Alternatived Products of [ 634-74-2 ]
Product Details of [ 634-74-2 ]
CAS No. : | 634-74-2 |
MDL No. : | MFCD11978165 |
Formula : |
C6H12O5
|
Boiling Point : |
- |
Linear Structure Formula : | - |
InChI Key : | PNNNRSAQSRJVSB-KVTDHHQDSA-N |
M.W : |
164.16
|
Pubchem ID : | 5460029 |
Synonyms : |
|
Application In Synthesis of [ 634-74-2 ]
* All experimental methods are cited from the reference, please refer to the original source for details. We do not guarantee the accuracy of the content in the reference.
- Downstream synthetic route of [ 634-74-2 ]
- 1
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[ 634-74-2 ]
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[ 3615-37-0 ]
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[ 2438-80-4 ]
- 2
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polysaccharide from Artemisia argyi, MW 5169 Da
[ No CAS ]
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[ 58-86-6 ]
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[ 3458-28-4 ]
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[ 10323-20-3 ]
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[ 50-69-1 ]
-
[ 634-74-2 ]
-
[ 50-99-7 ]
-
[ 59-23-4 ]
-
[ 7512-17-6 ]
Yield | Reaction Conditions | Operation in experiment |
|
With trifluoroacetic acid; In water; at 100℃; for 8h;Sealed tube; |
A simple and sensitive high performance liquid chromatographic method was applied to the simultaneous determination of nine kinds of monosaccharides (glucose, rhamnose, mannose, arabinose, galactose, xylose, ribose, galacturonic acid and N-acetyl-d-glucosamine) in FAAP-02 by pre-column derivatization with 1-phenyl-3-5-pyrazolone (PMP) as described previously with proper modification (Lv et al., 2009). In brief, FAAP-02 was hydrolyzed with TFA (3M) for 8h at 100C in a sealed ampoule. After cooling to room temperature, the reaction mixture was centrifuged and the supernatant was collected and lyophilized. The hydrolyzed sample or monosaccharide standard aqueous solution was mixed with aqueous NaOH (0.25M) and PMP methanol solution (0.25M) thoroughly. Then each mixture was incubated at 70C for 90min. After cooled and neutralized with HCl (0.25M), the resulting solution was extracted with chloroform to remove the excess reagents. The aqueous layer was filtered through a 0.45mum membrane before detection. The analysis of PMP-labeled monosaccharides was performed on an Agilent 1260 HPLC system equipped with a ZORBAX Eclipse XDB-C18 (250mm×4.6mm, id: 5mum, column temperature: 30C). The wavelength of detection was 250nm. Elution was carried out with a mixture of acetonitrile and phosphate buffer (0.06M, pH 6.8) in a ratio of 17: 83 (v/v, %) at a flow rate of 1mL/min. |