1 ml of a 0.1M phosphate buffer (pH 5.5). Then, 0.5 ml of the cell suspension was mixed with 0.5 ml of a 0.1M KH2PO4 aqueous solution containing 5 mg of 1-chloro-3-hydroxyacetone and 20 mg of glucose, and the resultant mixture was placed in a test tube with a stopper and shaken at 30C for 24 hours. After completion of reaction, a saturated amount of ammonium sulfate was added to the reaction solution, and extraction was performed with an equivalent volume of ethyl acetate. | |
With potassium dihydrogenphosphate; D-glucose; In water; at 30℃; for 24h;pH 5.5;Aqueous phophate buffer; Microbiological reaction; | (Example 1) A liquid medium (pH 7.0) containing 4% of glucose, 0.3% of yeast extract, 1.3% of KH2PO4, 0.7% of (NH4)2HPO4, 0.01% of NaCl, 0.08% of MgSO4·7H2O, 0.006% of ZnSO4·7H2O, 0.009% of FeSO4·7H2O, 0.0005% of CuSO4·5H2O, and 0.001% of MnSO4·4-5H2O was prepared, and 5 ml of the liquid medium was injected into each of large test tubes and then sterilized with steam at 120C for 20 minutes. Then, one platinum loop of the microorganisms of each of the genera shown in Table 1 and 2 was inoculated into the liquid medium, and cultured with shaking at 30C for 2 to 3 days. Cells were collected from each of the culture broths by centrifugation, washed with water and then suspended in 1 ml of a 0.1M phosphate buffer (pH 5.5). Then, 0.5 ml of the cell suspension was mixed with 0.5 ml of a 0.1M KH2PO4 aqueous solution containing 5 mg of 1-chloro-3-hydroxyacetone and 20 mg of glucose, and the resultant mixture was placed in a test tube with a stopper and shaken at 30C for 24 hours. After completion of reaction, a saturated amount of ammonium sulfate was added to the reaction solution, and extraction was performed with an equivalent volume of ethyl acetate. | |
With potassium dihydrogenphosphate; D-glucose; In water; at 30℃; for 24h;pH 5.5;Aqueous phophate buffer; Microbiological reaction; | (Example 1) A liquid medium (pH 7.0) containing 4% of glucose, 0.3% of yeast extract, 1.3% of KH2PO4, 0.7% of (NH4)2HPO4, 0.01% of NaCl, 0.08% of MgSO4·7H2O, 0.006% of ZnSO4·7H2O, 0.009% of FeSO4·7H2O, 0.0005% of CuSO4·5H2O, and 0.001% of MnSO4·4-5H2O was prepared, and 5 ml of the liquid medium was injected into each of large test tubes and then sterilized with steam at 120C for 20 minutes. Then, one platinum loop of the microorganisms of each of the genera shown in Table 1 and 2 was inoculated into the liquid medium, and cultured with shaking at 30C for 2 to 3 days. Cells were collected from each of the culture broths by centrifugation, washed with water and then suspended in 1 ml of a 0.1M phosphate buffer (pH 5.5). Then, 0.5 ml of the cell suspension was mixed with 0.5 ml of a 0.1M KH2PO4 aqueous solution containing 5 mg of 1-chloro-3-hydroxyacetone and 20 mg of glucose, and the resultant mixture was placed in a test tube with a stopper and shaken at 30C for 24 hours. After completion of reaction, a saturated amount of ammonium sulfate was added to the reaction solution, and extraction was performed with an equivalent volume of ethyl acetate. | |
With potassium dihydrogenphosphate; D-glucose; In water; at 30℃; for 24h;pH 5.5;Aqueous phophate buffer; Microbiological reaction; | (Example 1) A liquid medium (pH 7.0) containing 4% of glucose, 0.3% of yeast extract, 1.3% of KH2PO4, 0.7% of (NH4)2HPO4, 0.01% of NaCl, 0.08% of MgSO4·7H2O, 0.006% of ZnSO4·7H2O, 0.009% of FeSO4·7H2O, 0.0005% of CuSO4·5H2O, and 0.001% of MnSO4·4-5H2O was prepared, and 5 ml of the liquid medium was injected into each of large test tubes and then sterilized with steam at 120C for 20 minutes. Then, one platinum loop of the microorganisms of each of the genera shown in Table 1 and 2 was inoculated into the liquid medium, and cultured with shaking at 30C for 2 to 3 days. Cells were collected from each of the culture broths by centrifugation, washed with water and then suspended in 1 ml of a 0.1M phosphate buffer (pH 5.5). Then, 0.5 ml of the cell suspension was mixed with 0.5 ml of a 0.1M KH2PO4 aqueous solution containing 5 mg of 1-chloro-3-hydroxyacetone and 20 mg of glucose, and the resultant mixture was placed in a test tube with a stopper and shaken at 30C for 24 hours. After completion of reaction, a saturated amount of ammonium sulfate was added to the reaction solution, and extraction was performed with an equivalent volume of ethyl acetate. | |
With potassium dihydrogenphosphate; D-glucose; In water; at 30℃; for 24h;pH 5.5;Aqueous phophate buffer; Microbiological reaction; | (Example 1) A liquid medium (pH 7.0) containing 4% of glucose, 0.3% of yeast extract, 1.3% of KH2PO4, 0.7% of (NH4)2HPO4, 0.01% of NaCl, 0.08% of MgSO4·7H2O, 0.006% of ZnSO4·7H2O, 0.009% of FeSO4·7H2O, 0.0005% of CuSO4·5H2O, and 0.001% of MnSO4·4-5H2O was prepared, and 5 ml of the liquid medium was injected into each of large test tubes and then sterilized with steam at 120C for 20 minutes. Then, one platinum loop of the microorganisms of each of the genera shown in Table 1 and 2 was inoculated into the liquid medium, and cultured with shaking at 30C for 2 to 3 days. Cells were collected from each of the culture broths by centrifugation, washed with water and then suspended in 1 ml of a 0.1M phosphate buffer (pH 5.5). Then, 0.5 ml of the cell suspension was mixed with 0.5 ml of a 0.1M KH2PO4 aqueous solution containing 5 mg of 1-chloro-3-hydroxyacetone and 20 mg of glucose, and the resultant mixture was placed in a test tube with a stopper and shaken at 30C for 24 hours. After completion of reaction, a saturated amount of ammonium sulfate was added to the reaction solution, and extraction was performed with an equivalent volume of ethyl acetate. | |
With potassium dihydrogenphosphate; D-glucose; In water; at 30℃; for 24h;pH 5.5;Aqueous phophate buffer; Microbiological reaction; | (Example 1) A liquid medium (pH 7.0) containing 4% of glucose, 0.3% of yeast extract, 1.3% of KH2PO4, 0.7% of (NH4)2HPO4, 0.01% of NaCl, 0.08% of MgSO4·7H2O, 0.006% of ZnSO4·7H2O, 0.009% of FeSO4·7H2O, 0.0005% of CuSO4·5H2O, and 0.001% of MnSO4·4-5H2O was prepared, and 5 ml of the liquid medium was injected into each of large test tubes and then sterilized with steam at 120C for 20 minutes. Then, one platinum loop of the microorganisms of each of the genera shown in Table 1 and 2 was inoculated into the liquid medium, and cultured with shaking at 30C for 2 to 3 days. Cells were collected from each of the culture broths by centrifugation, washed with water and then suspended in 1 ml of a 0.1M phosphate buffer (pH 5.5). Then, 0.5 ml of the cell suspension was mixed with 0.5 ml of a 0.1M KH2PO4 aqueous solution containing 5 mg of 1-chloro-3-hydroxyacetone and 20 mg of glucose, and the resultant mixture was placed in a test tube with a stopper and shaken at 30C for 24 hours. After completion of reaction, a saturated amount of ammonium sulfate was added to the reaction solution, and extraction was performed with an equivalent volume of ethyl acetate. | |
With potassium dihydrogenphosphate; D-glucose; In water; at 30℃; for 24h;pH 5.5;Aqueous phophate buffer; Microbiological reaction; | (Example 1) A liquid medium (pH 7.0) containing 4% of glucose, 0.3% of yeast extract, 1.3% of KH2PO4, 0.7% of (NH4)2HPO4, 0.01% of NaCl, 0.08% of MgSO4·7H2O, 0.006% of ZnSO4·7H2O, 0.009% of FeSO4·7H2O, 0.0005% of CuSO4·5H2O, and 0.001% of MnSO4·4-5H2O was prepared, and 5 ml of the liquid medium was injected into each of large test tubes and then sterilized with steam at 120C for 20 minutes. Then, one platinum loop of the microorganisms of each of the genera shown in Table 1 and 2 was inoculated into the liquid medium, and cultured with shaking at 30C for 2 to 3 days. Cells were collected from each of the culture broths by centrifugation, washed with water and then suspended in 1 ml of a 0.1M phosphate buffer (pH 5.5). Then, 0.5 ml of the cell suspension was mixed with 0.5 ml of a 0.1M KH2PO4 aqueous solution containing 5 mg of 1-chloro-3-hydroxyacetone and 20 mg of glucose, and the resultant mixture was placed in a test tube with a stopper and shaken at 30C for 24 hours. After completion of reaction, a saturated amount of ammonium sulfate was added to the reaction solution, and extraction was performed with an equivalent volume of ethyl acetate. | |
With potassium dihydrogenphosphate; D-glucose; In water; at 30℃; for 24h;pH 5.5;Aqueous phophate buffer; Microbiological reaction; | (Example 1) A liquid medium (pH 7.0) containing 4% of glucose, 0.3% of yeast extract, 1.3% of KH2PO4, 0.7% of (NH4)2HPO4, 0.01% of NaCl, 0.08% of MgSO4·7H2O, 0.006% of ZnSO4·7H2O, 0.009% of FeSO4·7H2O, 0.0005% of CuSO4·5H2O, and 0.001% of MnSO4·4-5H2O was prepared, and 5 ml of the liquid medium was injected into each of large test tubes and then sterilized with steam at 120C for 20 minutes. Then, one platinum loop of the microorganisms of each of the genera shown in Table 1 and 2 was inoculated into the liquid medium, and cultured with shaking at 30C for 2 to 3 days. Cells were collected from each of the culture broths by centrifugation, washed with water and then suspended in 1 ml of a 0.1M phosphate buffer (pH 5.5). Then, 0.5 ml of the cell suspension was mixed with 0.5 ml of a 0.1M KH2PO4 aqueous solution containing 5 mg of 1-chloro-3-hydroxyacetone and 20 mg of glucose, and the resultant mixture was placed in a test tube with a stopper and shaken at 30C for 24 hours. After completion of reaction, a saturated amount of ammonium sulfate was added to the reaction solution, and extraction was performed with an equivalent volume of ethyl acetate. | |
With potassium dihydrogenphosphate; D-glucose; In water; at 30℃; for 24h;pH 5.5;Aqueous phophate buffer; Microbiological reaction; | (Example 1) A liquid medium (pH 7.0) containing 4% of glucose, 0.3% of yeast extract, 1.3% of KH2PO4, 0.7% of (NH4)2HPO4, 0.01% of NaCl, 0.08% of MgSO4·7H2O, 0.006% of ZnSO4·7H2O, 0.009% of FeSO4·7H2O, 0.0005% of CuSO4·5H2O, and 0.001% of MnSO4·4-5H2O was prepared, and 5 ml of the liquid medium was injected into each of large test tubes and then sterilized with steam at 120C for 20 minutes. Then, one platinum loop of the microorganisms of each of the genera shown in Table 1 and 2 was inoculated into the liquid medium, and cultured with shaking at 30C for 2 to 3 days. Cells were collected from each of the culture broths by centrifugation, washed with water and then suspended in 1 ml of a 0.1M phosphate buffer (pH 5.5). Then, 0.5 ml of the cell suspension was mixed with 0.5 ml of a 0.1M KH2PO4 aqueous solution containing 5 mg of 1-chloro-3-hydroxyacetone and 20 mg of glucose, and the resultant mixture was placed in a test tube with a stopper and shaken at 30C for 24 hours. After completion of reaction, a saturated amount of ammonium sulfate was added to the reaction solution, and extraction was performed with an equivalent volume of ethyl acetate. | |
With potassium dihydrogenphosphate; D-glucose; In water; at 30℃; for 24h;pH 5.5;Aqueous phophate buffer; Microbiological reaction; | (Example 1) A liquid medium (pH 7.0) containing 4% of glucose, 0.3% of yeast extract, 1.3% of KH2PO4, 0.7% of (NH4)2HPO4, 0.01% of NaCl, 0.08% of MgSO4·7H2O, 0.006% of ZnSO4·7H2O, 0.009% of FeSO4·7H2O, 0.0005% of CuSO4·5H2O, and 0.001% of MnSO4·4-5H2O was prepared, and 5 ml of the liquid medium was injected into each of large test tubes and then sterilized with steam at 120C for 20 minutes. Then, one platinum loop of the microorganisms of each of the genera shown in Table 1 and 2 was inoculated into the liquid medium, and cultured with shaking at 30C for 2 to 3 days. Cells were collected from each of the culture broths by centrifugation, washed with water and then suspended in 1 ml of a 0.1M phosphate buffer (pH 5.5). Then, 0.5 ml of the cell suspension was mixed with 0.5 ml of a 0.1M KH2PO4 aqueous solution containing 5 mg of 1-chloro-3-hydroxyacetone and 20 mg of glucose, and the resultant mixture was placed in a test tube with a stopper and shaken at 30C for 24 hours. After completion of reaction, a saturated amount of ammonium sulfate was added to the reaction solution, and extraction was performed with an equivalent volume of ethyl acetate. | |
With potassium dihydrogenphosphate; D-glucose; In water; at 30℃; for 24h;pH 5.5;Aqueous phophate buffer; Microbiological reaction; | (Example 1) A liquid medium (pH 7.0) containing 4% of glucose, 0.3% of yeast extract, 1.3% of KH2PO4, 0.7% of (NH4)2HPO4, 0.01% of NaCl, 0.08% of MgSO4·7H2O, 0.006% of ZnSO4·7H2O, 0.009% of FeSO4·7H2O, 0.0005% of CuSO4·5H2O, and 0.001% of MnSO4·4-5H2O was prepared, and 5 ml of the liquid medium was injected into each of large test tubes and then sterilized with steam at 120C for 20 minutes. Then, one platinum loop of the microorganisms of each of the genera shown in Table 1 and 2 was inoculated into the liquid medium, and cultured with shaking at 30C for 2 to 3 days. Cells were collected from each of the culture broths by centrifugation, washed with water and then suspended in 1 ml of a 0.1M phosphate buffer (pH 5.5). Then, 0.5 ml of the cell suspension was mixed with 0.5 ml of a 0.1M KH2PO4 aqueous solution containing 5 mg of 1-chloro-3-hydroxyacetone and 20 mg of glucose, and the resultant mixture was placed in a test tube with a stopper and shaken at 30C for 24 hours. After completion of reaction, a saturated amount of ammonium sulfate was added to the reaction solution, and extraction was performed with an equivalent volume of ethyl acetate. | |
With potassium dihydrogenphosphate; D-glucose; In water; at 30℃; for 24h;pH 5.5;Aqueous phophate buffer; Microbiological reaction; | (Example 1) A liquid medium (pH 7.0) containing 4% of glucose, 0.3% of yeast extract, 1.3% of KH2PO4, 0.7% of (NH4)2HPO4, 0.01% of NaCl, 0.08% of MgSO4·7H2O, 0.006% of ZnSO4·7H2O, 0.009% of FeSO4·7H2O, 0.0005% of CuSO4·5H2O, and 0.001% of MnSO4·4-5H2O was prepared, and 5 ml of the liquid medium was injected into each of large test tubes and then sterilized with steam at 120C for 20 minutes. Then, one platinum loop of the microorganisms of each of the genera shown in Table 1 and 2 was inoculated into the liquid medium, and cultured with shaking at 30C for 2 to 3 days. Cells were collected from each of the culture broths by centrifugation, washed with water and then suspended in 1 ml of a 0.1M phosphate buffer (pH 5.5). Then, 0.5 ml of the cell suspension was mixed with 0.5 ml of a 0.1M KH2PO4 aqueous solution containing 5 mg of 1-chloro-3-hydroxyacetone and 20 mg of glucose, and the resultant mixture was placed in a test tube with a stopper and shaken at 30C for 24 hours. After completion of reaction, a saturated amount of ammonium sulfate was added to the reaction solution, and extraction was performed with an equivalent volume of ethyl acetate. | |
With potassium dihydrogenphosphate; D-glucose; In water; at 30℃; for 24h;pH 5.5;Aqueous phophate buffer; Microbiological reaction; | (Example 1) A liquid medium (pH 7.0) containing 4% of glucose, 0.3% of yeast extract, 1.3% of KH2PO4, 0.7% of (NH4)2HPO4, 0.01% of NaCl, 0.08% of MgSO4·7H2O, 0.006% of ZnSO4·7H2O, 0.009% of FeSO4·7H2O, 0.0005% of CuSO4·5H2O, and 0.001% of MnSO4·4-5H2O was prepared, and 5 ml of the liquid medium was injected into each of large test tubes and then sterilized with steam at 120C for 20 minutes. Then, one platinum loop of the microorganisms of each of the genera shown in Table 1 and 2 was inoculated into the liquid medium, and cultured with shaking at 30C for 2 to 3 days. Cells were collected from each of the culture broths by centrifugation, washed with water and then suspended in 1 ml of a 0.1M phosphate buffer (pH 5.5). Then, 0.5 ml of the cell suspension was mixed with 0.5 ml of a 0.1M KH2PO4 aqueous solution containing 5 mg of 1-chloro-3-hydroxyacetone and 20 mg of glucose, and the resultant mixture was placed in a test tube with a stopper and shaken at 30C for 24 hours. After completion of reaction, a saturated amount of ammonium sulfate was added to the reaction solution, and extraction was performed with an equivalent volume of ethyl acetate. | |
With potassium dihydrogenphosphate; D-glucose; In water; at 30℃; for 24h;pH 5.5;Aqueous phophate buffer; Microbiological reaction; | (Example 1) A liquid medium (pH 7.0) containing 4% of glucose, 0.3% of yeast extract, 1.3% of KH2PO4, 0.7% of (NH4)2HPO4, 0.01% of NaCl, 0.08% of MgSO4·7H2O, 0.006% of ZnSO4·7H2O, 0.009% of FeSO4·7H2O, 0.0005% of CuSO4·5H2O, and 0.001% of MnSO4·4-5H2O was prepared, and 5 ml of the liquid medium was injected into each of large test tubes and then sterilized with steam at 120C for 20 minutes. Then, one platinum loop of the microorganisms of each of the genera shown in Table 1 and 2 was inoculated into the liquid medium, and cultured with shaking at 30C for 2 to 3 days. Cells were collected from each of the culture broths by centrifugation, washed with water and then suspended in 1 ml of a 0.1M phosphate buffer (pH 5.5). Then, 0.5 ml of the cell suspension was mixed with 0.5 ml of a 0.1M KH2PO4 aqueous solution containing 5 mg of 1-chloro-3-hydroxyacetone and 20 mg of glucose, and the resultant mixture was placed in a test tube with a stopper and shaken at 30C for 24 hours. After completion of reaction, a saturated amount of ammonium sulfate was added to the reaction solution, and extraction was performed with an equivalent volume of ethyl acetate. | |
With potassium dihydrogenphosphate; D-glucose; In water; at 30℃; for 24h;pH 5.5;Aqueous phophate buffer; Microbiological reaction; | (Example 1) A liquid medium (pH 7.0) containing 4% of glucose, 0.3% of yeast extract, 1.3% of KH2PO4, 0.7% of (NH4)2HPO4, 0.01% of NaCl, 0.08% of MgSO4·7H2O, 0.006% of ZnSO4·7H2O, 0.009% of FeSO4·7H2O, 0.0005% of CuSO4·5H2O, and 0.001% of MnSO4·4-5H2O was prepared, and 5 ml of the liquid medium was injected into each of large test tubes and then sterilized with steam at 120C for 20 minutes. Then, one platinum loop of the microorganisms of each of the genera shown in Table 1 and 2 was inoculated into the liquid medium, and cultured with shaking at 30C for 2 to 3 days. Cells were collected from each of the culture broths by centrifugation, washed with water and then suspended in 1 ml of a 0.1M phosphate buffer (pH 5.5). Then, 0.5 ml of the cell suspension was mixed with 0.5 ml of a 0.1M KH2PO4 aqueous solution containing 5 mg of 1-chloro-3-hydroxyacetone and 20 mg of glucose, and the resultant mixture was placed in a test tube with a stopper and shaken at 30C for 24 hours. After completion of reaction, a saturated amount of ammonium sulfate was added to the reaction solution, and extraction was performed with an equivalent volume of ethyl acetate. | |
With potassium dihydrogenphosphate; D-glucose; In water; at 30℃; for 24h;pH 5.5;Aqueous phophate buffer; Microbiological reaction; | (Example 1) A liquid medium (pH 7.0) containing 4% of glucose, 0.3% of yeast extract, 1.3% of KH2PO4, 0.7% of (NH4)2HPO4, 0.01% of NaCl, 0.08% of MgSO4·7H2O, 0.006% of ZnSO4·7H2O, 0.009% of FeSO4·7H2O, 0.0005% of CuSO4·5H2O, and 0.001% of MnSO4·4-5H2O was prepared, and 5 ml of the liquid medium was injected into each of large test tubes and then sterilized with steam at 120C for 20 minutes. Then, one platinum loop of the microorganisms of each of the genera shown in Table 1 and 2 was inoculated into the liquid medium, and cultured with shaking at 30C for 2 to 3 days. Cells were collected from each of the culture broths by centrifugation, washed with water and then suspended in 1 ml of a 0.1M phosphate buffer (pH 5.5). Then, 0.5 ml of the cell suspension was mixed with 0.5 ml of a 0.1M KH2PO4 aqueous solution containing 5 mg of 1-chloro-3-hydroxyacetone and 20 mg of glucose, and the resultant mixture was placed in a test tube with a stopper and shaken at 30C for 24 hours. After completion of reaction, a saturated amount of ammonium sulfate was added to the reaction solution, and extraction was performed with an equivalent volume of ethyl acetate. | |
With potassium dihydrogenphosphate; D-glucose; In water; at 30℃; for 24h;pH 5.5;Aqueous phophate buffer; Microbiological reaction; | (Example 1) A liquid medium (pH 7.0) containing 4% of glucose, 0.3% of yeast extract, 1.3% of KH2PO4, 0.7% of (NH4)2HPO4, 0.01% of NaCl, 0.08% of MgSO4·7H2O, 0.006% of ZnSO4·7H2O, 0.009% of FeSO4·7H2O, 0.0005% of CuSO4·5H2O, and 0.001% of MnSO4·4-5H2O was prepared, and 5 ml of the liquid medium was injected into each of large test tubes and then sterilized with steam at 120C for 20 minutes. Then, one platinum loop of the microorganisms of each of the genera shown in Table 1 and 2 was inoculated into the liquid medium, and cultured with shaking at 30C for 2 to 3 days. Cells were collected from each of the culture broths by centrifugation, washed with water and then suspended in 1 ml of a 0.1M phosphate buffer (pH 5.5). Then, 0.5 ml of the cell suspension was mixed with 0.5 ml of a 0.1M KH2PO4 aqueous solution containing 5 mg of 1-chloro-3-hydroxyacetone and 20 mg of glucose, and the resultant mixture was placed in a test tube with a stopper and shaken at 30C for 24 hours. After completion of reaction, a saturated amount of ammonium sulfate was added to the reaction solution, and extraction was performed with an equivalent volume of ethyl acetate. | |
With potassium dihydrogenphosphate; D-glucose; In water; at 30℃; for 24h;pH 5.5;Aqueous phophate buffer; Microbiological reaction; | (Example 1) A liquid medium (pH 7.0) containing 4% of glucose, 0.3% of yeast extract, 1.3% of KH2PO4, 0.7% of (NH4)2HPO4, 0.01% of NaCl, 0.08% of MgSO4·7H2O, 0.006% of ZnSO4·7H2O, 0.009% of FeSO4·7H2O, 0.0005% of CuSO4·5H2O, and 0.001% of MnSO4·4-5H2O was prepared, and 5 ml of the liquid medium was injected into each of large test tubes and then sterilized with steam at 120C for 20 minutes. Then, one platinum loop of the microorganisms of each of the genera shown in Table 1 and 2 was inoculated into the liquid medium, and cultured with shaking at 30C for 2 to 3 days. Cells were collected from each of the culture broths by centrifugation, washed with water and then suspended in 1 ml of a 0.1M phosphate buffer (pH 5.5). Then, 0.5 ml of the cell suspension was mixed with 0.5 ml of a 0.1M KH2PO4 aqueous solution containing 5 mg of 1-chloro-3-hydroxyacetone and 20 mg of glucose, and the resultant mixture was placed in a test tube with a stopper and shaken at 30C for 24 hours. After completion of reaction, a saturated amount of ammonium sulfate was added to the reaction solution, and extraction was performed with an equivalent volume of ethyl acetate. | |
With D-glucose; NAD; In water; at 30℃; for 2h;Microbiological reaction; | 5(Example 13) Method for synthesizing (S)-3-chloro1,2-propanediol[0091] Recombinant Escherichia coli HB101 (pNTCRG) Accession No. FERM BP-6898 were inoculated into 50 mlof sterilized 23YT medium (16 g of tryptone, 10 g of yeast extract, 5 g of sodium chloride, and 1L of water, pH 7.0before sterillization) in a 500 ml Sakaguchi flask, and cultured with shaking at 37C for 18 hours. Then, 10 mg of1-chloro-3-hydroxyacetone, 70 mg of NAD+, and 25 mg of glucose were added to 1 ml of the resultant culture broth,and the mixture was then stirred at 30C for 2 hours. After the reaction was completed, the conversion rate and opticalpurity of the reaction product (S)-3-chloro-1,2-propanediol were analyzed by the same method as in Example 9. As aresult, the conversion rate was 42.8%, and the optical purity was 45.8%ee. |