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Chemical Structure| 112392-66-2 Chemical Structure| 112392-66-2

Structure of 112392-66-2

Chemical Structure| 112392-66-2

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Product Details of [ 112392-66-2 ]

CAS No. :112392-66-2
Formula : C9H17NO4
M.W : 203.24
SMILES Code : CC(C(OC)=O)NC(OC(C)(C)C)=O
MDL No. :MFCD01457305

Safety of [ 112392-66-2 ]

GHS Pictogram:
Signal Word:Warning
Hazard Statements:H315-H319
Precautionary Statements:P264-P280-P302+P352-P337+P313-P305+P351+P338-P362+P364-P332+P313

Application In Synthesis of [ 112392-66-2 ]

* All experimental methods are cited from the reference, please refer to the original source for details. We do not guarantee the accuracy of the content in the reference.

  • Downstream synthetic route of [ 112392-66-2 ]

[ 112392-66-2 ] Synthesis Path-Downstream   1~2

  • 1
  • [ 112392-66-2 ]
  • [ 15761-38-3 ]
  • [ 91103-47-8 ]
YieldReaction ConditionsOperation in experiment
With lyophilized cells of Bacillus amyloliquefaciens WZZ002; In aq. phosphate buffer; at 35℃; for 10h;pH 8.0;Enzymatic reaction;Kinetics; Enantioselective hydrolysis was performed on Boc-dl-Ala-OMe by adding both a substrate with a concentration range of 0.1 to 4.0M and a 500mg lyophilized cell of B. amyloliquefaciens WZZ002 in a 10mL (50mL flask) phosphate buffer solution (0.2M, pH6.0-12.0) at 20C to 60C. The solution was stirred at 400rpm. The pH level was controlled through automatic titration using different alkali solutions (2M). The samples were withdrawn at regular intervals and were immediately acidified with HCl (2M) to stop the reaction and to enhance the extractability of Boc-dl-Ala. The sample was extracted using ethyl acetate, whereas the organic phase was isolated and dried using anhydrous Na2SO4 for gas chromatography (GC) analysis. All experiments were conducted in triplicate, unless specified. The time course of enantioselective hydrolysis reaction was performed by adding 2M of Boc-dl-Ala-OMe and 5g of the lyophilized cell of B. amyloliquefaciens WZZ002 in 100mL (250mL flask) phosphate buffer solution (0.2M, pH8.0). The pH of the reaction was controlled through automatic titration using 6M of NH3·H2O to reduce the increasing amount of the neutralizer.
  • 2
  • [ 112392-66-2 ]
  • [ 28875-17-4 ]
  • [ 91103-47-8 ]
YieldReaction ConditionsOperation in experiment
With water; In aq. phosphate buffer; N,N-dimethyl-formamide; at 30℃; for 6h;pH 7.2; General procedure: The soil samples were collected from various locations inChina, and then the strains were screened by utilizing N-Boc-2-aminobutyrate as a sole carbon source and selective platewith bromocresol purple indicator. Isolated strains weretransferred to 150 mL fermentation medium and cultivatedat 200 rpm, 30 C for 48 h. After the cells were harvested, 1 g wet cell were washed and suspended in 5 mL 0.1 M,pH 7.0 potassium phosphate buffer (KPB). Cell suspensionswere mixed with 50 muL substrate solution (N-Boc-2-aminobutyrate:acetone = 1:4,V/V) and kept at 200 rpm, 30 Cfor 6 h reaction, the enantioselective hydrolysis of the substratewas determined by GC-MS. The screening mediumcontained (per liter, pH 7) 0.5 g KCl, 0.5 g MgSO47H2O,1 g K2HPO43H2O, 3 g NaNO3,0.01 g FeSO4,0.25 g Bromocresolpurple, 20 g agar. Fermentation medium contained(per liter, pH 7) 0.5 g KCl, 0.5 g MgSO47H2O, 1 gK2HPO43H2O, 3 g NaNO3,0.01 g FeSO4,20 g sucrose.
 

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