Home Cart Sign in  
Chemical Structure| 1431697-89-0 Chemical Structure| 1431697-89-0

Structure of OTSSP167
CAS No.: 1431697-89-0

Chemical Structure| 1431697-89-0

*Storage: {[sel_prStorage]}

*Shipping: {[sel_prShipping]}

,{[proInfo.pro_purity]}

OTSSP167 (OTS167) is a highly potent and ATP-competitive MELK inhibitor with an IC50 value of 0.41 nM.

Synonyms: OTS167

4.5 *For Research Use Only !

{[proInfo.pro_purity]}
Cat. No.: {[proInfo.prAm]} Purity: {[proInfo.pro_purity]}

Change View

Size Price VIP Price

US Stock

Global Stock

In Stock
{[ item.pr_size ]} Inquiry {[ getRatePrice(item.pr_usd,item.pr_rate,item.mem_rate,item.pr_is_large_size_no_price, item.vip_usd) ]}

US Stock: ship in 0-1 business day
Global Stock: ship in 5-7 days

  • {[ item.pr_size ]}

In Stock

- +

Please Login or Create an Account to: See VIP prices and availability

US Stock: ship in 0-1 business day
Global Stock: ship in 2 weeks

  • 1-2 Day Shipping
  • High Quality
  • Technical Support
Product Citations

Alternative Products

Product Details of OTSSP167

CAS No. :1431697-89-0
Formula : C25H28Cl2N4O2
M.W : 487.42
SMILES Code : CC(C1=C(N[C@H]2CC[C@H](CN(C)C)CC2)C3=NC(C4=CC(Cl)=C(O)C(Cl)=C4)=CC=C3N=C1)=O
Synonyms :
OTS167
MDL No. :MFCD23160047
InChI Key :DKZYXHCYPUVGAF-UHFFFAOYSA-N
Pubchem ID :135398499

Safety of OTSSP167

GHS Pictogram:
Signal Word:Warning
Hazard Statements:H302-H315-H319-H335
Precautionary Statements:P261-P305+P351+P338

Related Pathways of OTSSP167

PI3K-AKT

Isoform Comparison

Biological Activity

Target
  • MELK

    MELK, IC50:0.41 nM

In Vitro:

Cell Line
Concentration Treated Time Description References
THP-1 50 nM 24 h OTS167 downregulates FLT3 protein expression in FLT3 wild-type AML cell lines. PMC7930094
R7T1 β-cells 5-40 nM 48 hours Assess the induction of human β-cell replication by OTS167 PMC6941846
HEK293T cells 100 nM 48 hours Evaluate the inhibitory effect of OTS167 on DYRK1A PMC6941846
C4-2b cells 30 nM 2 hours To evaluate the inhibitory effect of OTS167 on MELK activity, results showed that OTS167 inhibited phosphorylation of the known MELK substrate ACC at nanomolar concentrations. PMC5840539
Primary human FLT3 mutant AML blast mononuclear cells 50 nM 24 h OTS167 downregulates FLT3 protein expression in primary human FLT3 mutant AML cells. PMC7930094
SK-N-BE2 0 to 8 nM 24 hours To evaluate the effects of OTS167 on cell cycle. Results showed that OTS167 at 8 nM significantly decreased the number of cells in G1 phase and increased the number of cells in S phase. PMC6398941
SW13 0.625 nM, 1.25 nM 24-48 hours To evaluate the effect of OTS167 on SW13 cell proliferation, results showed that OTS167 had antiproliferative and cytotoxic effects at nanomolar concentrations. PMC9502945
Recombinant UGT1A3 2.2 μM (Km) 30 minutes Assessment of UGT1A3 metabolic activity, showing moderate intrinsic clearance (51 ml/min/mg) PMC4468433
Recombinant UGT1A1 5.7 μM (Km) 30 minutes Determination of UGT1A1's metabolic contribution, showing highest intrinsic clearance (64 ml/min/mg) PMC4468433
Human liver microsomes (HLM) 3.4 μM (Km) 30 minutes Identification of glucuronidation as a metabolic pathway for OTS167, observing formation of a single OTS167-glucuronide (OTS167-G) PMC4468433
Recombinant UGT1A10 0.9 μM (Km) 35 minutes Evaluation of UGT1A10 metabolic activity, showing moderate intrinsic clearance (47 ml/min/mg) PMC4468433
Recombinant UGT1A8 3.7 μM (Km) 35 minutes Investigation of intestine-specific UGT1A8's metabolic role, showing highest intrinsic clearance (72 ml/min/mg) PMC4468433
Human intestinal microsomes (HIM) 1.7 μM (Km) 35 minutes Evaluation of intestinal UGTs' contribution to OTS167 metabolism, observing OTS167-G formation PMC4468433
LNCaP cells 30 nM 48 hours To evaluate the effect of OTS167 on apoptosis, results showed that OTS167 increased the fraction of apoptotic cells. PMC5840539
NCI-H295R 1.25 nM, 2.5 nM 48-72 hours To evaluate the effect of OTS167 on NCI-H295R cell proliferation, results showed that OTS167 had antiproliferative and cytotoxic effects at nanomolar concentrations. PMC9502945
PBMC 10 nM 6 days Evaluate the effect of OTS167 on PBMC cells, results showed OTS167 reduced the number of CD138+ cells. PMC5022182
Patient-derived primary ovarian cancer cells 0, 1, 10, 40, 100 nM 72 hours To evaluate the growth inhibitory effect of OTS167 on patient-derived primary ovarian cancer cells, results showed significant growth inhibition at concentrations of 10, 40, and 100 nM. PMC7593222
Ovarian cancer cell lines (ES-2, OVCAR3, OV-90, PA-1, SKOV-3, SW626, CaOV3, RMG-I, OVSAHO, OVTOKO, A2780) 9.3 to 60 nM (IC50) 72 hours To evaluate the cytotoxic effect of OTS167 on ovarian cancer cell lines, results showed significant growth inhibition in all tested cell lines. PMC7593222
KMS-34 CFZ 12.25 nM (IC50) 72 hours Evaluate the effect of OTS167 on KMS-34 CFZ cell viability, results showed OTS167 had comparable effects on the resistant subclone KMS-34 CFZ. PMC5022182
8226 Dox40 60.59 nM (IC50) 72 hours Evaluate the effect of OTS167 on 8226 Dox40 cell viability, results showed 8226 Dox40 cells were the most resistant to OTS167. PMC5022182
MM1R 12.08 nM (IC50) 72 hours Evaluate the effect of OTS167 on MM1R cell viability, results showed OTS167 had comparable effects on the resistant subclone MM1R. PMC5022182
U266 18.47 nM (IC50) 72 hours Evaluate the effect of OTS167 on U266 cell viability, results showed OTS167 decreased cell viability in a dose-dependent manner. PMC5022182
MM1S 7.911 nM (IC50) 72 hours Evaluate the effect of OTS167 on MM1S cell viability, results showed OTS167 decreased cell viability in a dose-dependent manner. PMC5022182
SK-N-DZ 0 to 1 uM 72 hours To evaluate the effects of OTS167 on neuroblastoma cell proliferation. Results showed that OTS167 inhibited the proliferation of neuroblastoma cells with high MELK expression at low nM concentrations. PMC6398941
Ovarian cancer cell lines (ES-2, OVCAR3, OV-90, PA-1, SKOV-3, SW626, CaOV3, RMG-I, OVSAHO, OVTOKO, A2780) 9.3 to 60 nM 72 hours To evaluate the cytotoxic effect of OTS167 on ovarian cancer cell lines, results showed significant growth inhibition in all tested cell lines. PMC7593222
MOLM:14 50 nM 8-24 h OTS167 downregulates FLT3 protein expression in FLT3 mutant AML cell lines. PMC7930094
MV4:11 50 nM 8-24 h OTS167 downregulates FLT3 protein expression in FLT3 mutant AML cell lines. PMC7930094

In Vivo:

Species
Animal Model
Administration Dosage Frequency Description References
Nuþ /Nuþ mice Human ACC xenograft model Intraperitoneal injection 10 mg/kg Daily (Monday-Friday) for five weeks To evaluate the antitumor efficacy of OTS167 in mice with human ACC xenografts, results showed that OTS167 significantly reduced tumor burden. PMC9502945
NOD scid gamma mice C4-2b xenograft model Intraperitoneal injection 10 mg/kg Daily, for the duration of the experiment To evaluate the inhibitory effect of OTS167 on tumor growth, results showed that OTS167 significantly reduced tumor growth and induced apoptosis of tumor cells. PMC5840539
Female nude mice Neuroblastoma xenograft model Intraperitoneal injection 10 mg/kg Twice per week for 3 weeks To evaluate the inhibitory effects of OTS167 on neuroblastoma xenograft growth. Results showed that OTS167 significantly inhibited tumor growth, with the average tumor weight in the treatment group being 77% lower than that in the control group. PMC6398941
NOD.Cg PrkdcscidIL2rgtm1Wjl/SzJ (NSG) mice FLT3 mutant AML xenograft mouse model Oral gavage 5-10 mg/kg 5 days treated/2 days off schedule OTS167 in combination with TKIs results in prolonged survival in a FLT3 mutant AML xenograft mouse model. PMC7930094

Protocol

Bio Calculators
Preparing Stock Solutions 1mg 5mg 10mg

1 mM

5 mM

10 mM

2.05mL

0.41mL

0.21mL

10.26mL

2.05mL

1.03mL

20.52mL

4.10mL

2.05mL

References

 

Historical Records

Categories