[ CAS No. 35542-01-9 ] {[proInfo.proName]}

Name: 35542-01-9|1-((2R,3R,4S,5R)-3,4-Dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methoxypyrimidine-2,4(1H,3H)-dione|95%
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SKU: A412544
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Quality Control of [ 35542-01-9 ]

COA NMR CNMR HPLC LC-MS

Related Doc. of [ 35542-01-9 ]

SDS Specification

Alternatived Products of [ 35542-01-9 ]

Product Details of [ 35542-01-9 ]

CAS No. :	35542-01-9	MDL No. :	MFCD00006533
Formula :	C₁₀H₁₄N₂O₇	Boiling Point :	-
Linear Structure Formula :	-	InChI Key :	ZXIATBNUWJBBGT-JXOAFFINSA-N
M.W :	274.23	Pubchem ID :	1265899
Synonyms :		Chemical Name :	1-((2R,3R,4S,5R)-3,4-Dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methoxypyrimidine-2,4(1H,3H)-dione

Calculated chemistry of [ 35542-01-9 ]

Physicochemical Properties

Num. heavy atoms :	19
Num. arom. heavy atoms :	6
Fraction Csp3 :	0.6
Num. rotatable bonds :	3
Num. H-bond acceptors :	7.0
Num. H-bond donors :	4.0
Molar Refractivity :	60.76
TPSA :	134.01 Å²

Pharmacokinetics

GI absorption :	Low
BBB permeant :	No
P-gp substrate :	No
CYP1A2 inhibitor :	No
CYP2C19 inhibitor :	No
CYP2C9 inhibitor :	No
CYP2D6 inhibitor :	No
CYP3A4 inhibitor :	No
Log Kp (skin permeation) :	-9.76 cm/s

Lipophilicity

Log Po/w (iLOGP) :	0.48
Log Po/w (XLOGP3) :	-2.52
Log Po/w (WLOGP) :	-3.17
Log Po/w (MLOGP) :	-2.45
Log Po/w (SILICOS-IT) :	-1.37
Consensus Log Po/w :	-1.8

Druglikeness

Lipinski :	0.0
Ghose :	None
Veber :	0.0
Egan :	1.0
Muegge :	1.0
Bioavailability Score :	0.55

Water Solubility

Log S (ESOL) :	0.01
Solubility :	282.0 mg/ml ; 1.03 mol/l
Class :	Highly soluble
Log S (Ali) :	0.25
Solubility :	487.0 mg/ml ; 1.78 mol/l
Class :	Highly soluble
Log S (SILICOS-IT) :	0.62
Solubility :	1150.0 mg/ml ; 4.2 mol/l
Class :	Soluble

Medicinal Chemistry

PAINS :	0.0 alert
Brenk :	0.0 alert
Leadlikeness :	0.0
Synthetic accessibility :	4.13

Safety of [ 35542-01-9 ]

Signal Word:	Warning	Class:	N/A
Precautionary Statements:	P261-P305+P351+P338	UN#:	N/A
Hazard Statements:	H315-H319-H335	Packing Group:	N/A
GHS Pictogram:

Application In Synthesis of [ 35542-01-9 ]

* All experimental methods are cited from the reference, please refer to the original source for details. We do not guarantee the accuracy of the content in the reference.

Downstream synthetic route of [ 35542-01-9 ]

[ 35542-01-9 ] Synthesis Path-Downstream 1~29

1
[ 35542-01-9 ]
[ 37805-91-7 ]

Yield	Reaction Conditions	Operation in experiment
	With bis(phenyl) carbonate; sodium hydrogencarbonate In N,N-dimethyl-formamide at 150℃; for 0.5h;
	With bis(phenyl) carbonate; sodium hydrogencarbonate	C. Representative Procedure for Ring Closure Reactions Method B: The ribonucleoside (1.0 equiv.) was dissolved in anhydrous dimethylformamide (100 μL per mmol). Diphenylcarbonate (1.0 equiv.) and sodium bicarbonate (0.05 equiv.) were added and the mixture was stirred at 100° C. for 8-12 hours. Ether was added to precipitate the product as a gum. The solvent was decanted and the crude residue was purified by either flash column chromatography or preparative HPLC

Reference： [1]Stout,M.G.; Robins,R.K. [Journal of Heterocyclic Chemistry, 1972, vol. 9, p. 545 - 549]
[2]Current Patent Assignee: TOSK - US9382287, 2016, B2 Location in patent: Page/Page column 44; 45

Yield	Reaction Conditions	Operation in experiment
	Uracil (2), Na-methoxid;
		The synthetic mRNA construct of claim 1, wherein the mRNA construct comprises one or more chemically-modified nucleotides selected from the group of 5-hydroxyuridine, 5-methyluridine, 5,6-dihydro-5-methyluridine, 2'-O-methyluridine, 2'-O-methyl-5-methyluridine, 2'-fluoro-2'-deoxyuridine, 2'-amino-2'-deoxyuridine, 2'-azido-2'-deoxyuridine, 4-thiouridine, 5-hydroxymethyluridine, 5-carboxyuridine, 5-carboxymethylesteruridine, 5-formyluridine, 5-methoxyuridine, 5-propynyluridine, 5-bromouridine, 5-iodouridine, 5-fluorouridine;pseudouridine, 2'-O-methyl-pseudouridine, N1-hydroxypseudouridine, N1-methylpseudouridine, 2'-O-methyl-N1-methylpseudouridine, N1-ethylpseudouridine, N1-hydroxymethylpseudouridine, and Arauridine;5-hydroxycytidine, 5-methylcytidine, 5-hydroxymethylcytidine, 5-carboxycytidine, 5-formylcytidine, 5-methoxycytidine, 5-propynylcytidine, 2-thiocytidine;5-hydroxyuridine, 5-methyluridine, 5,6-dihydro-5-methyluridine, 2'-O-methyluridine, 2'-O-methyl-5-methyluridine, 2'-fluoro-2'-deoxyuridine, 2'-amino-2'-deoxyuridine, 2'-azido-2'-deoxyuridine, 4-thiouridine, 5-hydroxymethyluridine, 5-carboxyuridine, 5-carboxymethylesteruridine, 5-formyluridine, 5-methoxyuridine, 5-propynyluridine, 5-bromouridine, 5-iodouridine, 5-fluorouridine;N6-methyladenosine, 2-aminoadenosine, 3-methyladenosine, 7-deazaadenosine, 8-oxoadenosine, inosine;thienoguanosine, 7-deazaguanosine, 8-oxoguanosine, and 6-O-methylguanine.

3
[ 35542-01-9 ]
C₁₀H₁₃Cl₂N₂O₈P [ No CAS ]

Yield	Reaction Conditions	Operation in experiment
	With trimethyl phosphite; N,N,N',N'-tetramethyl-1,8-diaminonaphthalene; trichlorophosphate at -15℃; Inert atmosphere;
	Stage #1: 5-methoxycarbonylmethyl-2'-O-methyl-uridine With trimethyl phosphite; N,N,N',N'-tetramethyl-1,8-diaminonaphthalene at 0℃; for 0.166667h; Stage #2: With trichlorophosphate for 2h; Inert atmosphere;	32 Example 32. Synthesis of 5-methoxy uridine (compound 15) and 5-methoxy UTP (NTP of said compound) Example 32. Synthesis of 5-methoxy uridine (compound 15) and 5-methoxy UTP (NTP of said compound) A solution of 5-methoxy uridine (compound 15) (69.0 mg, 0.25 mmol, plus heat to make it soluble) was added to proton sponge (80.36 mg, 0.375 mmol, 1.50 equiv.) in 0.7 mL trimethylphosphate (TMP) and was stirred for 10 minutes at 0°C. Phosphorous oxychloride (POCl3) (46.7 ul, 0.50 mmol, 2.0 equiv.) was added dropwise to the solution before being kept stirring for 2 hours under N2 atmosphere. After 2 hours the solution was reacted with a mixture of bistributylammonium pyrophosphate (TBAPP or (n-Bu3NH)2H2P2O7) (894.60 mg, 1.63 mmol, 6.50 equiv.) and tributylamine (243.0 ul, 1.00 mmol, 4.0 equiv.) in 2.0 ml of dimethylformamide. After approximately 15 minutes, the reaction was quenched with 17.0 ml of 0.2M triethylammonium bicarbonate (TEAB) and the clear solution was stirred at room temperature for an hour. The reaction mixture was lyophilized overnight and the crude reaction mixture was purified by HPLC (Shimadzu, Kyoto Japan, Phenomenex C18 preparative column, 250 x 21.20 mm, 10.0 micron; gradient: 100 % A for 3.0 min, then 1% B/min, A = 100 mM TEAB buffer, B = ACN; flow rate: 10.0 mL/min; retention time: 16.57-17.51 min). Fractions containing the desired compound were pooled and lyophilized to produce the NTP of compound 15. The triphosphorylation reactions were carried out in a two-neck flask flame-dried under N2 atmosphere. Nucleosides and the protein sponge were dried over P2O5 under vacuum overnight prior to use. The formation of monophosphates was monitored by LCMS

Reference： [1]Location in patent: experimental part Ginsburg-Shmuel, Tamar; Haas, Michael; Schumann, Marien; Reiser, Georg; Kalid, Ori; Stern, Noa; Fischer, Bilha [Journal of Medicinal Chemistry, 2010, vol. 53, # 4, p. 1673 - 1685]
[2]Current Patent Assignee: MODERNA THERAPEUTICS INC - EP2918275, 2015, A1 Location in patent: Paragraph 2089; 2090

4
[ 35542-01-9 ]
5-OMe-uridine-5'-O-(α-boranodiphosphate) [ No CAS ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 2 steps 1.1: toluene-4-sulfonic acid / 24 h / 20 °C / Inert atmosphere 1.2: dowex 2.1: 2-Chloro-4H-1,3,2-benzodioxaphosphorin-4-one / N,N-dimethyl-formamide; 1,4-dioxane; pyridine / 0.17 h / 20 °C / Inert atmosphere 2.2: 0.25 h / 20 °C
	Multi-step reaction with 3 steps 1.1: toluene-4-sulfonic acid / 24 h / 20 °C / Inert atmosphere 1.2: dowex 2.1: 2-Chloro-4H-1,3,2-benzodioxaphosphorin-4-one / N,N-dimethyl-formamide; 1,4-dioxane; pyridine / 0.17 h / 20 °C / Inert atmosphere 2.2: 0.08 h / 20 °C 2.3: 0.25 h / 20 °C 3.1: water / hydrogenchloride / 3 h / 20 °C / pH 2.3 3.2: 0.75 h / 20 °C / pH 9

Reference： [1]Current Patent Assignee: COMPLUTENSE UNIVERSITY OF MADRID; BAR-ILAN UNIVERSITY - WO2012/73237, 2012, A1
[2]Current Patent Assignee: COMPLUTENSE UNIVERSITY OF MADRID; BAR-ILAN UNIVERSITY - WO2012/73237, 2012, A1

5
[ 35542-01-9 ]
C₁₂H₁₈BN₂O₁₃P₂^(3-) [ No CAS ]
C₁₂H₁₈BN₂O₁₆P₃^(4-) [ No CAS ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 2 steps 1.1: toluene-4-sulfonic acid / 24 h / 20 °C / Inert atmosphere 1.2: dowex 2.1: 2-Chloro-4H-1,3,2-benzodioxaphosphorin-4-one / N,N-dimethyl-formamide; 1,4-dioxane; pyridine / 0.17 h / 20 °C / Inert atmosphere 2.2: 0.08 h / 20 °C 2.3: 0.25 h / 20 °C

Reference： [1]Current Patent Assignee: COMPLUTENSE UNIVERSITY OF MADRID; BAR-ILAN UNIVERSITY - WO2012/73237, 2012, A1

6
[ 35542-01-9 ]
[ 149-73-5 ]
2′,3′-O-methoxymethylidene-5-OMe-uridine [ No CAS ]

Yield	Reaction Conditions	Operation in experiment
96.3%	Stage #1: 5-methoxycarbonylmethyl-2'-O-methyl-uridine; trimethyl orthoformate With toluene-4-sulfonic acid at 20℃; for 24h; Stage #2: at 20℃; for 3h;	2.2 2′,3′-O-Methoxymethylidene-5-OMe-uridine (25) A suspension of 5-OMe-uridine, 24, (300 mg, 1.09 mmol) and p-TsOH (catalytic amount) in trimethyl orthoformate (1.09 mL, 9.85 mmol, 9 equiv) was prepared in a flamed-dried, nitrogen-flushed two-necked round bottom flask, and stirred at rt. After 24 h the solution became almost clear and TLC (CHCl3:MeOH 8:2) showed two less polar spots and the complete disappearance of the starting material. Dowex (weak base) was added (0.31 g, 1.09 mmol, 1 equiv) and the mixture was stirred at rt for 3 h. The liquid was decanted and the MeOH was used for washes. The solution was evaporated to give an oil-like residue. Co evaporations with ether were done and a white solid was obtained (a mixture of two diastereomers: 331.8 mg, 96.3%).
	Stage #1: 5-methoxycarbonylmethyl-2'-O-methyl-uridine; trimethyl orthoformate at 20℃; for 24h; Inert atmosphere; Stage #2: With dowex	1 Synthesis of 2 ',3 '-O-methoxymethylidene-5-OMe-uridine, 2[0070] The protected nucleoside, 2, was synthesized as previously described (Griffin et al , 1967). In particular, a suspension of 5-OMe-uridine, 1 , (300 mg, 1.09 mmol) and p-TsOH (catalytic amount) in trimethyl orthoformate ( 1 .09 ml, 9.85 mmol, 9 eq) was prepared in a flamed-dried, nitrogen-flushed two-necked round bottom flask, and stirred at room temperature (RT). After 24 h, the solution became almost clear and TLC (CHCl3:MeOH 8:2) showed two less polar spots and the complete disappearance of the starting material. Dowex (weak base) was added (0.3 1 gr, 1 .09 mmol, 1 eq) and the mixture was stirred at RT for 3 h. The liquid was decanted and the MeOH was used for washes. The solution was evaporated to give an oil-like residue. Co evaporations with ether were done and a white solid was obtained (a mixture of two diastereomers: 33 1 .8 mg, 96.3%).

Reference： [1]Ginsburg-Shmuel, Tamar; Haas, Michael; Grbic, Djordje; Arguin, Guillaume; Nadel, Yael; Gendron, Fernand-Pierre; Reiser, Georg; Fischer, Bilha [Bioorganic and Medicinal Chemistry, 2012, vol. 20, # 18, p. 5483 - 5495]
[2]Current Patent Assignee: COMPLUTENSE UNIVERSITY OF MADRID; BAR-ILAN UNIVERSITY - WO2012/73237, 2012, A1 Location in patent: Page/Page column 22-23

7
[ 35542-01-9 ]
C₁₀H₁₆BN₂O₁₅P₃^(4-)*4Na⁽¹⁺⁾ [ No CAS ]
C₁₀H₁₆BN₂O₁₂P₂^(3-)*3Na⁽¹⁺⁾ [ No CAS ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 5 steps 1.1: toluene-4-sulfonic acid / 24 h / 20 °C 1.2: Dowex / 3 h / 20 °C 2.1: pyridine / N,N-dimethyl-formamide; 1,4-dioxane / 0.17 h / 20 °C 3.1: tributyl-amine / N,N-dimethyl-formamide; 1,4-dioxane / 0.08 h / 20 °C 4.1: dimethylsulfide borane complex / N,N-dimethyl-formamide; 1,4-dioxane; tetrahydrofuran / 0.25 h / 20 °C 5.1: ethylenediamine / N,N-dimethyl-formamide; 1,4-dioxane; tetrahydrofuran / 0.17 h / 20 °C 5.2: 3 h / 20 °C / pH 2.3

8
[ 35542-01-9 ]
2′,3′-O-methoxymethylidene-5-OMe-uridine [ No CAS ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 2 steps 1.1: toluene-4-sulfonic acid / 24 h / 20 °C 1.2: Dowex / 3 h / 20 °C 2.1: pyridine / N,N-dimethyl-formamide; 1,4-dioxane / 0.17 h / 20 °C

9
[ 35542-01-9 ]
2′,3′-O-methoxymethylidene-5-OMe-uridine [ No CAS ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 3 steps 1.1: toluene-4-sulfonic acid / 24 h / 20 °C 1.2: Dowex / 3 h / 20 °C 2.1: pyridine / N,N-dimethyl-formamide; 1,4-dioxane / 0.17 h / 20 °C 3.1: tributyl-amine / N,N-dimethyl-formamide; 1,4-dioxane / 0.08 h / 20 °C

10
[ 35542-01-9 ]
2′,3′-O-methoxymethylidene-5-OMe-uridine [ No CAS ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 4 steps 1.1: toluene-4-sulfonic acid / 24 h / 20 °C 1.2: Dowex / 3 h / 20 °C 2.1: pyridine / N,N-dimethyl-formamide; 1,4-dioxane / 0.17 h / 20 °C 3.1: tributyl-amine / N,N-dimethyl-formamide; 1,4-dioxane / 0.08 h / 20 °C 4.1: dimethylsulfide borane complex / N,N-dimethyl-formamide; 1,4-dioxane; tetrahydrofuran / 0.25 h / 20 °C

11
[ 35542-01-9 ]
C₁₀H₁₆BN₂O₁₂P₂^(3-)*3Na⁽¹⁺⁾ [ No CAS ]
5-OMe-uridine-5'-O-(α-boranotriphosphate) [ No CAS ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 5 steps 1.1: toluene-4-sulfonic acid / 24 h / 20 °C 1.2: Dowex / 3 h / 20 °C 2.1: pyridine / N,N-dimethyl-formamide; 1,4-dioxane / 0.17 h / 20 °C 3.1: tributyl-amine / N,N-dimethyl-formamide; 1,4-dioxane / 0.08 h / 20 °C 4.1: dimethylsulfide borane complex / N,N-dimethyl-formamide; 1,4-dioxane; tetrahydrofuran / 0.25 h / 20 °C 5.1: ethylenediamine / N,N-dimethyl-formamide; 1,4-dioxane; tetrahydrofuran / 0.17 h / 20 °C 5.2: 3 h / 20 °C / pH 2.3

12
[ 35542-01-9 ]
C₁₀H₁₆BN₂O₉P^(2-)*2Na⁽¹⁺⁾ [ No CAS ]
5-Ome-uridine-H-phosphate [ No CAS ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 6 steps 1.1: toluene-4-sulfonic acid / 24 h / 20 °C 1.2: Dowex / 3 h / 20 °C 2.1: pyridine / N,N-dimethyl-formamide; 1,4-dioxane / 0.17 h / 20 °C 3.1: tributyl-amine / N,N-dimethyl-formamide; 1,4-dioxane / 0.08 h / 20 °C 4.1: dimethylsulfide borane complex / N,N-dimethyl-formamide; 1,4-dioxane; tetrahydrofuran / 0.25 h / 20 °C 5.1: ethylenediamine / N,N-dimethyl-formamide; 1,4-dioxane; tetrahydrofuran / 0.17 h / 20 °C 5.2: 3 h / 20 °C / pH 2.3 6.1: hydrogenchloride; potassium chloride / water-d2 / 37 °C / pH 1.4

13
[ 35542-01-9 ]
5-methoxy-UTP [ No CAS ]

Yield	Reaction Conditions	Operation in experiment
	Stage #1: 5-methoxycarbonylmethyl-2'-O-methyl-uridine With trimethyl phosphite; trichlorophosphate at 0℃; for 2h; Inert atmosphere; Stage #2: With tributyl-amine; bis(tri-n-butylammonium) pyrophosphate In acetonitrile for 0.416667h; Stage #3: With tetraethylammonium bromide In acetonitrile at 20℃; for 1h;	32 Synthesis of 5-methoxy uridine (compound 15) and 5-methoxy UTP (NTP of said compound) A solution of 5-methoxy uridine (compound 1 5) (69.0 mg, 0.25 mmol, plus heat to make it soluble) was added to proton sponge (80.36 mg, 0.375 mmol, 1 .50 equiv.) in 0.7 ml_ trimethylphosphate (TMP) and was stirred for 10 minutes at 0SC. Phosphorous oxychloride (POCI3) (46.7 ul, 0.50 mmol, 2.0 equiv.) was added dropwise to the solution before being kept stirring for 2 hours under N2 atmosphere. After 2 hours the solution was reacted with a mixture of bistributylammonium pyrophosphate (TBAPP or (894.60 mg, 1 .63 mmol, 6.50 equiv.) and tributylamine (243.0 ul, 1 .00 mmol, 4.0 equiv.) in 2.0 ml of dimethylformamide. After approximately 15 minutes, the reaction was quenched with 17.0 ml of 0.2M triethylammonium bicarbonate (TEAB) and the clear solution was stirred at room temperature for an hour. The reaction mixture was lyophilized overnight and the crude reaction mixture was purified by HPLC (Shimadzu, Kyoto Japan, Phenomenex C18 preparative column, 250 x 21 .20 mm, 10.0 micron; gradient: 1 00 % A for 3.0 min, then 1 % B/min, A = 100 mM TEAB buffer, B = ACN ; flow rate: 10.0 m L/min; retention time: 16.57-17.51 min). Fractions containing the desired compound were pooled and lyophilized to produce the NTP of compound 15. The triphosphorylation reactions were carried out in a two-neck flask flame-dried under N2 atmosphere. Nucleosides and the protein sponge were dried over P205 under vacuum overnight prior to use. The formation of monophosphates was monitored by LCMS.

Reference： [1]Current Patent Assignee: MODERNA THERAPEUTICS INC - WO2014/93924, 2014, A1 Location in patent: Page/Page column 265

14
[ 15715-58-9 ]
bis(tri-n-butylammonium) pyrophosphate [ No CAS ]
[ 35542-01-9 ]

Yield	Reaction Conditions	Operation in experiment
	With tributyl-amine; trichlorophosphate	32 Example 32. Example 32. Synthesis of 5-methoxy uridine (compound 15) and 5-methoxy UTP (NTP of said compound) A solution of 5-methoxy uridine (compound 15) (69.0 mg, 0.25 mmol, plus heat to make it soluble) was added to proton sponge (80.36 mg, 0.375 mmol, 1.50 equiv.) in 0.7 mL trimethylphosphate (TMP) and was stirred for 10 minutes at 0°C. Phosphorous oxychloride (POCl3) (46.7 ul, 0.50 mmol, 2.0 equiv.) was added dropwise to the solution before being kept stirring for 2 hours under N2 atmosphere. After 2 hours the solution was reacted with a mixture of bistributylammonium pyrophosphate (TBAPP or (n-Bu3NH)2H2P2O7) (894.60 mg, 1.63 mmol, 6.50 equiv.) and tributylamine (243.0 ul, 1.00 mmol, 4.0 equiv.) in 2.0 ml of dimethylformamide. After approximately 15 minutes, the reaction was quenched with 17.0 ml of 0.2M triethylammonium bicarbonate (TEAB) and the clear solution was stirred at room temperature for an hour. The reaction mixture was lyophilized overnight and the crude reaction mixture was purified by HPLC (Shimadzu, Kyoto Japan, Phenomenex C18 preparative column, 250 x 21.20 mm, 10.0 micron; gradient: 100 % A for 3.0 min, then 1% B/min, A = 100 mM TEAB buffer, B = ACN; flow rate: 10.0 mL/min; retention time: 16.57-17.51 min). Fractions containing the desired compound were pooled and lyophilized to produce the NTP of compound 15. The triphosphorylation reactions were carried out in a two-neck flask flame-dried under N2 atmosphere. Nucleosides and the protein sponge were dried over P2O5 under vacuum overnight prior to use. The formation of monophosphates was monitored by LCMS.

Reference： [1]Current Patent Assignee: MODERNA THERAPEUTICS INC - EP2918275, 2015, A1

15
[ 30771-43-8 ]
[ 35542-01-9 ]
5-methoxy-4-pyrimidinone 1-β-D-ribofuranoside [ No CAS ]

Yield	Reaction Conditions	Operation in experiment
	With dihydrogen peroxide In aq. phosphate buffer at 25℃;
	With dihydrogen peroxide In aq. phosphate buffer at 25℃;

Reference： [1]Bartos, Paulina; Ebenryter-Olbinska, Katarzyna; Sochacka, Elzbieta; Nawrot, Barbara [Bioorganic and Medicinal Chemistry, 2015, vol. 23, # 17, p. 5587 - 5594]
[2]Bartos, Paulina; Ebenryter-Olbinska, Katarzyna; Sochacka, Elzbieta; Nawrot, Barbara [Bioorganic and Medicinal Chemistry, 2015, vol. 23, # 17, p. 5587 - 5594]

16
[ 37805-86-0 ]
[ 35542-01-9 ]

Yield	Reaction Conditions	Operation in experiment
	With sodium methylate

Reference： [1]Meltzer, Diana; Ethan, Ophir; Arguin, Guillaume; Nadel, Yael; Danino, Ortal; Lecka, Joanna; Sévigny, Jean; Gendron, Fernand-Pierre; Fischer, Bilha [Bioorganic and Medicinal Chemistry, 2015, vol. 23, # 17, p. 5764 - 5773]

17
[ 35542-01-9 ]
2C₆H₁₅N*C₁₁H₁₇N₂O₁₂PS [ No CAS ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 4 steps 1.1: toluene-4-sulfonic acid / acetone 2.1: pyridine / 1,4-dioxane 2.2: 0.5 h / 60 °C / Microwave irradiation 3.1: sodium sulfite; sodium hydroxide / water / 130 °C / pH 9 - 10 / Microwave irradiation 4.1: acetic acid; water / 2 h / 95 °C

18
[ 35542-01-9 ]
C₂₀H₂₄N₂O₉S [ No CAS ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 2 steps 1: toluene-4-sulfonic acid / acetone 2: pyridine / 5 h / 4 °C

19
[ 35542-01-9 ]
2C₆H₁₅N*C₁₄H₂₁N₂O₁₂PS [ No CAS ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 3 steps 1.1: toluene-4-sulfonic acid / acetone 2.1: pyridine / 1,4-dioxane 2.2: 0.5 h / 60 °C / Microwave irradiation 3.1: sodium sulfite; sodium hydroxide / water / 130 °C / pH 9 - 10 / Microwave irradiation

20
[ 35542-01-9 ]
3Na⁽¹⁺⁾*C₁₁H₁₅N₂O₁₂P₂^(3-) [ No CAS ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 3 steps 1.1: toluene-4-sulfonic acid / acetone 2.1: pyridine / 5 h / 4 °C 3.1: N,N-dimethyl-formamide / 72 h / 70 °C 3.2: 95 °C 3.3: CM Sephadex sodium-form ion-exchange resin column

21
[ 35542-01-9 ]
3Na⁽¹⁺⁾*C₁₁H₁₃Cl₂N₂O₁₂P₂^(3-) [ No CAS ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 3 steps 1.1: toluene-4-sulfonic acid / acetone 2.1: pyridine / 5 h / 4 °C 3.1: N,N-dimethyl-formamide / 72 h / 70 °C 3.2: 95 °C 3.3: CM Sephadex sodium-form ion-exchange resin column

22
[ 35542-01-9 ]
3Na⁽¹⁺⁾*C₁₁H₁₃F₂N₂O₁₂P₂^(3-) [ No CAS ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 3 steps 1.1: toluene-4-sulfonic acid / acetone 2.1: pyridine / 5 h / 4 °C 3.1: N,N-dimethyl-formamide / 72 h / 70 °C 3.2: 95 °C 3.3: CM Sephadex sodium-form ion-exchange resin column

23
[ 77-76-9 ]
[ 35542-01-9 ]
C₁₃H₁₈N₂O₇ [ No CAS ]

Yield	Reaction Conditions	Operation in experiment
	With toluene-4-sulfonic acid In acetone

24
[ 35542-01-9 ]
[ 847649-65-4 ]

Yield	Reaction Conditions	Operation in experiment
	Stage #1: 5-methoxycarbonylmethyl-2'-O-methyl-uridine With trimethyl phosphite; N,N,N',N'-tetramethyl-1,8-diaminonaphthalene at 0℃; for 0.166667h; Stage #2: With trichlorophosphate for 2h; Inert atmosphere; Stage #3: With tributyl-amine; bis(tributylammonium) pyrophosphate In N,N-dimethyl-formamide for 0.25h;	32 Example 32. Synthesis of 5-methoxy uridine (compound 15) and 5-methoxy UTP (NTP of said compound) A solution of 5-methoxy uridine (compound 15) (69.0 mg, 0.25 mmol, plus heat to make itsoluble) was added to proton sponge (80.36 mg, 0.375 mmol, 1.50 equiv.) in 0.7 mLtrimethylphosphate (TMP) and was stirred for 10 minutes at 0C. Phosphorous oxychloride (POOl3)(46.7 ul, 0.50 mmol, 2.0 equiv.) was added dropwise to the solution before being kept stirring for 2hours under N2 atmosphere. After 2 hours the solution was reacted with a mixture ofbistributylammonium pyrophosphate (TBAPP or (n-Bu3NH)2H2P207) (894.60 mg, 1.63 mmol, 6.50 equiv.) and tributylamine (243.0 ul, 1.00 mmol, 4.0 equiv.) in 2.0 ml of dimethylformamide. After approximately 15 minutes, the reaction was quenched with 17.0 ml of 0.2M triethylammonium bicarbonate (TEAB) and the clear solution was stirred at room temperature for an hour. The reactionmixture was lyophilized overnight and the crude reaction mixture was purified by HPLC (Shimadzu, Kyoto Japan, Phenomenex 018 preparative column, 250 x 21 .20 mm, 10.0 micron; gradient: 100 % A for 3.0 mm, then 1% B/mm, A = 100 mM TEAB buffer, B = ACN; flow rate: 10.0 mL/min; retention time: 16.57-1 7.51 mm). Fractions containing the desired compound were pooled and lyophilized to produce the NTP of compound 15. The triphosphorylation reactions were carried out in a two-neckflask flame-dried under N2 atmosphere. Nucleosides and the protein sponge were dried over P205 under vacuum overnight prior to use. The formation of monophosphates was monitored by LOMS.
	Stage #1: 5-methoxycarbonylmethyl-2'-O-methyl-uridine With N,N,N',N'-tetramethyl-1,8-diaminonaphthalene at 0℃; for 0.166667h; Stage #2: With trichlorophosphate for 2h; Inert atmosphere; Further stages;	25; 32 Example 32. Synthesis of 5-methoxy uridine (compound 15) and 5-methoxy UTP (NTP of said mpound) Example 32. Synthesis of 5-methoxy uridine (compound 15) and 5-methoxy UTP (NTP of said mpound) A solution of 5-methoxy uridine (compound 1 5) (69.0 mg, 0.25 mmol, plus heat to make it uble) was added to proton sponge (80.36 mg, 0.375 mmol, 1 .50 equiv.) in 0.7 ml_ methylphosphate (TM P) and was stirred for 1 0 minutes at 0SC. Phosphorous oxychloride (POCI3) .7 ul, 0.50 mmol, 2.0 equiv.) was added dropwise to the solution before being kept stirring for 2 urs under N2 atmosphere. After 2 hours the solution was reacted with a mixture of tributylammonium pyrophosphate (TBAPP or (n-Bu3N H)2H2P207) (894.60 mg, 1 .63 mmol, 6.50 uiv.) and tributylamine (243.0 ul, 1 .00 mmol, 4.0 equiv.) in 2.0 ml of dimethylformamide. After proximately 1 5 minutes, the reaction was quenched with 17.0 ml of 0.2M triethylammonium arbonate (TEAB) and the clear solution was stirred at room temperature for an hour. The reaction xture was lyophilized overnight and the crude reaction mixture was purified by H PLC (Shimadzu, oto Japan, Phenomenex C18 preparative column, 250 x 21 .20 mm, 10.0 micron; gradient: 1 00 % A 3.0 min, then 1 % B/min, A = 100 mM TEAB buffer, B = ACN; flow rate: 1 0.0 mL/min; retention e: 16.57-1 7.51 min). Fractions containing the desired compound were pooled and lyophilized to duce the NTP of compound 15. The triphosphorylation reactions were carried out in a two-neck sk flame-dried under N2 atmosphere. Nucleosides and the protein sponge were dried over P205 der vacuum overnight prior to use. The formation of monophosphates was monitored by LCMS.

Reference： [1]Current Patent Assignee: MODERNA THERAPEUTICS INC - WO2015/196130, 2015, A2 Location in patent: Page/Page column 598
[2]Current Patent Assignee: MODERNA THERAPEUTICS INC - WO2015/196128, 2015, A2 Location in patent: Page/Page column 606

25
[ 28708-32-9 ]
[ 35542-01-9 ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 2 steps 1.1: N,O-bis(trimethylsilane)acetamide / acetonitrile / 20 °C 1.2: 0 - 20 °C 2.1: ammonia / methanol / 20 °C

Reference： [1]Current Patent Assignee: TOSK - US9382287, 2016, B2

26
2′,3′,5′-tri-O-acetyl-5-methoxyuridine [ No CAS ]
[ 35542-01-9 ]

Yield	Reaction Conditions	Operation in experiment
	With ammonia In methanol at 20℃;	B. Representative Procedure for Acetate Hydrolysis Reaction Ribonucleoside tetraacetate 3A-D was dissolved in a 7N solution of ammonia in methanol. The solution was stirred overnight at room temperature. The solution was then reduced in vacuo. The resultant residue was triturated with ether to give the free ribonucleoside as a white solid that was isolated by filtration and dried under high vacuum before the next step.

Reference： [1]Current Patent Assignee: TOSK - US9382287, 2016, B2 Location in patent: Page/Page column 44; 45

27
[ 35542-01-9 ]
4C₆H₁₅N*C₁₀H₁₇N₂O₁₆P₃ [ No CAS ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 2 steps 1.1: trimethyl phosphite; N,N,N',N'-tetramethyl-1,8-diaminonaphthalene / 0.17 h / 0 °C 1.2: 2 h / Inert atmosphere 2.1: tributyl-amine; bis(tri-n-butylammonium) pyrophosphate / N,N-dimethyl-formamide / 0.25 h 2.2: 3407 / 1 h / 20 °C

Reference： [1]Current Patent Assignee: MODERNA THERAPEUTICS INC - EP2918275, 2015, A1

28
N-cyclohexyl-N'-β-(4-methylmorpholinium)ethylcarbodiimide p-toluenesulfonate-(methyl-D₃) [ No CAS ]
[ 35542-01-9 ]
mo5U-D3-CMCT [ No CAS ]

Yield	Reaction Conditions	Operation in experiment
	In aq. buffer at 40℃; for 14h;

Reference： [1]Cheng, Qing-Yun; Xiong, Jun; Ma, Cheng-Jie; Dai, Yi; Ding, Jiang-Hui; Liu, Fei-Long; Yuan, Bi-Feng; Feng, Yu-Qi [Chemical Science, 2020, vol. 11, # 7, p. 1878 - 1891]

29
[ 2491-17-0 ]
[ 35542-01-9 ]
mo5U-CMCT [ No CAS ]

Yield	Reaction Conditions	Operation in experiment
	In aq. buffer at 40℃; for 14h;

Reference： [1]Cheng, Qing-Yun; Xiong, Jun; Ma, Cheng-Jie; Dai, Yi; Ding, Jiang-Hui; Liu, Fei-Long; Yuan, Bi-Feng; Feng, Yu-Qi [Chemical Science, 2020, vol. 11, # 7, p. 1878 - 1891]

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Precautionary Statements-General
Code	Phrase
P101	If medical advice is needed,have product container or label at hand.
P102	Keep out of reach of children.
P103	Read label before use
Prevention
Code	Phrase
P201	Obtain special instructions before use.
P202	Do not handle until all safety precautions have been read and understood.
P210	Keep away from heat/sparks/open flames/hot surfaces. - No smoking.
P211	Do not spray on an open flame or other ignition source.
P220	Keep/Store away from clothing/combustible materials.
P221	Take any precaution to avoid mixing with combustibles
P222	Do not allow contact with air.
P223	Keep away from any possible contact with water, because of violent reaction and possible flash fire.
P230	Keep wetted
P231	Handle under inert gas.
P232	Protect from moisture.
P233	Keep container tightly closed.
P234	Keep only in original container.
P235	Keep cool
P240	Ground/bond container and receiving equipment.
P241	Use explosion-proof electrical/ventilating/lighting/equipment.
P242	Use only non-sparking tools.
P243	Take precautionary measures against static discharge.
P244	Keep reduction valves free from grease and oil.
P250	Do not subject to grinding/shock/friction.
P251	Pressurized container: Do not pierce or burn, even after use.
P260	Do not breathe dust/fume/gas/mist/vapours/spray.
P261	Avoid breathing dust/fume/gas/mist/vapours/spray.
P262	Do not get in eyes, on skin, or on clothing.
P263	Avoid contact during pregnancy/while nursing.
P264	Wash hands thoroughly after handling.
P265	Wash skin thouroughly after handling.
P270	Do not eat, drink or smoke when using this product.
P271	Use only outdoors or in a well-ventilated area.
P272	Contaminated work clothing should not be allowed out of the workplace.
P273	Avoid release to the environment.
P280	Wear protective gloves/protective clothing/eye protection/face protection.
P281	Use personal protective equipment as required.
P282	Wear cold insulating gloves/face shield/eye protection.
P283	Wear fire/flame resistant/retardant clothing.
P284	Wear respiratory protection.
P285	In case of inadequate ventilation wear respiratory protection.
P231 + P232	Handle under inert gas. Protect from moisture.
P235 + P410	Keep cool. Protect from sunlight.
Response
Code	Phrase
P301	IF SWALLOWED:
P304	IF INHALED:
P305	IF IN EYES:
P306	IF ON CLOTHING:
P307	IF exposed:
P308	IF exposed or concerned:
P309	IF exposed or if you feel unwell:
P310	Immediately call a POISON CENTER or doctor/physician.
P311	Call a POISON CENTER or doctor/physician.
P312	Call a POISON CENTER or doctor/physician if you feel unwell.
P313	Get medical advice/attention.
P314	Get medical advice/attention if you feel unwell.
P315	Get immediate medical advice/attention.
P320
P302 + P352	IF ON SKIN: wash with plenty of soap and water.
P321
P322
P330	Rinse mouth.
P331	Do NOT induce vomiting.
P332	IF SKIN irritation occurs:
P333	If skin irritation or rash occurs:
P334	Immerse in cool water/wrap n wet bandages.
P335	Brush off loose particles from skin.
P336	Thaw frosted parts with lukewarm water. Do not rub affected area.
P337	If eye irritation persists:
P338	Remove contact lenses, if present and easy to do. Continue rinsing.
P340	Remove victim to fresh air and keep at rest in a position comfortable for breathing.
P341	If breathing is difficult, remove victim to fresh air and keep at rest in a position comfortable for breathing.
P342	If experiencing respiratory symptoms:
P350	Gently wash with plenty of soap and water.
P351	Rinse cautiously with water for several minutes.
P352	Wash with plenty of soap and water.
P353	Rinse skin with water/shower.
P360	Rinse immediately contaminated clothing and skin with plenty of water before removing clothes.
P361	Remove/Take off immediately all contaminated clothing.
P362	Take off contaminated clothing and wash before reuse.
P363	Wash contaminated clothing before reuse.
P370	In case of fire:
P371	In case of major fire and large quantities:
P372	Explosion risk in case of fire.
P373	DO NOT fight fire when fire reaches explosives.
P374	Fight fire with normal precautions from a reasonable distance.
P376	Stop leak if safe to do so. Oxidising gases (section 2.4) 1
P377	Leaking gas fire: Do not extinguish, unless leak can be stopped safely.
P378
P380	Evacuate area.
P381	Eliminate all ignition sources if safe to do so.
P390	Absorb spillage to prevent material damage.
P391	Collect spillage. Hazardous to the aquatic environment
P301 + P310	IF SWALLOWED: Immediately call a POISON CENTER or doctor/physician.
P301 + P312	IF SWALLOWED: call a POISON CENTER or doctor/physician IF you feel unwell.
P301 + P330 + P331	IF SWALLOWED: Rinse mouth. Do NOT induce vomiting.
P302 + P334	IF ON SKIN: Immerse in cool water/wrap in wet bandages.
P302 + P350	IF ON SKIN: Gently wash with plenty of soap and water.
P303 + P361 + P353	IF ON SKIN (or hair): Remove/Take off Immediately all contaminated clothing. Rinse SKIN with water/shower.
P304 + P312	IF INHALED: Call a POISON CENTER or doctor/physician if you feel unwell.
P304 + P340	IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing.
P304 + P341	IF INHALED: If breathing is difficult, remove victim to fresh air and keep at rest in a position comfortable for breathing.
P305 + P351 + P338	IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P306 + P360	IF ON CLOTHING: Rinse Immediately contaminated CLOTHING and SKIN with plenty of water before removing clothes.
P307 + P311	IF exposed: call a POISON CENTER or doctor/physician.
P308 + P313	IF exposed or concerned: Get medical advice/attention.
P309 + P311	IF exposed or if you feel unwell: call a POISON CENTER or doctor/physician.
P332 + P313	IF SKIN irritation occurs: Get medical advice/attention.
P333 + P313	IF SKIN irritation or rash occurs: Get medical advice/attention.
P335 + P334	Brush off loose particles from skin. Immerse in cool water/wrap in wet bandages.
P337 + P313	IF eye irritation persists: Get medical advice/attention.
P342 + P311	IF experiencing respiratory symptoms: call a POISON CENTER or doctor/physician.
P370 + P376	In case of fire: Stop leak if safe to Do so.
P370 + P378	In case of fire:
P370 + P380	In case of fire: Evacuate area.
P370 + P380 + P375	In case of fire: Evacuate area. Fight fire remotely due to the risk of explosion.
P371 + P380 + P375	In case of major fire and large quantities: Evacuate area. Fight fire remotely due to the risk of explosion.
Storage
Code	Phrase
P401
P402	Store in a dry place.
P403	Store in a well-ventilated place.
P404	Store in a closed container.
P405	Store locked up.
P406	Store in corrosive resistant/ container with a resistant inner liner.
P407	Maintain air gap between stacks/pallets.
P410	Protect from sunlight.
P411
P412	Do not expose to temperatures exceeding 50 oC/ 122 oF.
P413
P420	Store away from other materials.
P422
P402 + P404	Store in a dry place. Store in a closed container.
P403 + P233	Store in a well-ventilated place. Keep container tightly closed.
P403 + P235	Store in a well-ventilated place. Keep cool.
P410 + P403	Protect from sunlight. Store in a well-ventilated place.
P410 + P412	Protect from sunlight. Do not expose to temperatures exceeding 50 oC/122oF.
P411 + P235	Keep cool.
Disposal
Code	Phrase
P501	Dispose of contents/container to ...
P502	Refer to manufacturer/supplier for information on recovery/recycling

Purity	Size	Price	VIP Price	USA Stock *0-1 Day	Global Stock *5-7 Days	Quantity
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Physical hazards
Code	Phrase
H200	Unstable explosive
H201	Explosive; mass explosion hazard
H202	Explosive; severe projection hazard
H203	Explosive; fire, blast or projection hazard
H204	Fire or projection hazard
H205	May mass explode in fire
H220	Extremely flammable gas
H221	Flammable gas
H222	Extremely flammable aerosol
H223	Flammable aerosol
H224	Extremely flammable liquid and vapour
H225	Highly flammable liquid and vapour
H226	Flammable liquid and vapour
H227	Combustible liquid
H228	Flammable solid
H229	Pressurized container: may burst if heated
H230	May react explosively even in the absence of air
H231	May react explosively even in the absence of air at elevated pressure and/or temperature
H240	Heating may cause an explosion
H241	Heating may cause a fire or explosion
H242	Heating may cause a fire
H250	Catches fire spontaneously if exposed to air
H251	Self-heating; may catch fire
H252	Self-heating in large quantities; may catch fire
H260	In contact with water releases flammable gases which may ignite spontaneously
H261	In contact with water releases flammable gas
H270	May cause or intensify fire; oxidizer
H271	May cause fire or explosion; strong oxidizer
H272	May intensify fire; oxidizer
H280	Contains gas under pressure; may explode if heated
H281	Contains refrigerated gas; may cause cryogenic burns or injury
H290	May be corrosive to metals
Health hazards
Code	Phrase
H300	Fatal if swallowed
H301	Toxic if swallowed
H302	Harmful if swallowed
H303	May be harmful if swallowed
H304	May be fatal if swallowed and enters airways
H305	May be harmful if swallowed and enters airways
H310	Fatal in contact with skin
H311	Toxic in contact with skin
H312	Harmful in contact with skin
H313	May be harmful in contact with skin
H314	Causes severe skin burns and eye damage
H315	Causes skin irritation
H316	Causes mild skin irritation
H317	May cause an allergic skin reaction
H318	Causes serious eye damage
H319	Causes serious eye irritation
H320	Causes eye irritation
H330	Fatal if inhaled
H331	Toxic if inhaled
H332	Harmful if inhaled
H333	May be harmful if inhaled
H334	May cause allergy or asthma symptoms or breathing difficulties if inhaled
H335	May cause respiratory irritation
H336	May cause drowsiness or dizziness
H340	May cause genetic defects
H341	Suspected of causing genetic defects
H350	May cause cancer
H351	Suspected of causing cancer
H360	May damage fertility or the unborn child
H361	Suspected of damaging fertility or the unborn child
H361d	Suspected of damaging the unborn child
H362	May cause harm to breast-fed children
H370	Causes damage to organs
H371	May cause damage to organs
H372	Causes damage to organs through prolonged or repeated exposure
H373	May cause damage to organs through prolonged or repeated exposure
Environmental hazards
Code	Phrase
H400	Very toxic to aquatic life
H401	Toxic to aquatic life
H402	Harmful to aquatic life
H410	Very toxic to aquatic life with long-lasting effects
H411	Toxic to aquatic life with long-lasting effects
H412	Harmful to aquatic life with long-lasting effects
H413	May cause long-lasting harmful effects to aquatic life
H420	Harms public health and the environment by destroying ozone in the upper atmosphere

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[ 35542-01-9 ] Synthesis Path-Downstream 1~29

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