Vlasenko, Yulia A; To, Avery J; Fortier, Tess; Evans, Natasha M; Lindsay, Cole J; Palermo, Peter J; Dieckmann, Thorsten; Murphy, Graham K

DOI: PMID:

Abstract

Herein, the successful syntheses of D3- and 13C-N-methyl and D9-tert-butyl Hoechst dyes are presented. This includes the preparation of the labelled D3- and 13C-N-methyl piperazines and D9-tert-butylated hydroxytoluene precursors. The tert-butyl Hoechst dye is known to bind a specific RNA aptamer. Spectroscopic NMR studies of the labelled Hoechst dye-aptamer complexes allowed for the unambiguous assignment of chemical shifts, as well as the dynamics of the bound dye.

Keywords

13C- ; D- ; Hoechst dye ; NMR spectroscopy

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