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Chemical Structure| 10045-45-1 Chemical Structure| 10045-45-1
Chemical Structure| 10045-45-1

1-EBIO

CAS No.: 10045-45-1

1-EBIO is an epithelial K+–Ca2+ channel activator that stimulates sustained transepithelial Cl- secretion in T84 cell monolayers, induces hyperpolarization of aortic valve endothelial cells, and promotes differentiation of embryonic stem cells into cardiomyocytes.

Synonyms: 1-Ethyl-2-benzimidazolinone;1-Ethylbenzimidazolinone

4.5 *For Research Use Only !

Cat. No.: A147983 Purity: 97%

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Product Details of [ 1-EBIO ]

CAS No. :10045-45-1
Formula : C9H10N2O
M.W : 162.19
SMILES Code : O=C1N(CC)C2=CC=CC=C2N1
Synonyms :
1-Ethyl-2-benzimidazolinone;1-Ethylbenzimidazolinone
MDL No. :MFCD00005715
InChI Key :CXUCKELNYMZTRT-UHFFFAOYSA-N
Pubchem ID :82320

Safety of [ 1-EBIO ]

GHS Pictogram:
Signal Word:Warning
Hazard Statements:H302-H319
Precautionary Statements:P305+P351+P338

Protocol

Bio Calculators
Preparing Stock Solutions 1mg 5mg 10mg

1 mM

5 mM

10 mM

6.17mL

1.23mL

0.62mL

30.83mL

6.17mL

3.08mL

61.66mL

12.33mL

6.17mL

Dissolving Methods
Please choose the appropriate dissolution scheme according to your animal administration guide.For the following dissolution schemes, clear stock solution should be prepared according to in vitro experiments, and then cosolvent should be added in turn:

in order to ensure the reliability of the experimental results, the clarified stock solution can be properly preserved according to the storage conditions; The working fluid for in vivo experiment is recommended to be prepared now and used on the same day;

The percentage shown in front of the following solvent refers to the volume ratio of the solvent in the final solution; If precipitation or precipitation occurs in the preparation process, it can be assisted by heating and/or ultrasound.
Protocol 1
Protocol 2

References

[1]References are publications that support the biological activity of the product. Ayotunde and Adeagbo (1999) 1-Ethyl-2-benzimidazolinone stimulates endothelial KCa channels and nitric oxide formation in rat mesenteric vessels. Eur.J.Pharmacol. 379 151 PMID: 10497901 Devor et al (1996) Modulation of Cl- secretion by benzimidazolones. I. Direct activation of a Ca2+ - dependent K+ channel. Am.J.Physiol. 271 L775 PMID: 8944721 Kusama et al (2005) Reduced hyperpolarisation in endothelial cells of rabbit aortic value following chronic nitroglycerine administration. Br.J.Pharmacol. 146 487 PMID: 16056232 Kleger et al (2010) Modulation of calcium-activated potassium channels induces cardiogenesis of pluripotent stem cells and enrichment of pacemaker-like cells. Circulation. 122 1823 PMID: 20956206 If you know of a relevant reference for 1-EBIO, please let us know.

[2]Roth EK, Hirtz S, Duerr J, Wenning D, Eichler I, Seydewitz HH, Amaral MD, Mall MA. The K+ channel opener 1-EBIO potentiates residual function of mutant CFTR in rectal biopsies from cystic fibrosis patients. PLoS One. 2011;6(8):e24445

[3]Yin MZ, Park SW, Kang TW, Kim KS, Yoo HY, Lee J, Hah JH, Sung MH, Kim SJ. Activation of K(+) channel by 1-EBIO rescues the head and neck squamous cell carcinoma cells from Ca(2+) ionophore-induced cell death. Korean J Physiol Pharmacol. 2016 Jan;20(1):25-33

[4]Alvarez LJ, Zamudio AC, Candia OA. Cl- secretory effects of EBIO in the rabbit conjunctival epithelium. Am J Physiol Cell Physiol. 2005 Jul;289(1):C138-47

[5]Edwards G, Gardener MJ, Feletou M, Brady G, Vanhoutte PM, Weston AH. Further investigation of endothelium-derived hyperpolarizing factor (EDHF) in rat hepatic artery: studies using 1-EBIO and ouabain. Br J Pharmacol. 1999 Nov;128(5):1064-70

 

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