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A mixture of Example 7B (121 mg, 0.3 mmol) and (1-methylpiperidin-2-yl)methanol (39 mg, 0.3 mmol) in anhydrous THF (10 mL) was treated with 1N potassium tert-butoxide in THF (0.3 mL, 0.3 mmol) and the resulting mixture was stirred at room temperature for 1 hour. Acetic acid was added to adjust the acidity to pH 5 and the mixture was concentrated under reduced pressure. A saturated solution of NaHCO3 was then added and the mixture was extracted with ethyl acetate. The organic layer was washed with water, brine, dried with MgSO4, filtered, and concentrated under reduced pressure. The residue was purified by silica gel chromatography and eluted with EtOAc:MeOH (9:1) to afford 110 mg of the title compound. 1H NMR (501 MHz, PYRIDINE-d5) delta ppm 0.88 (t, J=7.3 Hz, 3H), 1.23-1.38 (m, 12H), 1.49 (d, J=12.8 Hz, 1H), 1.59-1.69 (m, 3H), 1.81-1.90 (m, 2H), 1.97-2.05 (m, 1H), 2.18-2.25 (m, 1H), 2.50 (s, 3H), 2.69 (s, 1H), 2.85 (d, J=11.8 Hz, 1H), 4.15 (dd, J=10.0, 5.2 Hz, 1H), 4.41 (t, 2H), 4.50 (dd, J=10.0, 5.0 Hz, 1H), 7.19 (d, J=8.5 Hz, 1H), 7.70 (dd, J=8.6, 2.3 Hz, 1H), 8.53 (d, J=2.4 Hz, 1H); MS (DCI/NH3) m/z 513 (M+H)+. Anal. Calculated for C25H35F3N4O2S.0.1H2O: C, 58.37; H, 6.90; N, 10.89. Found: C, 57.10; H, 7.12; N, 10.54 |