Home Cart Sign in  
HazMat Fee +

There will be a HazMat fee per item when shipping a dangerous goods. The HazMat fee will be charged to your UPS/DHL/FedEx collect account or added to the invoice unless the package is shipped via Ground service. Ship by air in Excepted Quantity (each bottle), which is up to 1g/1mL for class 6.1 packing group I or II, and up to 25g/25ml for all other HazMat items.

Type HazMat fee for 500 gram (Estimated)
Excepted Quantity USD 0.00
Limited Quantity USD 15-60
Inaccessible (Haz class 6.1), Domestic USD 80+
Inaccessible (Haz class 6.1), International USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic USD 100+
Accessible (Haz class 3, 4, 5 or 8), International USD 200+
Chemical Structure| 11089-65-9 Chemical Structure| 11089-65-9
Chemical Structure| 11089-65-9

*Storage: Keep in dark place,Inert atmosphere,Store in freezer, under -20°C.

Tunicamycin

CAS No.: 11089-65-9

Tunicamycin is a homologous nucleoside antibiotic mixture that inhibits N-linked glycosylation and blocks GlcNAc phosphotransferase (GPT), causing ER stress by accumulating unfolded proteins in the endoplasmic reticulum. It induces G1 phase cell cycle arrest, inhibits gram-positive bacteria, yeasts, fungi, and viruses, and has anticancer activity. Additionally, tunicamycin increases exosome release in cervical cancer cells.

Synonyms: Tunicamycin Mixture

4.5 *For Research Use Only !

Cat. No.: A594348 Purity: 99%

Change View

Size Price

USA Stock *0-1 Day

Global Stock *5-7 Days

In Stock
2mg łÇÿď¶ÊÊ In Stock In Stock Login
5mg ł§Çó¶ÊÊ In Stock In Stock Login
10mg łÿ§ď¶ÊÊ In Stock In Stock Login
25mg łîËî¶ÊÊ Inquiry In Stock Login
50mg łÇ§îͶÊÊ Inquiry Inquiry Login

Please Login or Create an Account to: See VIP prices and availability

  • 2mg

    łÇÿď¶ÊÊ

  • 5mg

    ł§Çó¶ÊÊ

  • 10mg

    łÿ§ď¶ÊÊ

  • 25mg

    łîËî¶ÊÊ

  • 50mg

    łÇ§îͶÊÊ

In Stock

- +

Please Login or Create an Account to: See VIP prices and availability

  • 1-2 Day Shipping
  • High Quality
  • Technical Support Online Technical Q&A
Product Citations

Product Details of [ Tunicamycin ]

CAS No. :11089-65-9
Formula : C37H60N4O16
M.W : 816.89
SMILES Code : NONE
Synonyms :
Tunicamycin Mixture
MDL No. :MFCD00065709

Safety of [ Tunicamycin ]

GHS Pictogram:
Signal Word:Danger
Hazard Statements:H300
Precautionary Statements:P264-P301+P310
Class:6.1
UN#:3462
Packing Group:

Biological Activity

In Vitro:

Cell Line
Concentration Treated Time Description Reference
HEK-293T cells 1 µM 24 h To detect abnormal N-glycosylation, it was found that inhibition of N-glycosylation stabilized the localization of Halo protein in the ER PMC7810369
A549 cells 1 µM 24 h Induction of Halo1N fluorescence by inhibition of N-glycosylation validated Halo1N as a reliable indicator of abnormal N-glycosylation PMC7810369
MC38 epithelial cells 1μM 4 h Induce ER stress and promote AIEC MSL1 replication PMC9805753
SH-SY5Y neuroblastoma cells 1 μg/ml 48 h To investigate the effect of Tunicamycin on ER stress-induced cell death in SH-SY5Y cells and found that flurbiprofen significantly inhibited this cell death. PMC3958308
NIT-1 pancreatic β-cells 0.05 μg/mL 24 h To investigate the effect of Tunicamycin on the viability of NIT-1 pancreatic β-cells, the results showed that Tunicamycin significantly decreased cell viability in a dose-dependent manner. PMC8229938
HepG2 cells 12 μM 6 h Induction of ER stress, suppression of CYP7A1 expression, and increased expression of ABCB11 and ABCC3 PMC5331781
CHO cells 500nM 24 h To validate the function of the NLG reporter, showing increased bioluminescence in CHO cells after Tunicamycin treatment PMC3413408
293T cells 500nM To validate the design of the NLG reporter, showing altered glycosylation status of Luc protein in 293T cells after Tunicamycin treatment PMC3413408
Huh7shCON and Huh7shXBP1 cells 5 μg/ml 6 h To induce ER stress and investigate the role of XBP1 in ER stress-induced FGF21 expression. Results showed that despite XBP1 knockdown, Huh7shXBP1 cells could still induce FGF21 expression normally under ER stress. PMC5699917
MEF cells 3 μg/ml 48 h To investigate the change of EVA1A expression under ER stress, the results showed a significant increase of EVA1A protein level in the wild type (WT) cells, but not in Chop knockout cells upon tunicamycin treatment. PMC9887066
HEK293 cells 3 μg/ml 48 h To investigate the interaction between EVA1A and MCL1, the results showed a significant interaction of EVA1A with MCL1 in cells after tunicamycin treatment. PMC9887066
LSK cells 3 μg/ml 36 h To investigate the change of EVA1A expression under ER stress, the results showed an enhanced EVA1A mRNA level in the LSK cells from the mice treated with tunicamycin. PMC9887066
Caco-2 cells 2 µg/ml 5 or 7 h To study the effect of ER stress on HMGCS2 expression, results showed that TM significantly decreased HMGCS2 transcription PMC10348483
HT29 cells 2 µg/ml 5 or 7 h To study the effect of ER stress on HMGCS2 expression, results showed that TM significantly decreased HMGCS2 transcription PMC10348483

In Vivo:

Species
Animal Model
Administration Dosage Frequency Description Reference
C57BL/6J mice ER stress model Intraperitoneal injection 0.5 mg/kg (acute), 0.1 mg/kg (chronic) Single injection (acute), daily injections for 5 days (chronic) Induction of ER stress, suppression of Cyp7a1 expression, increased expression of Abcb11 and Abcc3, and reduced hepatic bile acid content PMC5331781
Nude mice D54 glioma xenograft model Intraperitoneal injection 0.75mg/kg Single dose, lasting several days To evaluate the effect of Tunicamycin on NLG inhibition in vivo, showing that Tunicamycin significantly reduces EGFR and Met protein levels in D54 tumors and enhances tumor radiosensitivity PMC3413408
mice hepatocyte-specific Xbp1 knockout mice (Xbp1LKO) intraperitoneal injection 0.5 mg/kg single injection, sacrificed 6 hours post-injection To investigate the induction of FGF21 in hepatocyte-specific Xbp1 knockout mice under pharmacologic ER stress. Results showed that Xbp1LKO mice could still induce FGF21 expression normally under ER stress, indicating that Xbp1 is not required for ER stress-induced FGF21 expression. PMC5699917
mice WT and Eva1aF/F,Vav-Cre mice intraperitoneal injection 0.5 mg/kg every 3 days for 7 days To investigate the change of EVA1A expression under ER stress and its effect on HSPCs, the results showed that Eva1a deficient mice had more LSK, LT-HSC, ST-HSC, MPP3, MPP4, CMP, and GMP cells in BM than that in WT mice after tunicamycin treatment. PMC9887066
C57BL/6 mice Wild type and Xbp1DIEC mice Intraperitoneal injection 10 µg/gr Single injection, sacrificed at 24 hours To study the effect of ER stress on HMGCS2 expression, results showed that TM significantly decreased HMGCS2 expression PMC10348483

Clinical Trial:

NCT Number Conditions Phases Recruitment Completion Date Locations
NCT01754441 - Unknown December 2017 United States, Delaware ... More >> Alfred I. duPont Hospital for Children Wilmington, Delaware, United States, 19803 United States, Florida Nemours Children's Specialty Care, Jacksonville Jacksonville, Florida, United States, 32207 Nemours Children's Hospital Orlando, Florida, United States, 32827 Less <<

Protocol

Bio Calculators
Preparing Stock Solutions 1mg 5mg 10mg

1 mM

5 mM

10 mM

1.22mL

0.24mL

0.12mL

6.12mL

1.22mL

0.61mL

12.24mL

2.45mL

1.22mL

Dissolving Methods
Please choose the appropriate dissolution scheme according to your animal administration guide.For the following dissolution schemes, clear stock solution should be prepared according to in vitro experiments, and then cosolvent should be added in turn:

in order to ensure the reliability of the experimental results, the clarified stock solution can be properly preserved according to the storage conditions; The working fluid for in vivo experiment is recommended to be prepared now and used on the same day;

The percentage shown in front of the following solvent refers to the volume ratio of the solvent in the final solution; If precipitation or precipitation occurs in the preparation process, it can be assisted by heating and/or ultrasound.
Protocol 1
Protocol 2

References

 

Historical Records