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3.3. Procedure
One milliliter of the KT solution (containing 100-1000 g/mL) or (25-400 g/mL)was transferred into 10 mL flasks, then 1 mL of the acceptors (DDQ and TCNQ) (1 mg/mL)was added, and the volume was made up with acetonitrile. The resultant red color wasmeasured by spectrophotometry at 485 nm. In the case of TCNQ, the color was measuredat 843 nm after heating at 70 C for 20 min, against a reagent blank prepared by the sameway without KT. Using serial dilutions, calibration curves were constructed (absorbance vs.concentration of KT) for both complexes.
3.3. Procedure
One milliliter of the KT solution (containing 100-1000 g/mL) or (25-400 g/mL)was transferred into 10 mL flasks, then 1 mL of the acceptors (DDQ and TCNQ) (1 mg/mL)was added, and the volume was made up with acetonitrile. The resultant red color wasmeasured by spectrophotometry at 485 nm. In the case of TCNQ, the color was measuredat 843 nm after heating at 70 C for 20 min, against a reagent blank prepared by the sameway without KT. Using serial dilutions, calibration curves were constructed (absorbance vs.concentration of KT) for both complexes.