Home Products Cited in Publications Worldwide Synthesis and Application of D-and 13C-Labelled tert-Butyl Hoechst Dye
J. Label. Compd. Radiopharm.,2024,67,(12-13):425-430.
Vlasenko, Yulia A; To, Avery J; Fortier, Tess; Evans, Natasha M; Lindsay, Cole J; Palermo, Peter J; Dieckmann, Thorsten; Murphy, Graham K
DOI:10.1002/jlcr.4123 PMID:39441744
Herein, the successful syntheses of D3- and 13C-N-methyl and D9-tert-butyl Hoechst dyes are presented. This includes the preparation of the labelled D3- and 13C-N-methyl piperazines and D9-tert-butylated hydroxytoluene precursors. The tert-butyl Hoechst dye is known to bind a specific RNA aptamer. Spectroscopic NMR studies of the labelled Hoechst dye-aptamer complexes allowed for the unambiguous assignment of chemical shifts, as well as the dynamics of the bound dye.
13C- ; D- ; Hoechst dye ; NMR spectroscopy