Products Cited in Publications Worldwide Primary Amine-Functionalized Ligand Substitution and Biotinylation of Co (III) for Ligand-Anchored Artificial Metalloenzymes

Primary Amine-Functionalized Ligand Substitution and Biotinylation of Co (III) for Ligand-Anchored Artificial Metalloenzymes

Adams, Milo A; Kincade, Jack A; Runyan, Elle C; Torraco, Ashley N

Abstract

Group IX metals in the+ 3-oxidation state can be incorporated into [Cp* MCl2] 2 complexes for the design and synthesis of new artificial metalloenzymes for biological functions. While previous studies have applied the more costly and less abundant rhodium-or iridium-(III) metals, use of a cobalt (III) metal center provides a low-cost route to preparing artificial metalloenzymes. Herein, a new metal cofactor is acquired through synthesis of a primary amine-functionalized Cp* ligand and subsequent complexation with a Co (III) metal center. Key signals corresponding to the Cp* and NH2 groups are visible by Fourier Transform Infrared spectroscopy where the aromatic signal of the Cp* and the stretching mode of the primary amine are present in their characteristic regions, supporting the formation of a tentatively assigned bis [dichloro-ⴄ5 (1-ethylamine-2, 3, 4, 5-tetramethylcylopentadienyl)-cobalt (III)]. Biotinylation of this complex would allow for pairing with engineered proteins to foster catalytic C—H activation, improving enzymatic processes in biological and biochemical applications.

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Biotin-PFP ester

A276053120550-35-895%

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