Products Cited in Publications Worldwide Combinatorial High-Throughput Screening of Complex Polymeric Enzyme Immobilization Supports

Combinatorial High-Throughput Screening of Complex Polymeric Enzyme Immobilization Supports

Héctor Sánchez-Morán; Joel L. Kaar; Daniel K. Schwartz

DOI:

Abstract

Recent advances have demonstrated the promise of complex multicomponent polymeric supports to enable supra-biological performance. However, the discovery of such supports has been limited by time-consuming, low-throughput synthesis and screening. Here, we describe a novel combinatorial and high-throughput platform that enables rapid screening of complex and heterogeneous copolymer brushes as immobilization supports, named combinatorial high-throughput support screening (CHESS). Using a 384-well plate format, we synthesized arrays of three-component polymer brushes in the microwells using photoactivated surface-initiated polymerization and immobilized in situ. The utility of CHESS to identify optimal immobilization supports under thermally and chemically denaturing conditions was demonstrated usingBacillus subtilisLipase A (LipA). The identification of supports with optimal compositions was validated by immobilizing LipA on polymer-brush-modified biocatalyst particles. We further demonstrated that CHESS could be used to predict the optimal composition of polymer brushes a priori for the previously unexplored , alkaline (AlkP). Our findings demonstrate that CHESS represents a predictable and reliable platform for dramatically accelerating the search of chemical compositions for immobilization supports and further facilitates the discovery of biocompatible and stabilizing materials.

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Tris(2-pyridylmethyl)amine

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