Alternatived Products of [ 18194-25-7 ]
Product Details of [ 18194-25-7 ]
CAS No. : | 18194-25-7 |
MDL No. : | MFCD00066561 |
Formula : |
C32H64NO8P
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Boiling Point : |
- |
Linear Structure Formula : | - |
InChI Key : | IJFVSSZAOYLHEE-SSEXGKCCSA-N |
M.W : |
621.83
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Pubchem ID : | 512874 |
Synonyms : |
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Application In Synthesis of [ 18194-25-7 ]
* All experimental methods are cited from the reference, please refer to the original source for details. We do not guarantee the accuracy of the content in the reference.
- Downstream synthetic route of [ 18194-25-7 ]
Yield | Reaction Conditions | Operation in experiment |
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With P-1 phospholipase from Bothrops neuwiedii; sodium chloride; calcium chloride In hydrogenchloride at 20℃; Enzymatic reaction; |
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With phospholipase A2 from porcine pancreas; water; sodium cholate; sodium chloride; calcium chloride at 37℃; aq. buffer; Enzymatic reaction; |
2.7. Determination of the kinetic constants of ppPLA2 by pH-stat titration
General procedure: For the preparation of the substrate stock solution, 50 μmol of 1,2- or 1,3-PCs with acyl chain lengths of C10-C16 were dissolved in 500 μL of chloroform. The solvent was evaporated under vacuum, and the lipid film was dissolved in 10 mL of 5 mM sodium deoxycholate containing 100 mM NaCl by vortexing for 15 min as described above. The assay solution (1.5 mL) contained 20-1000 μL of the substrate stock solution, 500 μL of 1 mM Tris/HCl buffer, pH 8.0 with 100 mM NaCl and 16 mM CaCl2, and 980-0 μL H2O. The pH of the reaction mixture was adjusted to 8.0, and the reaction was started at 37 °C by addition of 5 μL (62.5 mg mL-1) of enzyme solution. The amount of liberated fatty acid was determined by continuous titration (3-6 min) with 2 mM NaOH. For the determination of the kinetic constants, initial reaction rates were determined at substrate concentrations between 0.17 and 5 mM and expressed as μmol of liberated fatty acid per minute and mg protein. KM,app and Vmax,app were obtained by non-linear regression using the Michaelis-Menten function in Sigma-Plot 8.0. All data are the means of two independent experimental series. |
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With Fusarium solani lipase In aq. buffer at 25℃; Enzymatic reaction; |
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- 2
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[ 18194-25-7 ]
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[ 50-89-5 ]
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C33H57N2PO12(CH2)4
[ No CAS ]
Yield | Reaction Conditions | Operation in experiment |
50% |
With D-glucose In chloroform; acetate buffer at 37℃; for 1.5h; |
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Yield | Reaction Conditions | Operation in experiment |
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The method of claim 4 wherein the phospholipid is selected from the group consisting of 1,2-dioleoyl-sn-glycero 3-phosphocholine, 1,2-dilauroyl-sn-glycero-3-phosphocholine, 1,2-dimyristoy-sn-glycero-3-phosphocholine, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine, 1,2-distearoyl-sn-glycero-3-phosphocholine, 1,2-diarachidoyl-sn-glycero-phosphocholine, 1,2-dibehenoyl-sn-glycero-3-phospbocholine, 1,2-dipalmitoleoyl-sn-glycero-3-phosphocholine, 1,2-dieicosenoyl-sn-glycero-3-phosphocholine, 1,2-dierucoyl-sn-glycero-3-phosphocholine, ... |
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Other representative phospholipids of Formula I include: ... 1,3-dipalmitoyl-sn-glycero-2-phosphocholine; 1,2-distearoyl-sn-glycero-3-phospho-(N-methyl)ethanolamine; 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine; 1,3-distearoyl-sn-glycero-2-phospho-(N,N-dimethyl) ethanolamine; 1,2 - dilauroyl- sn-glycero -3 -phosphocholine; 1,2-dieicosanoyl-sn-glycero-3-phosphocholine; 1-oleoyl-3-myristoyl-sn-glycero-3-phospho-(N-methyl) ethanolamine; 1,2-di-(9-chloro-octadecanoyl)-sn-glycero-3-phosphocholine; ... |
- 4
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[ 18194-25-7 ]
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C32H64NO8P*4C56H98O35
[ No CAS ]
Yield | Reaction Conditions | Operation in experiment |
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In chloroform; water at 40℃; for 0.0833333h; |
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Reference:
[1]Ikeda, Atsushi; Iwata, Noboru; Hino, Shodai; Mae, Tomoya; Tsuchiya, Yuki; Sugikawa, Kouta; Hirao, Takehiro; Haino, Takeharu; Ohara, Kazuaki; Yamaguchi, Kentaro
[RSC Advances, 2015, vol. 5, # 95, p. 77746 - 77754]
- 5
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[ 51166-71-3 ]
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[ 18194-25-7 ]
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3C56H98O35*C32H64NO8P
[ No CAS ]
Yield | Reaction Conditions | Operation in experiment |
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In water-d2 at 20℃; for 6h; |
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