DUE-B is a protein associated with DNA replication initiation and cell cycle regulation. As part of the replication initiation factors, DUE-B is involved in DNA unwinding and the formation of replication forks, essential for cell replication. It is primarily located in the nucleus, working in conjunction with other replication factors like ORC, MCM, and CDT1 to promote DNA unwinding and double-strand stabilization. The expression of DUE-B is tightly regulated throughout the cell cycle, and any dysregulation or genetic mutations can lead to abnormalities in DNA replication, affecting normal cell division and proliferation, and associated with the occurrence of various cancers.