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CAS No. : | 1229705-06-9 | MDL No. : | MFCD26142931 |
Formula : | C31H29Cl2F2N3O4 | Boiling Point : | - |
Linear Structure Formula : | - | InChI Key : | TVTXCJFHQKSQQM-LJQIRTBHSA-N |
M.W : | 616.48 | Pubchem ID : | 53358942 |
Synonyms : |
RG7388;Ro 5503781
|
Num. heavy atoms : | 42 |
Num. arom. heavy atoms : | 18 |
Fraction Csp3 : | 0.32 |
Num. rotatable bonds : | 9 |
Num. H-bond acceptors : | 8.0 |
Num. H-bond donors : | 3.0 |
Molar Refractivity : | 160.35 |
TPSA : | 111.45 Ų |
GI absorption : | Low |
BBB permeant : | No |
P-gp substrate : | Yes |
CYP1A2 inhibitor : | No |
CYP2C19 inhibitor : | No |
CYP2C9 inhibitor : | No |
CYP2D6 inhibitor : | Yes |
CYP3A4 inhibitor : | Yes |
Log Kp (skin permeation) : | -7.01 cm/s |
Log Po/w (iLOGP) : | 3.96 |
Log Po/w (XLOGP3) : | 4.29 |
Log Po/w (WLOGP) : | 7.21 |
Log Po/w (MLOGP) : | 4.75 |
Log Po/w (SILICOS-IT) : | 6.96 |
Consensus Log Po/w : | 5.43 |
Lipinski : | 2.0 |
Ghose : | None |
Veber : | 0.0 |
Egan : | 1.0 |
Muegge : | 1.0 |
Bioavailability Score : | 0.17 |
Log S (ESOL) : | -6.09 |
Solubility : | 0.000503 mg/ml ; 0.000000817 mol/l |
Class : | Poorly soluble |
Log S (Ali) : | -6.34 |
Solubility : | 0.00028 mg/ml ; 0.000000454 mol/l |
Class : | Poorly soluble |
Log S (SILICOS-IT) : | -10.56 |
Solubility : | 0.0000000171 mg/ml ; 0.0 mol/l |
Class : | Insoluble |
PAINS : | 0.0 alert |
Brenk : | 0.0 alert |
Leadlikeness : | 3.0 |
Synthetic accessibility : | 4.98 |
Signal Word: | Warning | Class: | N/A |
Precautionary Statements: | P261-P305+P351+P338 | UN#: | N/A |
Hazard Statements: | H302-H315-H319-H335 | Packing Group: | N/A |
GHS Pictogram: |
* All experimental methods are cited from the reference, please refer to the original source for details. We do not guarantee the accuracy of the content in the reference.
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
40.8% | In dichloromethane; at 12℃; for 0.25h;microwave irradiation; | To a lo mL microwave vial was added chiral 4-[(2R,3S,4R,5S)-3-(3-chloro-2-fluoro-phenyl)-4-(4-chloro-2-fluoro-phenyl)-4-cyano-5-(2,2-dimethyl-propyl)-pyrrolidine-2-carbonyl]-amino}-3-methoxy-benzoic acid (31.0 mg, 0.050 mmol) and ethyl 2-isocyanatoacetate (6.49 mg, 0.050 mmol) in CH2Cl2 (3 ml) to give a suspension. The vial was capped and heated in the microwave at 120 C. for 15 min. The reaction mixture was poured into water (5 mL) and extracted with EtOA (3*20 mL). The organic layer was separated and concentrated under reduced pressure to afford crude product which was purified by RP-HPLC to give chiral 4-[(2R,3S,4R,5S)-3-(3-chloro-2-fluoro-phenyl)-4-(4-chloro-2-fluoro-phenyl)-4-cyano-5-(2,2-dimethyl-propyl)-1-(ethoxycarbonylmethyl-carbamoyl)-pyrrolidine-2-carbonyl]-amino}-3-methoxy-benzoic acid (15.3 mg, 40.8%) as white powder. HRMS (ES+) m/z Calcd for C36H36Cl2F2N4O7+H [(M+H)+]: calc. found. LCMS calc=745.61 found: 745.2. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
58% | In tetrahydrofuran; water; at 85℃; | <strong>[1229705-06-9]4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoic acid</strong> (WO 2010031713 A1, 200 mg, 0.324 mmol) was dissolved in 10 mL of THF. Formaldehyde (Aldrich, 37% in water, 300 mg, 3.7 mmol) was added and the mixture was stirred at 85 C. overnight. The reaction mixture was concentrated to remove some THF. Water was added to form a suspension. The solid was collected by filtration, washed with water and diethyl ether (5 mL) and dried under high vacuum. The solid was dissolved in DMSO and was purified by HPLC (50%-100% ACN/water/TFA). The fractions were combined, concentrated and freeze dried to give a white foam as desired product (118 mg, 58%). LCMS (ES+) m/z Calcd for C32H29Cl2F2N3O4 [(M+H)+]: 628. Found: 628. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
135.4 mg | With caesium carbonate; In N,N-dimethyl-formamide; | Example 8 1-(Cyclohexyloxycarbonyloxy)ethyl 4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoate In a manner similar to the method described in Example 3, 1-chloroethyl chloroformate (Oakwood Products, 1.99 g, 1.5 mL, 13.9 mmol) was reacted with cyclohexanol (Alfa Aesar, 1.33 g, 1.4 mL, 13.3 mmol) and pyridine (1.27 g, 1.3 mL, 16.1 mmol) in methylene chloride (11 mL) at -78 C. for 3 h to give 1-chloroethyl cyclohexyl carbonate. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
134.4 mg | With caesium carbonate; In N,N-dimethyl-formamide; | Example 9 Rac-1-(isopropoxycarbonyloxy)ethyl 4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoate In a manner similar to the method described in Example 3, 1-chloroethyl chloroformate (Oakwood Products, 1.99 g, 1.5 mL, 13.9 mmol) was reacted with 2-propanol (785 mg, 1 mL, 13.0 mmol) and pyridine (1.27 g, 1.3 mL, 16.1 mmol) in methylene chloride (11 mL) at room temperature for 4 h to give 1-chloroethyl isopropyl carbonate. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
With caesium carbonate; In N,N-dimethyl-formamide; at 20℃; | Example 23 1-(2-(Benzyloxy)-2-oxoethylcarbamoyloxy)ethyl 4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoate Benzyl 2-aminoacetate was generated from a treatment of benzyl 2-aminoacetate hydrochloride (Aldrich) in methylene chloride with aqueous sodium bicarbonate solution followed by water wash. To a cooled solution of benzyl 2-aminoacetate (1.837 g, 11.1 mmol) and pyridine (1.1 g, 1.1 mL, 13.9 mmol) in methylene chloride (17 mL) at -78 C., was added slowly 1-chloroethyl chloroformate (Aldrich, 1.67 g, 11.7 mmol) over ?10 min. The reaction mixture was allowed to stir at -78 C. for 3 h, giving a white precipitate. This cold reaction mixture was filtered to remove the solid and the filtrate was concentrated to dryness, giving the crude benzyl 2-((1-chloroethoxy)carbonylamino)acetate which was used for the next step without further purification. To a solution of <strong>[1229705-06-9]4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoic acid</strong> (215 mg, 0.349 mmol) in dimethylformamide (22 mL) was added cesium carbonate (2.15 g, 6.6 mmol). After stirring for 10 min, a solution of the freshly made benzyl 2-((1-chloroethoxy)carbonylamino)acetate (2.77 g crude, ?5.55 mmol) in dimethylformamide (5 mL) was added. The reaction mixture was allowed to stir at room temperature overnight. This reaction mixture was taken up in ethyl acetate, washed with water, brine and concentrated to dryness. The crude material was purified by flash chromatography (hexane/ethyl acetate, 90/10 to 10/90) to give 1-(2-(benzyloxy)-2-oxoethylcarbamoyloxy)ethyl 4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoate as a white solid (191 mg, 64% yield). LCMS (ES+) m/z calcd. for C43H43Cl2F2N4O8 [(M+H)+]: 851, found: 851. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
192 mg | To a solution of chiral <strong>[1229705-06-9]4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoic acid</strong> (prepared as described in US20100152190A1, 200.0 mg, 0.324 mmol) in dimethylformamide (8 mL) was added cesium carbonate (529 mg, 1.62 mmol). The mixture was stirred for 20 min before di-tert-butyl chloromethyl phosphate (TCI, 236 mg, 0.9912 mmol) in dimethylformamide (1 mL) was added. The reaction mixture was allowed to stirr at room temperature for 3 h before it was partitioned between ethyl acetate and water, washed with water and dried over anhydrous sodium sulfate and concentrated. The crude product was purified by flash chromatography (hexane/ethyl acetate, 85/15 to 5/95) to give chiral 4-[(2R,3S,4R,5S)-3-(3-chloro-2-fluoro-phenyl)-4-(4-chloro-2-fluoro-phenyl)-4-cyano-5-(2,2-dimethyl-propyl)-pyrrolidine-2-carbonyl]-amino}-3-methoxy-benzoic acid di-tert-butoxy-phosphoryloxymethyl ester as a white solid (192 mg, 70% yield). LCMS (ES+) m/z calcd. for C40H49Cl2F2N3O8P [(M+H)+]: 838, found: 838. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
127 mg | To a solution of <strong>[1229705-06-9]4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoic acid</strong> (prepared as described in US20100152190A1, 100 mg, 162 mumol) in dry dimethyl formamide (4 mL) was added cesium carbonate (185 mg, 568 mumol). The mixture was stirred for 10 min at room temperature. The freshly made solution of 1-chloroethyl (3aS,5S,6R,6aS)-5-((S)-2,2-dimethyl-1,3-dioxolan-4-yl)-2,2-dimethyltetrahydrofuro[3,2-d][1,3]dioxol-6-yl carbonate (178 mg, 487 mumol) in 4 mL of dry dimethyl formamide was added to the above mixture and stirred at room temperature overnight. It was diluted with ethyl acetate and washed with water (2*) and brine. The organic layer was dried over anhydrous sodium sulfate, filtered and concentrated to give a crude, which was purified with flash chromatography (0-50% ethyl acetate in hexane over 25 min) to give an off-white solid (127 mg, 82% yield). LCMS (ES+) m/z calcd. for C46H51Cl2F2N3O12 [(M+H)+]: 946, found: 946. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
168 mg | To a solution of chiral <strong>[1229705-06-9]4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoic acid</strong> (US20100152190A1, 200 mg, 0.324 mmol) in dimethylformamide (8 mL) was added cesium carbonate (846 mg, 2.6 mmol). After stirring for 10 min, a solution of the above freshly made 1-chloroethyl bis(2-methoxyethyl)carbamate (510 mg, 2.13 mmol) in dimethylformamide (3 mL) was added. The reaction mixture was allowed to stir at room temperature overnight. This reaction mixture was taken up in ethyl acetate, washed with water, brine and concentrated to dryness. The crude material was purified by flash chromatography (hexane/ethyl acetate, 90/10 to 10/90) to give 4-[(2R,3S,4R,5S)-3-(3-chloro-2-fluoro-phenyl)-4-(4-chloro-2-fluoro-phenyl)-4-cyano-5-(2,2-dimethyl-propyl)-pyrrolidine-2-carbonyl]-amino}-3-methoxy-benzoic acid 1-[bis-(2-methoxy-ethyl)-carbamoyloxy]-ethyl ester as a white solid (168 mg, 63% yield). LCMS (ES+) m/z calcd. for C40H47Cl2F2N4O8 [(M+H)+]: 819, found: 819. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
With caesium carbonate; In N,N-dimethyl-formamide; | 4-Methyl-piperazine-1-carboxylic acid 1-(4-[(2R,3S,4R,5S)-3-(3-chloro-2-fluoro-phenyl)-4-(4-chloro-2-fluoro-phenyl)-4-cyano-5-(2,2-dimethyl-propyl)-pyrrolidine-2-carbonyl]-amino}-3-methoxy-benzoyloxy)-ethyl ester [0112] [0113] In a manner similar to the method described in Example 3, 1-chloroethyl carbonochloridate was reacted with 1-methylpiperazine and pyridine in methylene chloride at -78 C. for 3 h to give 1-chloroethyl 4-methylpiperazine-1-carboxylate hydrochloride salt which was reacted with chiral <strong>[1229705-06-9]4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoic acid</strong> in the presence of cesium carbonate in dimethylformamide overnight to give, after flash chromatography purification (hexane/ethyl acetate, 80/20 to 20/80), 4-methyl-piperazine-1-carboxylic acid 1-(4-[(2R,3S,4R,5S)-3-(3-chloro-2-fluoro-phenyl)-4-(4-chloro-2-fluoro-phenyl)-4-cyano-5-(2,2-dimethyl-propyl)-pyrrolidine-2-carbonyl]-amino}-3-methoxy-benzoyloxy)-ethyl ester as a white solid. LCMS (ES+) m/z calcd. for C39H44Cl2F2N5O6 [(M+H)+]: 786, found: 786. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
198.8 mg | With caesium carbonate; In dichloromethane; N,N-dimethyl-formamide; at 20℃; for 3h; | Example 6 Rac-1-(isobutyryloxy)ethyl 4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoate In a manner similar to the method described in Example 5, paraldehyde (JT-Baker, 1.37 g, 10.4 mmol) was reacted with isobutyryl chloride (Aldrich, 2.93 g, 27.5 mmol) and sodium iodide (4.9472 g, 33.0 mmol) in methylene chloride (100 mL) at room temperature for 16 h to give 1-iodoethyl isobutyrate. A portion of this material (400.2 mg, 1.65 mmol) was then reacted with chiral <strong>[1229705-06-9]4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoic acid</strong> (200.8 mg, 0.332 mmol) and cesium carbonate (653.2 mg, 2.00 mmol) in dimethylformamide (6 mL) at room temperature for 3 h to give 1-(isobutyryloxy)ethyl 4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoate as an off-white solid (198.8 mg, 83% yield). MS (ES+) m/z calcd. for C37H40Cl2F2N3O6: [(M+H)+]: 730, found: 730 |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
97.7 mg | With caesium carbonate; In N,N-dimethyl-formamide; | Example 15 <strong>[1229705-06-9]4-[(2R,3S,4R,5S)-4-(4-Chloro-2-fluoro-phenyl)-3-(3-chloro-2-fluoro-phenyl)-4-cyano-5-(2,2-dimethyl-propyl)-pyrrolidine-2-carbonyl]-amino}-3-methoxy-benzoic acid</strong> 1-(2,2-dimethyl-[1,3]dioxolan-4-ylmethylcarbamoyloxy)-ethyl ester In a manner similar to the method described in Example 3, 1-chloroethyl chloroformate (1.46 g, 1.1 mL, 10.2 mmol) was reacted with (2,2-dimethyl-[1,3]-dioxolan-4-yl)-methylamine (Aldrich, 1.21 g, 1.2 mL, 8.98 mmol) and pyridine (870 mg, 0.89 mL, 11.0 mmol) in methylene chloride (15 mL) at 78 C. for 3 h to give 1-chloroethyl (2,2-dimethyl-1,3-dioxolan-4-yl)methylcarbamate with pyridine hydrochloride (1:1). A portion of this material (622 mg, 1.76 mmol) in dimethylformamide (5 mL) was then reacted with chiral <strong>[1229705-06-9]4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoic acid</strong> (201 mg, 0.326 mmol) in the presence of cesium carbonate (806.9 mg, 2.48 mmol) in dimethylformamide (7 mL) overnight to give 4-[(2R,3S,4R,5S)-4-(4-chloro-2-fluoro-phenyl)-3-(3-chloro-2-fluoro-phenyl)-4-cyano-5-(2,2-dimethyl-propyl)-pyrrolidine-2-carbonyl]-amino}-3-methoxy-benzoic acid 1-(2,2-dimethyl-[1,3]dioxolan-4-ylmethylcarbamoyloxy)-ethyl ester as a white solid (97.7 mg, 36% yield). MS (ES+) m/z calcd. for C40H45C12F2N4O8: [(M+H)+]: 817, found: 817 |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
145.7 mg | With caesium carbonate; In N,N-dimethyl-formamide; at 20℃; for 17h; | Example 17 1-(Tetrahydro-2H-pyran-4-ylcarbamoyloxy)ethyl 4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoate In a manner similar to the method described in Example 3, 1-chloroethyl chloroformate (Oakwood, 1.32 g, 1.0 mL, 9.27 mmol) was reacted with 4-aminotetrahydropyrane (Oakwood, 899 mg, 0.92 mL, 8.62 mmol) and pyridine (782 mg, 0.8 mL, 9.89 mmol) in methylene chloride (20 mL) at -78 C. for 3 h to give 1-chloroethyl tetrahydro-2H-pyran-4-ylcarbamate compound with pyridine hydrochloride (1:1). A portion of this material (520 mg, 1.61 mmol) in dimethylformamide (5 mL) was then reacted with chiral <strong>[1229705-06-9]4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoic acid</strong> (151.4 mg, 0.246 mmol) and cesium carbonate (599.5 mg, 1.84 mmol) in dimethylformamide (4.00 mL) at room temperature for 17 h to give 1-(tetrahydro-2H-pyran-4-ylcarbamoyloxy)ethyl 4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoate as a white solid (145.7 mg, 75% yield). MS (ES+) m/z calcd. for C39H42Cl2F2N4O7: [(M+H)+]: 787, found: 787 |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
With caesium carbonate; In N,N-dimethyl-formamide; | Example 18 4-[(2R,3S,4R,5S)-3-(3-Chloro-2-fluoro-phenyl)-4-(4-chloro-2-fluoro-phenyl)-4-cyano-5-(2,2-dimethyl-propyl)-pyrrolidine-2-carbonyl]-amino}-3-methoxy-benzoic acid 1-{2-[2-(2-methoxy-ethoxy)-ethoxy]-ethoxycarbonyloxy}-ethyl ester [0142] [0143] In a manner similar to the method described in Example 3, 1-chloroethyl chloroformate was reacted with 2-(2-(2-methoxyethoxy)ethoxy)ethanol (TCI) and pyridine in methylene chloride at -78 C. for 3 h to give 1-chloroethyl 2-(2-(2-methoxyethoxy)ethoxy)ethyl carbonate. This was then reacted with chiral <strong>[1229705-06-9]4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoic acid</strong> in the presence of cesium carbonate in dimethylformamide overnight to give, after flash chromatography purification (hexane/ethyl acetate, 80/20 to 20/80), 4-[(2R,3S,4R,5S)-3-(3-chloro-2-fluoro-phenyl)-4-(4-chloro-2-fluoro-phenyl)-4-cyano-5-(2,2-dimethyl-propyl)-pyrrolidine-2-carbonyl]-amino}-3-methoxy-benzoic acid 1-{2-[2-(2-methoxy-ethoxy)-ethoxy]-ethoxycarbonyloxy}-ethyl ester as a white solid. LCMS (ES+) m/z calcd. for C41H48Cl2F2N3O10 [(M+H)+]: 850, found: 850. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
With caesium carbonate; In N,N-dimethyl-formamide; | Example 19 4-[(2R,3S,4R,5S)-3-(3-Chloro-2-fluoro-phenyl)-4-(4-chloro-2-fluoro-phenyl)-4-cyano-5-(2,2-dimethyl-propyl)-pyrrolidine-2-carbonyl]-amino}-3-methoxy-benzoic acid 1-(2-{2-[2-(2-methoxy-ethoxy)-ethoxy]-ethoxy}-ethoxycarbonyloxy)-ethyl ester [0144] [0145] In a manner similar to the method described in Example 3, 1-chloroethyl chloroformate was reacted with 2,5,8,11-tetraoxamidecan-13-ol (TCI) and pyridine in methylene chloride at -78 C. for 3 h to give 1-chloroethyl 2,5,8,11-tetraoxamidecan-13-yl carbonate. This was then reacted with <strong>[1229705-06-9]4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoic acid</strong> in the presence of cesium carbonate in dimethylformamide overnight to give, after flash chromatography purification (hexane/ethyl acetate, 80/20 to 20/80), 4-[(2R,3S,4R,5S)-3-(3-chloro-2-fluoro-phenyl)-4-(4-chloro-2-fluoro-phenyl)-4-cyano-5-(2,2-dimethyl-propyl)-pyrrolidine-2-carbonyl]-amino}-3-methoxy-benzoic acid 1-(2-{2-{2-(2-methoxy-ethoxy)-ethoxy]-ethoxy}-ethoxycarbonyloxy)ethyl ester as a white solid. LCMS (ES+) m/z calcd. for C43H52Cl2F2N3O11 [(M+H)+]: 894, found: 894. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
With caesium carbonate; In N,N-dimethyl-formamide; | Example 20 4-[(2R,3S,4R,5S)-3-(3-Chloro-2-fluoro-phenyl)-4-(4-chloro-2-fluoro-phenyl)-4-cyano-5-(2,2-dimethyl-propyl)-pyrrolidine-2-carbonyl]-amino}-3-methoxy-benzoic acid 1-[2-(2-{2-[2-(2-methoxy-ethoxy)-ethoxy]-ethoxy}-ethoxy)-ethoxycarbonyloxy]-ethyl ester [0146] [0147] In a manner similar to the method described in Example 3, 1-chloroethyl chloroformate was reacted with pentaethylene glycol monomethyl ether (TCI) and pyridine in methylene chloride at -78 C. for 3 h to give 1-chloroethyl 2,5,8,11,14-pentaoxahexadecan-16-yl carbonate. This was then reacted with <strong>[1229705-06-9]4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoic acid</strong> in the presence of cesium carbonate in dimethylformamide overnight to give, after flash chromatography purification (hexane/ethyl acetate, 80/20 to 20/80), 4-[(2R,3S,4R,5S)-3-(3-chloro-2-fluoro-phenyl)-4-(4-chloro-2-fluoro-phenyl)-4-cyano-5-(2,2-dimethyl-propyl)-pyrrolidine-2-carbonyl]-amino}-3-methoxy-benzoic acid 1-[2-(2-{2-[2-(2-methoxy-ethoxy)-ethoxy]-ethoxy}-ethoxy)-ethoxycarbonyloxy]-ethyl ester as a white solid. LCMS (ES+) m/z calcd. for C45H56Cl2F2N3O12 [(M+H)+]: 938, found: 938. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
With caesium carbonate; In N,N-dimethyl-formamide; | Example 21 21-oxo-2,5,8,11,14,17,20,22-octaoxatetracosan-23-yl 4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoate [0148] [0149] In a manner similar to the method described in Example 3, 1-chloroethyl chloroformate was reacted with 2,5,8,11,14,17-hexaoxanonadecan-19-ol (TCI) and pyridine in methylene chloride at -78 C. for 3 h to give 1-chloroethyl 2,5,8,11,14,17-hexaoxanonadecan-19-yl carbonate. This was then reacted with <strong>[1229705-06-9]4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoic acid</strong> in the presence of cesium carbonate in dimethylformamide overnight to give, after flash chromatography purification (hexane/ethyl acetate, 80/20 to 20/80), 21-oxo-2,5,8,11,14,17,20,22-octaoxatetracosan-23-yl-4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoate as a white solid. LCMS (ES+) m/z calcd. for C47H60Cl2F2N3O13 [(M+H)+]: 982, found: 982. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
With caesium carbonate; In N,N-dimethyl-formamide; | Example 25 (2S)-Dibenzyl 2-((1-(4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoyloxy)ethoxy)-carbonylamino)pentanedioate In a manner similar to the method described in Example 23, chloromethoxycarbonylamino-acetic acid benzyl ester was prepared from amino-acetic acid benzyl ester and 1-chloroethyl chloroformate (Aldrich) in the presence of pyridine in methylene chloride. It was then reacted with chiral <strong>[1229705-06-9]4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoic acid</strong> and cesium carbonate in dimethylformamide to give chiral 4-[(2R,3S,4R,5S)-3-(3-chloro-2-fluoro-phenyl)-4-(4-chloro-2-fluoro-phenyl)-4-cyano-5-(2,2-dimethyl-propyl)-pyrrolidine-2-carbonyl]-amino}-3-methoxy-benzoic acid benzyloxycarbonylmethyl-carbamoyloxymethyl ester as a white solid. MS (ES+) m/z calcd. for C42H41Cl2F2N4O8 [(M+H)+]: 837, found: 837 |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
With caesium carbonate; In N,N-dimethyl-formamide; | Example 31 (S)-Dibenzyl 2-(((4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoyloxy)methoxy)carbonylamino)-pentanedioate In a manner similar to the method described in Example 23, (S)-dibenzyl 2-((chloromethoxy)-carbonylamino)pentanedioate was prepared from (S)-dibenzyl 2-aminopentanedioate and chloromethyl chloroformate in the presence of pyridine in methylene chloride. It was then reacted with chiral <strong>[1229705-06-9]4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoic acid</strong> and cesium carbonate in dimethylformamide to give chiral (S)-dibenzyl 2-(((4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoyloxy)methoxy)carbonylamino)-pentanedioate as a white solid. MS (ES+) m/z calcd. for C52H51Cl2F2N4O10 [(M+H)+]: 999, found: 999. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
With caesium carbonate; In N,N-dimethyl-formamide; | Example 33 15-methyl-12-oxo-2,5,8,11,13-pentaoxahexadecan-14-yl 4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoate [0165] [0166] In a manner similar to the method described in Example 3, 1-chloro-2-methylpropyl chloroformate (Aldrich) was reacted with 2-(2-(2-methoxyethoxy)ethoxy)ethanol (TCI) and pyridine in methylene chloride at -78 C. for 3 h to give 1-chloro-2-methylpropyl 2-(2-(2-methoxyethoxy)-ethoxy)ethyl carbonate. This material was then reacted with chiral <strong>[1229705-06-9]4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoic acid</strong> in the presence of cesium carbonate in dimethylformamide overnight to give, after flash chromatography purification (hexane/ethyl acetate, 80/20 to 20/80), 15-methyl-12-oxo-2,5,8,11,13-pentaoxahexadecan-14-yl 4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoate as a white solid. MS (ES+) m/z calcd. for C43H52Cl2F2N3O10: [(M+H)+]: 878, found: 878. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
With caesium carbonate; In N,N-dimethyl-formamide; | Example 34 3-Oxo-2,4,7,10,13-pentaoxatetradecyl 4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoate [0167] [0168] In a manner similar to the method described in Example 3, chloromethyl chloroformate (Aldrich) was reacted with 2-(2-(2-methoxyethoxy)ethoxy)ethanol (TCI) and pyridine in methylene chloride at -78 C. for 3 h to give chloromethyl 2-(2-(2-methoxyethoxy)ethoxy)ethyl carbonate. This material was then reacted with chiral <strong>[1229705-06-9]4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoic acid</strong> in the presence of cesium carbonate in dimethylformamide overnight to give, after flash chromatography purification (hexane/ethyl acetate, 80/20 to 20/80), chiral 3-oxo-2,4,7,10,13-pentaoxatetradecyl 4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoate as a white solid. MS (ES+) m/z calcd. for C40H47Cl2F2N3O10: [(M+H)+]: 836, found: 836. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
With caesium carbonate; In N,N-dimethyl-formamide; | Example 35 3-Oxo-2,4,7,10,13,16,19-heptaoxaicosyl 4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoate [0169] [0170] In a manner similar to the method described in Example 3, chloromethyl chloroformate (Aldrich) was reacted with pentaethylene glycol monomethyl ether (TCI) and pyridine in methylene chloride at -78 C. for 3 h to give chloromethyl 2,5,8,11,14-pentaoxahexadecan-16-yl carbonate. This material was then reacted with chiral <strong>[1229705-06-9]4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoic acid</strong> in the presence of cesium carbonate in dimethylformamide overnight to give, after flash chromatography purification (hexane/ethyl acetate, 80/20 to 20/80), chiral 3-oxo-2,4,7,10,13,16,19-heptaoxaicosyl 4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoate as a white solid. MS (ES+) m/z calcd. for C44H54Cl2F2N3O12: [(M+H)+]: 924, found: 924. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
With caesium carbonate; In N,N-dimethyl-formamide; | Example 39 3-Oxo-2,4,7,10,13-pentaoxatetradecyl 4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoate [0179] [0180] In a manner similar to the method described in Example 3, chloromethyl chloroformate (Aldrich) was reacted with 2,5,8,11,14,17-hexaoxanonadecan-19-ol (TCI) and pyridine in methylene chloride at -78 C. for 3 h to give chloromethyl 2,5,8,11,14,17-hexaoxanonadecan-19-yl carbonate. This material was then reacted with chiral <strong>[1229705-06-9]4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoic acid</strong> in the presence of cesium carbonate in dimethylformamide overnight to give, after flash chromatography purification (hexane/ethyl acetate, 80/20 to 20/80), chiral-3-oxo-2,4,7,10,13-pentaoxatetradecyl 4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoate as a white solid. MS (ES+) m/z calcd. for C46H58Cl2F2N3O13: [(M+H)+]: 968, found: 968. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
With caesium carbonate; In N,N-dimethyl-formamide; | Example 40 [0181] 27-Oxo-2,5,8,11,14,17,20,23,26,28-decaoxatriacontan-29-yl-4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoate [0182] In a manner similar to the method described in Example 3, 1-chloroethyl chloroformate (Aldrich) was reacted with octaethylene glycol monomethyl ether (TCI) and pyridine in methylene chloride at -78 C. for 3 h to give 1-chloroethyl 2,5,8,11,14,17,20,23-octaoxapentacosan-25-yl carbonate. This was then reacted with chiral <strong>[1229705-06-9]4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoic acid</strong> in the presence of cesium carbonate in dimethylformamide overnight to give, after flash chromatography purification (hexane/ethyl acetate, 80/20 to 20/80), 27-oxo-2,5,8,11,14,17,20,23,26,28-decaoxatriacontan-29-yl-4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoate as a white solid. MS (ES+) m/z calcd. for C51H68Cl2F2N3O15: [(M+H)+]: 1071, found: 1070. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
With caesium carbonate; In N,N-dimethyl-formamide; | Example 41 [0183] 24-Oxo-2,5,8,11,14,17,20,23,25-nonaoxaheptacosan-26-yl 4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoate [0184] In a manner similar to the method described in Example 3, 1-chloroethyl chloroformate (Aldrich) was reacted with heptaethylene glycol monomethyl ether (TCI) and pyridine in methylene chloride at -78 C. for 3 h to give 1-chloroethyl 2,5,8,11,14,17,20,23-octaoxapentacosan-25-yl carbonate. This was then reacted with chiral <strong>[1229705-06-9]4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoic acid</strong> in the presence of cesium carbonate in dimethylformamide overnight to give, after flash chromatography purification (hexane/ethyl acetate, 80/20 to 20/80), 24-oxo-2,5,8,11,14,17,20,23,25-nonaoxaheptacosan-26-yl-4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoate as a white solid. MS (ES+) m/z calcd. for C51H68Cl2F2N3O15: [(M+H)+]: 1026, found: 1026. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
With caesium carbonate; In N,N-dimethyl-formamide; | Example 48 (S)-2-(((4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoyloxy)methoxy)carbonylamino)propanoic acid In a manner similar to the method described in Example 23, (S)-benzyl 2-((chloromethoxy)-carbonylamino)propanoate was prepared from (S)-benzyl 2-aminopropanoate (Chem-Impex) and 1-chloromethyl chloroformate (Aldrich) in the presence of pyridine in methylene chloride. It was then reacted with chiral <strong>[1229705-06-9]4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoic acid</strong> and cesium carbonate in dimethylformamide to give chiral ((S)-1-(benzyloxy)-1-oxopropan-2-ylcarbamoyloxy)methyl 4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoate as a white solid. In a manner similar to the method described in Example 24, a solution of chiral ((S)-1-(benzyloxy)-1-oxopropan-2-ylcarbamoyloxy)methyl 4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoate in ethyl acetate was treated with 10% palladium on carbon under 1 atm of hydrogen to give chiral (S)-2-(((4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoyloxy)methoxy)carbonylamino)propanoic acid as a white solid. MS (ES+) m/z calcd. for C36H36Cl2F2N4O8: [(M+H)+]: 761, found: 761. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
With caesium carbonate; In N,N-dimethyl-formamide; | Example 49 Dibenzyl <strong>[1229705-06-9]4-[(2R,3S,4R,5S)-4-(4-Chloro-2-fluoro-phenyl)-3-(3-chloro-2-fluoro-phenyl)-4-cyano-5-(2,2-dimethyl-propyl)-pyrrolidine-2-carbonyl]-amino}-3-methoxy-benzoic acid</strong> 2-{2-[2-(2-phosphonooxy-ethoxy)-ethoxy]-ethoxy}-ethoxycarbonyloxymethyl ester In a manner similar to the method described in Example 3, chloromethyl chloroformate (Oakwood) was reacted with dibenzyl 2-(2-(2-(2-hydroxyethoxy)ethoxy)ethoxy)ethyl phosphate (Example 54) and pyridine in methylene chloride at -78 C. for 3 h to give the corresponding chloromethyl carbonate with pyridine hydrochloride (1:1). This was then reacted with chiral <strong>[1229705-06-9]4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoic acid</strong> in the presence of cesium carbonate in dimethylformamide overnight to give, after flash chromatography purification, chiral dibenzyl 4-[(2R,3S,4R,5S)-4-(4-chloro-2-fluoro-phenyl)-3-(3-chloro-2-fluoro-phenyl)-4-cyano-5-(2,2-dimethyl-propyl)-pyrrolidine-2-carbonyl]-amino}-3-methoxy-benzoic acid 2-{2-[2-(2-phosphonooxy-ethoxy)-ethoxy]-ethoxy}-ethoxycarbonyloxymethyl ester. MS (ES+) m/z calcd. for C55H61Cl2F2N3O14P: [(M+H)+]: 1126, found: 1126. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
102 mg | To a solution of <strong>[1229705-06-9]4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoic acid</strong> (prepared as described in US20100152190A1, 136 mg, 221 mumol) in dry dimethyl formamide (10 mL) was added cesium carbonate (359 mg, 1.1 mmol). It was stirred at room temperature for 10 min. Carbonic acid 2-(2-{2-[2-(bis-benzyloxy-phosphoryloxy)-ethoxy]-ethoxy}-ethoxy)-ethyl ester 1-chloro-ethyl ester, the thick yellow oil got from previous step (617 mg, 1.1 mmol), dissolved in 3 mL of dry dimethyl formamide was added to the above mixture and stirred at room temperature overnight. The reaction was completed as shown by LC-MS. The reaction mixture was poured into water. Then it was extracted with ethyl acetate (2×). The ethyl acetate layers were combined and washed with water and brine. It was dried over anhydrous sodium sulfate, filtered and concentrated to give a crude, which was purified with flash chromatography (30-100% ethyl acetate in hexane over 30 min) to give 4-[(2R,3S,4R,5S)-3-(3-chloro-2-fluoro-phenyl)-4-(4-chloro-2-fluoro-phenyl)-4-cyano-5-(2,2-dimethyl-propyl)-pyrrolidine-2-carbonyl]-amino}-3-methoxy-benzoic acid 1-(2-{2-[2-(2-dibenzyloxyphosphoryloxy-ethoxy)-ethoxy]-ethoxy}-ethoxycarbonyloxy)-ethyl ester as a clear oil (102 mg, 40.5% yield). LCMS (ES+) m/z calcd. for C56H62Cl2F2N3O14P [(M+H)+]: 1140, found: 1140. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
149.4 mg | With caesium carbonate; sodium iodide; In dichloromethane; at 20℃; for 16h; | Example 5 Acetoxyethyl 4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoate A mixture of paraldehyde (JT-Baker, 1.39 g, 1.4 mL, 10.5 mmol) and sodium iodide (5.01 g, 33.4 mmol) in methylene chloride (75 mL) were treated with acetyl chloride (2.21 g, 2 mL, 27.8 mmol) in methylene chloride (25 mL) over 20 min and allowed to stir at room temperature in the dark for 18 h. The reaction mixture was filtered and concentrated to give 1-iodoethyl acetate as a brown oil. To a mixture of the above 1-iodoethyl acetate (311 mg, 1.457 mmol) and chiral <strong>[1229705-06-9]4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoic acid</strong> (US20100152190A1, 201.7 mg, 0.327 mmol) in dimethylformamide (6 mL) was added cesium carbonate (659 mg, 2.00 mmol) and the mixture was stirred at room temperature for 16 h. The reaction mixture was taken up in ethyl acetate, washed with water and then brine. The organic layer was dried over sodium sulfate and concentrated to dryness. The crude material was purified by flash chromatography (hexane/ethyl acetate, 80/20 to 20/80) to give 1-acetoxyethyl 4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoate (149.4 mg, 65% yield). MS (ES+) m/z calcd. for C35H36Cl2F2N3O6: [(M+H)+]: 702.19, found: 702.4 |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
42.8 mg | With caesium carbonate; In N,N-dimethyl-formamide;Darkness; | Example 7 <strong>[1229705-06-9]4-[(2R,3S,4R,5S)-4-(4-Chloro-2-fluoro-phenyl)-3-(3-chloro-2-fluoro-phenyl)-4-cyano-5-(2,2-dimethyl-propyl)-pyrrolidine-2-carbonyl]-amino}-3-methoxy-benzoic acid</strong> acetoxymethyl ester A mixture of 1,3,5-trioxane (Aldrich, 970.8 mg, 10.8 mmol) and sodium iodide (5.12 g, 34.2 mmol) in methylene chloride (100 mL) was treated with acetyl chloride (2.26 g, 2.1 mL, 28.5 mmol) and stirred at room temperature in the dark for 16 h. The reaction mixture was filtered and concentrated to give crude iodomethyl acetate as a brown oil. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
186.5 mg | With caesium carbonate; In N,N-dimethyl-formamide; | Example 10 1-(4-((2R,3S,4R,5S)-3-(3-Chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoyloxy)ethyl morpholine-4-carboxylate In a manner similar to the method described in Example 3, 1-chloroethyl chloroformate (2.64 g, 2 mL, 18.5 mmol) was reacted with morpholine (1.69 g, 1.7 mL, 19.4 mmol) and pyridine (1.68 g, 1.72 mL, 21.2 mmol) in methylene chloride (25 mL) at room temperature overnight to give the crude product, 1-chloroethyl morpholine-4-carboxylate. A portion of 1-chloroethyl morpholine-4-carboxylate (670.1 mg, 3.46 mmol) was then reacted with chiral <strong>[1229705-06-9]4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoic acid</strong> (200.5 mg, 0.325 mmol) in the presence of cesium carbonate (1.094 g, 3.36 mmol) in dimethylformamide (7 mL) overnight to give, 1-(4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoyloxy)ethyl morpholine-4-carboxylate as an off-white solid (186.5 mg, 74% yield). MS (ES+) m/z calcd. for C38H41Cl2F2N4O7: [(M+H)+]: 773, found: 773 |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
259.9 mg | With caesium carbonate; In N,N-dimethyl-formamide; | Example 12 Rac-1-tert-butyl 4-(1-(4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoyloxy)ethyl)piperazine-1,4-dicarboxylate [0130] 1-Boc-piperazine (Alfa Aesar, 1.5756 g, 8.37 mmol) and pyridine (870 mg, 0.89 mL, 11.0 mmol) in methylene chloride (10 mL) from -78 C. for 1 h and then at room temperature overnight to give piperazine-1,4-dicarboxylic acid tert-butyl ester 1-chloro-ethyl ester. A portion of piperazine-1,4-dicarboxylic acid tert-butyl ester 1-chloro-ethyl ester (682 mg, 1.67 mmol) was then reacted with chiral <strong>[1229705-06-9]4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoic acid</strong> (201.4 mg, 0.327 mmol) in the presence of cesium carbonate (743.4 mg, 2.28 mmol) in dimethylformamide (7 mL) overnight to give 1-tert-butyl 4-(1-(4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoyloxy)ethyl)piperazine-1,4-dicarboxylate as an off-white lyophilized solid (259.9 mg, 91% yield). MS (ES+) m/z calcd. for C34H36Cl2F2N4O4: [(M+H)+]: 872, found: 872 |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
175.3 mg | With caesium carbonate; In N,N-dimethyl-formamide; | Example 14 rac-1,1-Dioxo-thiomorpholine-4-carboxylic acid 1-(4-[(2R,3S,4R,5S)-4-(4-chloro-2-fluoro-phenyl)-3-(3-chloro-2-fluoro-phenyl)-4-cyano-5-(2,2-dimethyl-propyl)-pyrrolidine-2-carbonyl]-amino}-3-methoxy-benzoyloxy)-ethyl ester In a manner similar to the method described in Example 3, 1-chloroethyl chloroformate (Oakwood, 1.32 g, 1 mL, 9.27 mmol) was reacted with thiomorpholine 1,1-dioxide (1.1638 g, 8.61 mmol) and pyridine (783 mg, 801 mul, 9.9 mmol) in methylene chloride (15 mL) at room temperature for 3 h to give 1,1-dioxo-thiomorpholine-4-carboxylic acid 1-chloro-ethyl ester. A portion of 1,1-dioxo-thiomorpholine-4-carboxylic acid 1-chloro-ethyl ester (577 mg, 1.614 mmol) was then reacted with chiral <strong>[1229705-06-9]4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoic acid</strong> (150.5 mg, 0.244 mmol) in the presence of cesium carbonate (598.4 mg, 1.84 mmol) in dimethylformamide (7 mL) overnight to give 1,1-dioxo-thiomorpholine-4-carboxylic acid 1-(4-[(2R,3S,4R,5S)-4-(4-chloro-2-fluoro-phenyl)-3-(3-chloro-2-fluoro-phenyl)-4-cyano-5-(2,2-dimethyl-propyl)-pyrrolidine-2-carbonyl]-amino}-3-methoxy-benzoyloxy)-ethyl ester as an off-white solid (175.3 mg, 87% yield). MS (ES+) m/z calcd. for C38H41Cl2F2N4O8S: [(M+H)+]: 821, found: 821 |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
102 mg | With caesium carbonate; In N,N-dimethyl-formamide; at 20℃; | To a solution of chiral <strong>[1229705-06-9]4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoic acid</strong> (prepared as described in US20100152190A1, 150 mg, 0.243 mmol) in dimethylformamide (6 mL) was added cesium carbonate (243 mg, 0.746 mmol). After stirring for a few minutes, a solution of the above freshly made ethyl-isopropyl-carbamic acid 1-chloro-ethyl ester (190 mg, 0.491 mmol) in dry dimethylformamide (1 mL) was added and the reaction mixture was allowed to stir at room temperature overnight. This reaction mixture was diluted with ethyl acetate and washed with water, brine and concentrated to dryness. The crude material was purified by chromatography (hexane/ethyl acetate, 90/10 to 10/90) to give 1-(ethyl(isopropyl)-carbamoyloxy)ethyl 4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoate as a white solid (102 mg, 54% yield). LCMS (ES+) m/z calcd. for C39H45Cl2F2N4O6 [(M+H)-]: 773, found: 773. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
86% | A mixture of chiral <strong>[1229705-06-9]4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoic acid</strong> (100.2 mg, 0.164 mmol) and N,N'-carbonyldiimidazole (68.8 mg, 0.424 mmol, Aldrich) in tetrahydrofuran (5 mL) was stirred at room temperature overnight. N,N-dimethylethanolamine (44.3 mg, 0.495 mmol, Aldrich) was added to a suspension of sodium hydride (12 mg, 0.475 mmol) in tetrahydrofuran (4 mL) and stirred at room temperature for 1 h. It was then added to the above mixture and stirred at room temperature for 1 h. The reaction mixture was quenched with water and extracted with ethyl acetate. The organic layer was washed with water, brine and dried over anhydrous sodium sulfate and concentrated. the crude product was purified by flash chromatography to give chiral 2-(dimethylamino)ethyl 4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoate as a white solid (97.2 mg, 86% yield). MS (ES+) m/z calcd. for C35H39Cl2F2N4O4[(M+H)+]: 687. found: 687 |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
23% | With bis-(2-oxo-3-oxazolidinyl)phosphoryl chloride; triethylamine; In dichloromethane; at 20℃; | To a suspension of chiral <strong>[1229705-06-9]4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoic acid</strong> (150 mg, 0.243 mmol) in methylene chloride (8 mL) was added triethylamine (72.0 mg, 0.10 mL, 0.717 mmol) followed by bis(2-oxo-3-oxazolidinyl)phosphinic chloride (Aldrich, 92 mg, 0.365 mmol). The mixture was stirred at room temperature for 10 min before 2,2'-oxydiethanol (Aldrich, 154 mg, 0.14 mL, 1.45 mmol) was added. This reaction mixture was stirred at room temperature overnight. It was then diluted with methylene chloride and washed with water, brine and concentrated to dryness. The crude material was purified by flash chromatography (eluting with hexane/ethyl acetate, 80/20 to 10/90) to give chiral 2-(2-hydroxyethoxy)ethyl 4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoate as a white solid (40.8 mg, 23% yield). MS (ES+) m/z calcd. for C35H38Cl2F2N3O6[(M+H)+]: 704. found: 704 |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
81% | In a 15 mL pressure tube, <strong>[1229705-06-9]4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoic acid</strong> (prepared as described in US20100152190A1, 100 mg, 162 mumol), 2-chloro-1-(4-methylpiperazin-1-yl)ethanone, hydrochloride (34.6 mg, 162 mumol) and cesium carbonate (111 mg, 341 mumol) were combined with dry dimethyl formamide (2 mL) to give a white suspension. The tube was capped and heated at 50 C. with stirring. After 1.5 h, the reaction mixture was cooled down to room temperature. It was poured into water and extracted with methylene chloride (2*). The methylene chloride layers were combined, dried over anhydrous sodium sulfate, filtered and concentrated. The residue was purified by flash chromatography (24 g pre-packed silica gel column, eluted with methanol in methylene chloride) to give 4-[(2R,3S,4R,5S)-4-(4-chloro-2-fluoro-phenyl)-3-(3-chloro-2-fluoro-phenyl)-4-cyano-5-(2,2-dimethyl-propyl)-pyrrolidine-2-carbonyl]-amino}-3-methoxy-benzoic acid 2-(4-methyl-piperazin-1-yl)-2-oxo-ethyl ester, hydrochloride as an off-white solid (99 mg, 81% yield). The product was mixed with acetonitrile (2 mL) to form a suspension. A few drops of 1N aqueous hydrochloric acid were added to the mixture. The suspension became clear solution. It was frozen and lyophilized to give an off-white solid as hydrochloride salt. LCMS (ES+) m/z calcd. for C38H42Cl2F2N5O5[(M+H)+]: 756. found: 756 |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
58% | With caesium carbonate; In N,N-dimethyl-formamide; at 50℃;Sealed tube; | In a 15 mL pressure tube, <strong>[1229705-06-9]4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoic acid</strong> (prepared as described in US20100152190A1, 100 mg, 162 mumol) and cesium carbonate (63.4 mg, 195 mumol) were combined with dimethyl formamide (2 mL). 2-Chloroacetamide (15.2 mg, 162 mumol) was added to the mixture with stirring to give a white suspension. The tube was capped and heated at 50 C. overnight. The reaction mixture was cooled down to room temperature. It was poured into water and extracted with ethyl acetate (2*). The ethyl acetate layers were combined, dried over anhydrous sodium sulfate, filtered and concentrated. The residue was purified with flash chromatography (24 g pre-packed silica gel column, eluted with ethyl acetate in hexanes) to give <strong>[1229705-06-9]4-[(2R,3S,4R,5S)-4-(4-chloro-2-fluorophenyl)-3-(3-chloro-2-fluorophenyl)-4-cyano-5-(2,2-dimethylpropyl)pyrrolidine-2-carbonyl]amino}-3-methoxybenzoic acid</strong> carbamoylmethyl ester as an off-white solid (62 mg, 58% yield). LCMS (ES+) m/z calcd. for C33H33Cl2F2N4O5[(M+H)+]: 673. found: 673 |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
86% | With caesium carbonate; In N,N-dimethyl-formamide; at 50℃; for 1.5h;Sealed tube; | In a 15 mL pressure tube, <strong>[1229705-06-9]4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoic acid</strong> (prepared as described in US20100152190A1, 100 mg, 162 mumol) and cesium carbonate (63.4 mg, 195 mumol) were combined with dry dimethyl formamide (5 mL). 2-Chloro-1-morpholinoethanone (26.5 mg, 21.1 muL, 162 mumol) was added to the mixture with stirring. The tube was capped and heated at 50 C. After 1.5 h, the reaction mixture was cooled down to room temperature. It was poured into water and extracted with ethyl acetate (2*). The ethyl acetate layers were combined, dried over anhydrous sodium sulfate, filtered and concentrated. The residue was purified with flash chromatography (24 g pre-packed silica gel column, eluted with ethyl acetate in hexanes) to give 4-[(2R,3S,4R,5S)-4-(4-chloro-2-fluoro-phenyl)-3-(3-chloro-2-fluoro-phenyl)-4-cyano-5-(2,2-dimethyl-propyl)-pyrrolidine-2-carbonyl]-amino}-3-methoxy-benzoic acid 2-morpholin-4-yl-2-oxo-ethyl ester as an off-white solid (104 mg, 86% yield) as product. LCMS (ES+) m/z calcd. for C37H39Cl2F2N4O6[(M+H)+]: 743. found: 743 |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
24% | In N,N-dimethyl-formamide; at 50℃;Sealed tube; | In a 15 mL pressure tube, <strong>[1229705-06-9]4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoic acid</strong> (prepared as described in US20100152190A1, 300 mg, 487 mumol) and 2-chloro-N-((2,2-dimethyl-1,3-dioxolan-4-yl)methyl)acetamide (101 mg, 487 mumol) were combined with dry dimethyl formamide (5 mL) to give a white suspension. The tube was capped and heated at 50 C. overnight. The reaction mixture was cooled down to room temperature. It was poured into water and extracted with ethyl acetate (2*). The ethyl acetate layers were combined, dried over anhydrous sodium sulfate, filtered and concentrated. The residue was purified with flash chromatography (24 g pre-packed silica gel column, eluted with methanol in ethyl acetate) to give 4-[(2R,3S,4R,5S)-4-(4-chloro-2-fluoro-phenyl)-3-(3-chloro-2-fluoro-phenyl)-4-cyano-5-(2,2-dimethyl-propyl)-pyrrolidine-2-carbonyl]-amino}-3-methoxy-benzoic acid [(2,2-dimethyl-[1,3]dioxolan-4-ylmethyl)-carbamoyl]-methyl ester as an off-white solid (92 mg, 24% yield). LCMS (ES+) m/z calcd. for C39H42Cl2F2N4O7[(M+H)+]: 787. found: 787 |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
1280 mg | With caesium carbonate; In N,N-dimethyl-formamide; at 50℃; for 0.166667h;Sealed tube; | In a 50 mL pressure tube, <strong>[1229705-06-9]4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoic acid</strong> (prepared as described in US20100152190A1, 1 g, 1.62 mmol), tert-butyl 2-bromoacetate (316 mg, 240 muL, 1.62 mmol) and cesium carbonate (634 mg, 1.95 mmol) were combined with dry dimethyl formamide (10 mL) to give a white suspension. The tube was capped and heated at 50 C. After 10 min, the reaction mixture was cooled down to room temperature, poured into water and extracted with ethyl acetate (2*). The combined ethyl acetate layers were washed with brine solution, dried over anhydrous sodium sulfate, filtered and concentrated to give 2-tert-butoxy-2-oxoethyl 4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoate as light yellow solid (1280 mg), which was used without further purification |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
66% | In a 20 mL round-bottomed flask, <strong>[1229705-06-9]4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoic acid</strong> (prepared as described in US20100152190A1, 100 mg, 162 mumol), HBTU (61.5 mg, 162 mumol) and Hunig's base (105 mg, 142 muL, 811 mumol) were combined with dry tetrahydrofuran (2 mL) to give an off-white suspension at room temperature. It was stirred at room temperature for 30 min. 1,11-Diamino-3,6,9-trioxaundecane (15.6 mg, 15.2 muL, 81.1 mumol) was added. After stirring at room temperature overnight, the reaction mixture was absorbed on small amount of silica gel, loaded on a 24 g pre-packed silica gel column and eluted with ethyl acetate to give (2R,3S,4R,5S)-4-(4-chloro-2-fluoro-phenyl)-3-(3-chloro-2-fluoro-phenyl)-4-cyano-5-(2,2-dimethyl-propyl)-pyrrolidine-2-carboxylic acid [4-(2-{2-[2-(2-amino-ethoxy)-ethoxy]-ethoxy}-ethylcarbamoyl)-2-methoxy-phenyl]-amide, dimer as white solid (148 mg, 66% yield). LCMS (ES+) m/z calcd. for C70H74Cl4F4N8O9[(M+H)+]: 1387. found: 1387. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
66% | In a 20 mL round-bottomed flask, <strong>[1229705-06-9]4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoic acid</strong> (prepared as described in US20100152190A1, 200 mg, 324 mumol) and HATU (136 mg, 357 mumol) combined with dry tetrahydrofuran (2 mL) to give an off-white suspension at room temperature. It was stirred for 30 min at room temperature. The reaction mixture was slowly added dropwise to a solution of 1,11-diamino-3,6,9-trioxaundecane (312 mg, 304 muL, 1.62 mmol) in dry tetrahydrofuran (1.5 mL) at 0 C. The reaction was done after stirring at room temperature for 20 min. It was filtered and injected into Gilson reverse-phase high-performance liquid chromatography for purification. The desired fractions was converted to free base and lyophilized to afford (2R,3S,4R,5S)-4-(4-chloro-2-fluoro-phenyl)-3-(3-chloro-2-fluoro-phenyl)-4-cyano-5-(2,2-dimethyl-propyl)-pyrrolidine-2-carboxylic acid [4-(2-{2-[2-(2-amino-ethoxy)-ethoxy]-ethoxy}-ethylcarbamoyl)-2-methoxy-phenyl]-amide as a white solid (169 mg, 66% yield). LCMS (ES+) m/z calcd. for C39H47Cl2F2N5O6[(M+H)+]: 790. found: 790 |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
General procedure: 4-((2R,3S,4R,5S)-3-(3-Chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoic acid (prepared as described in US20100152190A1, 119.9 mg, 194 mumol) was suspended in methylene chloride (5 mL), then N,N-diisopropylethylamine (156 mg, 210 muL, 1.21 mmol) was added, followed by (2-(7-aza-1H-benzotriazole-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate) (HATU, 120 mg, 316 mumol). After stirring at room temperature under argon for a few minutes, DL-alaminol (24.1 mg, 25 muL, 321 mumol) was added. The reaction progress was monitored by LC-MS. After its completion (?2 h), the reaction mixture was taken in ethyl acetate (75 mL) and water (15 mL). The layers were separated, and the organic layers were washed with saturated solution of sodium bicarbonate (10 mL), water (15 mL), brine (15 mL), and dried over anhydrous sodium sulfate. The solids were filtered off, and the crude residue was purified by flash chromatography (8 g of silica gel, eluting with 0.5-5.0% ethanol in methylene chloride) to give rac-(2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-N-(4-(1-hydroxypropan-2-ylcarbamoyl)-2-methoxyphenyl)-5-neopentylpyrrolidine-2-carboxamide (118.9 mg, 177 mumol, 90.8% yield) as white solids. MS (ES+) m/z calcd. for C34H37Cl2F2N4O4: [(M+H)+]: 673. found: 673 |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
90.8% | With N-ethyl-N,N-diisopropylamine; N-[(dimethylamino)-3-oxo-1H-1,2,3-triazolo[4,5-b]pyridin-1-yl-methylene]-N-methylmethanaminium hexafluorophosphate; In dichloromethane; at 20℃;Inert atmosphere; | 4-((2R,3S,4R,5S)-3-(3-Chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoic acid (prepared as described in US20100152190A1, 119.9 mg, 194 mumol) was suspended in methylene chloride (5 mL), then N,N-diisopropylethylamine (156 mg, 210 muL, 1.21 mmol) was added, followed by (2-(7-aza-1H-benzotriazole-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate) (HATU, 120 mg, 316 mumol). After stirring at room temperature under argon for a few minutes, DL-alaminol (24.1 mg, 25 muL, 321 mumol) was added. The reaction progress was monitored by LC-MS. After its completion (?2 h), the reaction mixture was taken in ethyl acetate (75 mL) and water (15 mL). The layers were separated, and the organic layers were washed with saturated solution of sodium bicarbonate (10 mL), water (15 mL), brine (15 mL), and dried over anhydrous sodium sulfate. The solids were filtered off, and the crude residue was purified by flash chromatography (8 g of silica gel, eluting with 0.5-5.0% ethanol in methylene chloride) to give rac-(2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-N-(4-(1-hydroxypropan-2-ylcarbamoyl)-2-methoxyphenyl)-5-neopentylpyrrolidine-2-carboxamide (118.9 mg, 177 mumol, 90.8% yield) as white solids. MS (ES+) m/z calcd. for C34H37Cl2F2N4O4: [(M+H)+]: 673. found: 673 |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
With N-ethyl-N,N-diisopropylamine; N-[(dimethylamino)-3-oxo-1H-1,2,3-triazolo[4,5-b]pyridin-1-yl-methylene]-N-methylmethanaminium hexafluorophosphate; In dichloromethane; at 20℃;Inert atmosphere; | 4-((2R,3S,4R,5S)-3-(3-Chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoic acid (prepared as described in US20100152190A1, 119.9 mg, 194 mumol) was suspended in methylene chloride (5 mL), then N,N-diisopropylethylamine (156 mg, 210 muL, 1.21 mmol) was added, followed by (2-(7-aza-1H-benzotriazole-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate) (HATU, 120 mg, 316 mumol). After stirring at room temperature under argon for a few minutes, DL-alaminol (24.1 mg, 25 muL, 321 mumol) was added. The reaction progress was monitored by LC-MS. After its completion (?2 h), the reaction mixture was taken in ethyl acetate (75 mL) and water (15 mL). The layers were separated, and the organic layers were washed with saturated solution of sodium bicarbonate (10 mL), water (15 mL), brine (15 mL), and dried over anhydrous sodium sulfate. The solids were filtered off, and the crude residue was purified by flash chromatography (8 g of silica gel, eluting with 0.5-5.0% ethanol in methylene chloride) to give rac-(2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-N-(4-(1-hydroxypropan-2-ylcarbamoyl)-2-methoxyphenyl)-5-neopentylpyrrolidine-2-carboxamide (118.9 mg, 177 mumol, 90.8% yield) as white solids. MS (ES+) m/z calcd. for C34H37Cl2F2N4O4: [(M+H)+]: 673. found: 673 |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
4-((2R,3S,4R,5S)-3-(3-Chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoic acid (prepared as described in US20100152190A1, 119.9 mg, 194 mumol) was suspended in methylene chloride (5 mL), then N,N-diisopropylethylamine (156 mg, 210 muL, 1.21 mmol) was added, followed by (2-(7-aza-1H-benzotriazole-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate) (HATU, 120 mg, 316 mumol). After stirring at room temperature under argon for a few minutes, DL-alaminol (24.1 mg, 25 muL, 321 mumol) was added. The reaction progress was monitored by LC-MS. After its completion (?2 h), the reaction mixture was taken in ethyl acetate (75 mL) and water (15 mL). The layers were separated, and the organic layers were washed with saturated solution of sodium bicarbonate (10 mL), water (15 mL), brine (15 mL), and dried over anhydrous sodium sulfate. The solids were filtered off, and the crude residue was purified by flash chromatography (8 g of silica gel, eluting with 0.5-5.0% ethanol in methylene chloride) to give rac-(2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-N-(4-(1-hydroxypropan-2-ylcarbamoyl)-2-methoxyphenyl)-5-neopentylpyrrolidine-2-carboxamide (118.9 mg, 177 mumol, 90.8% yield) as white solids. MS (ES+) m/z calcd. for C34H37Cl2F2N4O4: [(M+H)+]: 673. found: 673 |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
4-((2R,3S,4R,5S)-3-(3-Chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoic acid (prepared as described in US20100152190A1, 119.9 mg, 194 mumol) was suspended in methylene chloride (5 mL), then N,N-diisopropylethylamine (156 mg, 210 muL, 1.21 mmol) was added, followed by (2-(7-aza-1H-benzotriazole-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate) (HATU, 120 mg, 316 mumol). After stirring at room temperature under argon for a few minutes, DL-alaminol (24.1 mg, 25 muL, 321 mumol) was added. The reaction progress was monitored by LC-MS. After its completion (?2 h), the reaction mixture was taken in ethyl acetate (75 mL) and water (15 mL). The layers were separated, and the organic layers were washed with saturated solution of sodium bicarbonate (10 mL), water (15 mL), brine (15 mL), and dried over anhydrous sodium sulfate. The solids were filtered off, and the crude residue was purified by flash chromatography (8 g of silica gel, eluting with 0.5-5.0% ethanol in methylene chloride) to give rac-(2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-N-(4-(1-hydroxypropan-2-ylcarbamoyl)-2-methoxyphenyl)-5-neopentylpyrrolidine-2-carboxamide (118.9 mg, 177 mumol, 90.8% yield) as white solids. MS (ES+) m/z calcd. for C34H37Cl2F2N4O4: [(M+H)+]: 673. found: 673 |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
With N-ethyl-N,N-diisopropylamine; N-[(dimethylamino)-3-oxo-1H-1,2,3-triazolo[4,5-b]pyridin-1-yl-methylene]-N-methylmethanaminium hexafluorophosphate; hydrazine; In dichloromethane; at 20℃;Inert atmosphere; | 4-((2R,3S,4R,5S)-3-(3-Chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoic acid (prepared as described in US20100152190A1, 119.9 mg, 194 mumol) was suspended in methylene chloride (5 mL), then N,N-diisopropylethylamine (156 mg, 210 muL, 1.21 mmol) was added, followed by (2-(7-aza-1H-benzotriazole-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate) (HATU, 120 mg, 316 mumol). After stirring at room temperature under argon for a few minutes, DL-alaminol (24.1 mg, 25 muL, 321 mumol) was added. The reaction progress was monitored by LC-MS. After its completion (?2 h), the reaction mixture was taken in ethyl acetate (75 mL) and water (15 mL). The layers were separated, and the organic layers were washed with saturated solution of sodium bicarbonate (10 mL), water (15 mL), brine (15 mL), and dried over anhydrous sodium sulfate. The solids were filtered off, and the crude residue was purified by flash chromatography (8 g of silica gel, eluting with 0.5-5.0% ethanol in methylene chloride) to give rac-(2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-N-(4-(1-hydroxypropan-2-ylcarbamoyl)-2-methoxyphenyl)-5-neopentylpyrrolidine-2-carboxamide (118.9 mg, 177 mumol, 90.8% yield) as white solids. MS (ES+) m/z calcd. for C34H37Cl2F2N4O4: [(M+H)+]: 673. found: 673 |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
4-((2R,3S,4R,5S)-3-(3-Chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoic acid (prepared as described in US20100152190A1, 119.9 mg, 194 mumol) was suspended in methylene chloride (5 mL), then N,N-diisopropylethylamine (156 mg, 210 muL, 1.21 mmol) was added, followed by (2-(7-aza-1H-benzotriazole-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate) (HATU, 120 mg, 316 mumol). After stirring at room temperature under argon for a few minutes, DL-alaminol (24.1 mg, 25 muL, 321 mumol) was added. The reaction progress was monitored by LC-MS. After its completion (2 h), the reaction mixture was taken in ethyl acetate (75 mL) and water (15 mL). The layers were separated, and the organic layers were washed with saturated solution of sodium bicarbonate (10 mL), water (15 mL), brine (15 mL), and dried over anhydrous sodium sulfate. The solids were filtered off, and the crude residue was purified by flash chromatography (8 g of silica gel, eluting with 0.5-5.0% ethanol in methylene chloride) to give rac-(2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-N-(4-(1-hydroxypropan-2-ylcarbamoyl)-2-methoxyphenyl)-5-neopentylpyrrolidine-2-carboxamide (118.9 mg, 177 mumol, 90.8% yield) as white solids. MS (ES+) m/z calcd. for C34H37Cl2F2N4O4: [(M+H)+]: 673. found: 673 |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
4-((2R,3S,4R,5S)-3-(3-Chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoic acid (prepared as described in US20100152190A1, 119.9 mg, 194 mumol) was suspended in methylene chloride (5 mL), then N,N-diisopropylethylamine (156 mg, 210 muL, 1.21 mmol) was added, followed by (2-(7-aza-1H-benzotriazole-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate) (HATU, 120 mg, 316 mumol). After stirring at room temperature under argon for a few minutes, DL-alaminol (24.1 mg, 25 muL, 321 mumol) was added. The reaction progress was monitored by LC-MS. After its completion (2 h), the reaction mixture was taken in ethyl acetate (75 mL) and water (15 mL). The layers were separated, and the organic layers were washed with saturated solution of sodium bicarbonate (10 mL), water (15 mL), brine (15 mL), and dried over anhydrous sodium sulfate. The solids were filtered off, and the crude residue was purified by flash chromatography (8 g of silica gel, eluting with 0.5-5.0% ethanol in methylene chloride) to give rac-(2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-N-(4-(1-hydroxypropan-2-ylcarbamoyl)-2-methoxyphenyl)-5-neopentylpyrrolidine-2-carboxamide (118.9 mg, 177 mumol, 90.8% yield) as white solids. MS (ES+) m/z calcd. for C34H37Cl2F2N4O4: [(M+H)+]: 673. found: 673 |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
53% | With bis-(2-oxo-3-oxazolidinyl)phosphoryl chloride; triethylamine; In dichloromethane; at 20℃; | To a suspension of chiral <strong>[1229705-06-9]4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoic acid</strong> (US20100152190A1, 197 mg, 0.320 mmol,) in methylene chloride (8 mL) was added triethylamine (97.0 mg, 0.14 mL, 0.959 mmol) followed by bis(2-oxo-3-oxazolidinyl)phosphinic chloride (Aldrich, 114 mg, 0.447 mmol). The mixture was stirred at room temperature for 10 min before ethane-1,2-diol (Aldrich, 122 mg, 0.11 mL, 1.97 mmol) was added. This reaction mixture was stirred at room temperature overnight. It was then diluted with methylene chloride and washed with water, brine and concentrated to dryness. The crude material was purified by flash chromatography (eluting with hexane/ethyl acetate, 80/20 to 10/90) to give chiral 2-hydroxyethyl 4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoate as a white solid (112.2 mg, 53% yield). MS (ES+) m/z calcd. for C33H34Cl2F2N3O5[(M+H)+]: 660. found: 660 |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
With water; sodium hydroxide; In tetrahydrofuran; methanol; at 20℃; for 18h; | The reaction of (IV) with (V) (R1 = Et, 3) proceeded slower in the absence of base, and HPLC analysis showed higher level of unidentified intermediates. The formation of these intermediates was partially suppressed with catalytic amount of base. Three bases, triethylamine, DIPEA, and DABCO, were tested and worked equally well. One equivalent of the base was sufficient for the reaction to complete in 24 h, and no further improvement was observed when excess amount of base was used. Since both Cu(I) and Cu(II) salts are able to catalyze the [3+2] cycloaddition in the absence of a ligand, it is important to pre-form the metal/ligand complex to minimize the background reactions. Normally, Cu(OAc)2 and (R)-BINAP were mixed in MeTHF and stirred for 2 to 3 h before the addition of the substrates. Under these conditions, in the crude mixture, the ratio of exo : endo was -10 : 1. Short catalyst aging (eg. <30 min) led to incomplete reaction and poor exo : endo selectivity (-3 : 5). On the contrary, longer catalyst aging (eg. 20 h) resulted in faster reaction (7 h vs overnight) and an improved exo : endo ratio of -20 : 1. However, the total percentage of the minor isomers remained at 10-12 area . The improved exo : endo ratio did not lead to a better isolated yield for compound (I) at the end. The ligand screening was conducted with Cu(OAc)2 (1.0 mol%), phosphine ligand (1.1 mol%) and Nu,Nu-diisopropylethylamine (DIPEA, 1 equiv) in MeTHF at room temperature for 2 days to ensure complete conversion achieved. All reactions gave compound (XI) or (XII) (R1 = Et) as the major products (Table 1), though level of other minor isomers varied slightly. The reaction mixtures were then treated with aqueous sodium hydroxide (NaOH) to convert both compound (XI) and (XII) (R1 = Et; 6 and 7) to compound (I). The resulting mixture was analyzed by chiral HPLC. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
With water; sodium hydroxide; In tetrahydrofuran; methanol; at 20℃; for 18h; | The reaction of (IV) with (V) (R1 = Et, 3) proceeded slower in the absence of base, and HPLC analysis showed higher level of unidentified intermediates. The formation of these intermediates was partially suppressed with catalytic amount of base. Three bases, triethylamine, DIPEA, and DABCO, were tested and worked equally well. One equivalent of the base was sufficient for the reaction to complete in 24 h, and no further improvement was observed when excess amount of base was used. Since both Cu(I) and Cu(II) salts are able to catalyze the [3+2] cycloaddition in the absence of a ligand, it is important to pre-form the metal/ligand complex to minimize the background reactions. Normally, Cu(OAc)2 and (R)-BINAP were mixed in MeTHF and stirred for 2 to 3 h before the addition of the substrates. Under these conditions, in the crude mixture, the ratio of exo : endo was -10 : 1. Short catalyst aging (eg. <30 min) led to incomplete reaction and poor exo : endo selectivity (-3 : 5). On the contrary, longer catalyst aging (eg. 20 h) resulted in faster reaction (7 h vs overnight) and an improved exo : endo ratio of -20 : 1. However, the total percentage of the minor isomers remained at 10-12 area . The improved exo : endo ratio did not lead to a better isolated yield for compound (I) at the end. The ligand screening was conducted with Cu(OAc)2 (1.0 mol%), phosphine ligand (1.1 mol%) and Nu,Nu-diisopropylethylamine (DIPEA, 1 equiv) in MeTHF at room temperature for 2 days to ensure complete conversion achieved. All reactions gave compound (XI) or (XII) (R1 = Et) as the major products (Table 1), though level of other minor isomers varied slightly. The reaction mixtures were then treated with aqueous sodium hydroxide (NaOH) to convert both compound (XI) and (XII) (R1 = Et; 6 and 7) to compound (I). The resulting mixture was analyzed by chiral HPLC. | |
With water; sodium hydroxide; In tetrahydrofuran; methanol; at 20℃; for 18h; | A 500-mL, round bottomed flask equipped with a magnetic stirrer and nitrogen inlet/bubbler was charged with copper(II) acetate (150 mg, 0.826 mmol), (R)-BINAP (560 mg, 0.899 mmol), and 2-methyltetrahydrofuran (120 mL). The suspension was stirred at room temperature under N2 for 3 h when a clear blue solution was obtained. Then 12.0 mL (68.7 mmol) of N,N- diisopropylethylamine was added, followed by 20.0 g (64.5 mmol) of Compound (1) and 24.0 g (71.8 mmol) of Compound (2). The suspension was stirred at room temperature under N2 for 18 h, and LCMS analysis indicated complete reaction. The reaction mixture was diluted with 100 mL of 5% ammonium acetate solution and stirred for 15 min, then poured into a 500-mL separatory funnel. The organic phase separated was washed with an additional 5% ammonium acetate solution (100 mL), then with 100 mL of 5% sodium chloride solution (100 mL), and ? - 27 - concentrated at 40 C under reduced pressure to a thick syrup (ca. 60 g ). This syrup (containing 6 and 7) was dissolved in tetrahydrofuran (120 mL), methanol (60.0 mL), and water (6.00 mL). Then sodium hydroxide (50% solution, 6.00 mL, 114 mmol) was added dropwise. The mixture was stirred at room temperature for 18 h. LCMS and chiral HPLC indicated complete hydrolysis and isomerization. The reaction mixture was acidified with 20.0 mL (349 mmol) of acetic acid, and then concentrated at 40 C under reduced pressure to remove ca. 80 mL of solvent. The residue was diluted with 2-propanol (200 mL), and further concentrated to remove ca. 60 mL of solvent, and then water (120 mL) was added. The slurry was stirred under reflux for 1 h, at room temperature overnight, then filtered and the flask was rinsed with of 2-propanol- water (2: 1) (20.0 mL). The filter cake was washed with 2-propanol- water (1: 1) (2 x 100 mL = 200 mL), and with water (2 x 200 mL = 400 mL), then vacuum oven dried at 60 C to give 33.48 g (84.2% yield) of crude Compound 5 as a white solid ; 99.26% pure and 87.93% ee as judged by LCMS and chiral HPLC analysis. Compound 6 (exo cycloaddition product, 2,5-cis): 1H NMR (400 MHz, CDC13) delta 9.66 (brs, 1H), 8.42 (d, J = 8.3 Hz, 1H), 7.89 (m, 1H), 7.65 (dd, J = 8.6, 1.8 Hz, 1H), 7.55 (d, J = 1.8 Hz, 1H), 7.40 (m, 1H), 7.32 (td, J = 8.3, 1.5 Hz, 1H), 7.22-7.15 (m, 3H), 4.45 (m, 2H), 4.36 (q, J = 7.2 Hz, 2H), 4.25 (m, 1H), 3.91 (s, 3H), 1.39 (t, J = 7.2 Hz, 3H), 1.30 (dd, J = 14.2, 9.3 Hz, 1H), 0.92 (s, 9H), 0.84 (d, J = 14.2 Hz, 1H). Compound 7 (endo cycloaddition product, 2,5-cis): 1H NMR (400 MHz, CDC13) delta 9.97 (brs, 1H), 8.30 (d, J = 8.4 Hz, 1H), 7.65 (dd, J = 8.3, 1.8 Hz, 1H), 7.56 (d, J = 1.7 Hz, 1H), 7.51 (m, 1H), 7.43 (t, J = 8.4 Hz, 1H), 7.23 (m, 1H), 7.17 (dd, J =12.6, 2.0 Hz, 1H), 7.11 (m, 1H), 6.89 (td, J = 8.1, 1.2 Hz, 1H), 5.05 (dd, J = 10.8, 2.1 Hz, 1H), 4.53 (d, J = 10.8 Hz, 1H), 4.37 (q, J = 7.2 Hz, 2H), 4.22 (d, J = 8.7 Hz, 1H), 3.95 (s, 3H), 1.85 (dd, J = 14.1, 8.7 Hz, 1H), 1.48 (d, J =14.1 Hz, 1H), 1.40 (t, J = 7.2 Hz, 1H), 0.97 (s, 9H). |
Tags: 1229705-06-9 synthesis path| 1229705-06-9 SDS| 1229705-06-9 COA| 1229705-06-9 purity| 1229705-06-9 application| 1229705-06-9 NMR| 1229705-06-9 COA| 1229705-06-9 structure
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