Products Cited in Publications Worldwide Utilizing novel cationic phenylpyridine labeling in glycan profiling by capillary electrophoresis coupled to mass spectrometry

Utilizing novel cationic phenylpyridine labeling in glycan profiling by capillary electrophoresis coupled to mass spectrometry

Smolkova, Denisa; Gregus, Michal; Cmelik, Richard; Pizova, Hana; Jansen-van Vuuren, Ross D; Bobal, Pavel; Lavicka, Jana

DOI:

Abstract

This work focuses on profiling N-linked glycans by capillary electrophoresis coupled to mass spectrometry using a novel fluorescent and mass spectrometry (MS) active derivatization tag. The label is based on 2-phenylpyridine bearing tertiary amine and hydrazide functionalities. It provides efficient labeling via hydrazone formation chemistry, promising fluorescence properties, and ionization efficiency in the positive ion MS mode. Electrophoretic analysis in a neutral-coated capillary allowed baseline separation of maltooligosaccharides with limits of detection in nanomolar concentrations. The developed labeling method was successfully applied to the analyses of N-linked glycans released from several glycoproteins such as bovine ribonuclease B, human immunoglobulin G, or chicken albumin.

Keywords

Oligosaccharides ; Glycans ; Labeling ; Phenylpyridine ; Capillary electrophoresis ; Mass spectrometry

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