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Chemical Structure| 36791-04-5 Chemical Structure| 36791-04-5

Structure of Ribavirin
CAS No.: 36791-04-5

Chemical Structure| 36791-04-5

Ribavirin

CAS No.: 36791-04-5

Ribavirin blocks nucleic acid synthesis and inhibits both RNA and DNA viruses. Ribavirin is a nucleoside antimetabolite antiviral agent.

Synonyms: Ribasphere; NSC 163039; Ribavirin, Copegus, Rebetol, Virazole, ICN-1229

4.5 *For Research Use Only !

Cat. No.: A111785 Purity: 98%

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Product Citations

Product Citations

Kupke, Paul ; Adenugba, Akinbami ; Schemmerer, Mathias ; Bitterer, Florian ; Schlitt, Hans J. ; Geissler, Edward K. , et al.

Abstract: Hepatitis E virus (HEV) is a major cause of acute hepatitis globally. Chronic and fulminant courses are observed especially in immunocompromised transplant recipients since administration of ribavirin (RBV) does not always lead to a sustained virol. response. By in vitro stimulation of NK cells through hepatoma cell lines inoculated with a full-length HEV and treatment with RBV, we analyzed the viral replication and cell response to further elucidate the mechanism of action of RBV on immune cells, especially NK cells, in the context of HEV infection. Co-culture of HEV-infected hepatoma cells with PBMCs and treatment with RBV both resulted in a decrease in viral replication, which in combination showed an additive effect. An anal. of NK cell functions after stimulation revealed evidence of reduced cytotoxicity by decreased TRAIL and CD107a degranulation. Simultaneously, IFN-γ production was significantly increased through the IL-12R pathway. Although there was no direct effect on the IL-12R subunits, downstream events starting with TYK-2 and subsequently pSTAT4 were upregulated. In conclusion, we showed that RBV has an immunomodulatory effect on the IL-12R pathway of NK cells via TYK-2. This subsequently leads to an enhanced IFN-γ response and thus, to an additive antiviral effect in the context of an in vitro HEV infection.

Keywords: ribavirin ; hepatitis E virus ; transplantation ; immunosuppression ; NK cells

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Product Details of Ribavirin

CAS No. :36791-04-5
Formula : C8H12N4O5
M.W : 244.20
SMILES Code : [C@@H]2([N]1N=C(C(N)=O)N=C1)O[C@H](CO)[C@H]([C@H]2O)O
Synonyms :
Ribasphere; NSC 163039; Ribavirin, Copegus, Rebetol, Virazole, ICN-1229
MDL No. :MFCD00058564
InChI Key :IWUCXVSUMQZMFG-AFCXAGJDSA-N
Pubchem ID :37542

Safety of Ribavirin

GHS Pictogram:
Signal Word:Danger
Hazard Statements:H302-H341-H360
Precautionary Statements:P201-P202-P280-P312-P405-P501

Isoform Comparison

Biological Activity

In Vitro:

Cell Line
Concentration Treated Time Description References
Vero E6 cells 50.30 ± 24.01 µM 48 hours To measure the inhibitory effect of Ribavirin on SARS-CoV-2 replication, showing IC50 of 50.30 ± 24.01 μM PMC9872624
Vero E6 cells >500 µM 72 hours To measure the cytotoxicity of Ribavirin on Vero E6 cells, showing CC50 >500 μM PMC9872624
PLC/PRF/5 cells 40 µM 96 hours To evaluate the inhibitory effect of ribavirin monotherapy on HEV growth, results showed that ribavirin monotherapy initially inhibited HEV growth, but the HEV RNA levels gradually increased over time. PMC9697947

Clinical Trial:

NCT Number Conditions Phases Recruitment Completion Date Locations
NCT00216268 Japanese Encephalitis PHASE2 UNKNOWN 2025-05-08 King George Medical University... More >>, Lucknow, UP, 226003, India Less <<
NCT02483260 Viral Hemorrhagic Fever UNKNOWN 2025-12-22 Landstuhl Regional Medical Cen... More >>ter, Landstuhl, APO Ae, 09180, Germany Less <<
NCT00002298 HIV Infections TERMINATED - -
NCT01056757 Metastatic Breast Cancer PHASE1|PHASE2 TERMINATED 2025-11-10 Jewish General Hospital, Montr... More >>eal, Quebec, H3T 1E2, Canada Less <<
NCT00001015 HIV Infections PHASE1 COMPLETED 1990-06-01 Univ of California / San Diego... More >> Treatment Ctr, San Diego, California, 921036325, United States|San Francisco AIDS Clinic / San Francisco Gen Hosp, San Francisco, California, 941102859, United States|Harvard (Massachusetts Gen Hosp), Boston, Massachusetts, 02114, United States|Bellevue Hosp / New York Univ Med Ctr, New York, New York, 10016, United States|Univ of North Carolina, Chapel Hill, North Carolina, 275997215, United States Less <<
NCT00000733 HIV Infections PHASE1 COMPLETED 1991-10-01 Charity Hosp / Tulane Univ Med... More >> School, New Orleans, Louisiana, 70112, United States|Tulane Univ School of Medicine, New Orleans, Louisiana, 70112, United States Less <<
NCT03585725 Follicular Lymphoma|Mantle Cel... More >>l Lymphoma Less << EARLY_PHASE1 TERMINATED 2020-07-22 Weill Cornell Medicine, New Yo... More >>rk, New York, 10065, United States Less <<
NCT03889106 Lassa Fever TERMINATED 2020-10-01 Kenema Government Hospital, Ke... More >>nema, Sierra Leone Less <<
NCT01309490 Malignant Solid Tumour PHASE1|PHASE2 UNKNOWN 2025-06-14 Jewish General Hospital, Montr... More >>eal, Quebec, H3T 1E2, Canada Less <<
NCT00623168 Hemorrhagic Fever With Renal S... More >>yndrome Less << PHASE2 UNKNOWN 2025-12-21 Brian Allgood Army Community H... More >>ospital (121st Combat Support), Seoul, Korea, Republic of Less <<
NCT00001729 Chronic Hepatitis C|Fibrosis|H... More >>emolytic Anemia Less << PHASE3 COMPLETED 2025-09-02 National Institute of Diabetes... More >> and Digestive and Kidney Diseases (NIDDK), Bethesda, Maryland, 20892, United States Less <<
NCT00001854 Chronic Hepatitis C PHASE4 COMPLETED 2008-01-15 National Institutes of Health ... More >>Clinical Center, 9000 Rockville Pike, Bethesda, Maryland, 20892, United States Less <<
NCT00484328 Hepatitis C PHASE4 COMPLETED 2025-12-12 University of Maastricht, Maas... More >>tricht, 6229 ER, Netherlands Less <<
NCT02558114 Hepatitis E PHASE4 TERMINATED 2025-11-18 Hospital Universitari vall d'H... More >>ebron, Barcelona, 08035, Spain Less <<
NCT02308241 Human Papillomavirus (HPV)-Rel... More >>ated Malignancies|Recurrent/Metastatic (R/M) Human Papillomavirus (HPV)-Related Malignancies Less << COMPLETED 2022-03-25 Memorial Sloan Kettering Cance... More >>r Center at Basking Ridge, Basking Ridge, New Jersey, 07939, United States|Memorial Sloan Kettering Monmouth, Middletown, New Jersey, 07748, United States|Memorial Sloan Kettering Cancer Center @ Suffolk, Commack, New York, 11725, United States|Memorial Sloan Kettering Westchester, Harrison, New York, 10604, United States|Memorial Sloan Kettering Cancer Center, New York, New York, 10065, United States|Memorial Sloan Kettering at Mercy Medical Center, Rockville Centre, New York, United States Less <<
NCT00001123 Hantavirus Pulmonary Syndrome COMPLETED 2025-11-05 NIAID/DMID/CASG Central Unit, ... More >>Birmingham, Alabama, 35294, United States Less <<

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