The Hippo pathway does not have specific ligands or receptors. It can be activated by various signals, including cell-cell contact, extracellular matrix (ECM), cell polarity, cell density, and mechanical signals. When the Hippo pathway is activated in healthy cells, MST1/2 and their adaptor protein SAV1 phosphorylate LATS1/2. Once phosphorylated, LATS1/2 and their adaptors MOB1A/B phosphorylate YAP and TAZ. These phosphorylation events lead to the sequestration of YAP and TAZ in the cytoplasm and promote their subsequent degradation. This sequestration and degradation can occur through the binding of YAP and TAZ to 14-3-3 proteins or through the action of the ubiquitin ligase complex βTRCP-SCF. The Hippo pathway regulates organ size control, maintains epithelial cell balance, regulates tissue regeneration, promotes wound healing, and contributes to immune regulation in healthy tissues.